Question 1

Which of the following is not an environmental factor that would affect the growth of E. coli?

Accepted Answer

A)  temperature 
B)  antibiotics produced from other bacterial species 
C)  concentration of other bacteria 
D)  availability of nutrients 
E)  generation time of E. coli 
A)  temperature 
B)  antibiotics produced from other bacterial species 
C)  concentration of other bacteria 
D)  availability of nutrients 
E)  generation time of E. coli

Question 2

How do halophiles combat osmotic stress?

Accepted Answer

A)  They enrich their plasma membrane with basic compounds to attract OH- ions to their cell surface. 
B)  They use proton pumps to export excess protons from the cytoplasm. 
C)  They keep a high concentration of organic materials and ions in their cytoplasm. 
D)  They use plasma membrane lipids that are highly branched and saturated. 
E)  They build special monolayer plasma membranes. 
A)  They enrich their plasma membrane with basic compounds to attract OH- ions to their cell surface. 
B)  They use proton pumps to export excess protons from the cytoplasm. 
C)  They keep a high concentration of organic materials and ions in their cytoplasm. 
D)  They use plasma membrane lipids that are highly branched and saturated. 
E)  They build special monolayer plasma membranes.

Question 3

The minimum temperature needed to kill all microbes in a sample within ten minutes is called the

Accepted Answer

A)  thermal death point. 
B)  thermal death time. 
C)  essential reduction point. 
D)  critical death time. 
E)  decimal reduction time. 
A)  thermal death point. 
B)  thermal death time. 
C)  essential reduction point. 
D)  critical death time. 
E)  decimal reduction time.

Question 4

Which of the following could result in the wrong patient diagnosis and improper treatment?

Accepted Answer

A)  carefully sampling only the site in question without touching other body structures or surface sites 
B)  using a cotton tip swap to collect a suspected pathogen 
C)  wearing gloves 
D)  proper hand washing before and after specimen collection 
E)  quickly sealing samples in containers 
A)  carefully sampling only the site in question without touching other body structures or surface sites 
B)  using a cotton tip swap to collect a suspected pathogen 
C)  wearing gloves 
D)  proper hand washing before and after specimen collection 
E)  quickly sealing samples in containers

Question 5

Which of the following types of organisms is associated with foodborne illness?

Accepted Answer

A)  psychrotrophs 
B)  extreme thermophiles 
C)  mesophiles 
D)  psychrophiles 
E)  thermophiles 
A)  psychrotrophs 
B)  extreme thermophiles 
C)  mesophiles 
D)  psychrophiles 
E)  thermophiles

Question 6

Which type of organism gets energy from breaking down nutrients and uses CO2 as a carbon source?

Accepted Answer

A)  lithotroph 
B)  chemoautotroph 
C)  photoheterotroph 
D)  photoautotroph 
E)  chemoheterotroph 
A)  lithotroph 
B)  chemoautotroph 
C)  photoheterotroph 
D)  photoautotroph 
E)  chemoheterotroph

Question 7

What is the purpose of the streak plate technique?

Accepted Answer

A)  to obtain a pure culture from a mixed or unknown source 
B)  to determine the concentration of a microbial sample 
C)  to identify the correct concentration of nutrients needed for optimal bacterial growth 
D)  to identify the best temperature for growing a particular microbe 
E)  to ascertain the age of a particular culture 
A)  to obtain a pure culture from a mixed or unknown source 
B)  to determine the concentration of a microbial sample 
C)  to identify the correct concentration of nutrients needed for optimal bacterial growth 
D)  to identify the best temperature for growing a particular microbe 
E)  to ascertain the age of a particular culture

Question 8

Nutrients that a cell needs to build new cells and make up a bulk of a cell's dry weight are called

Accepted Answer

A)  small nutrients. 
B)  trace nutrients. 
C)  micronutrients. 
D)  tiny nutrients. 
E)  essential nutrients. 
A)  small nutrients. 
B)  trace nutrients. 
C)  micronutrients. 
D)  tiny nutrients. 
E)  essential nutrients.

Question 9

Which temperature group are most pathogens associated with?

Accepted Answer

A)  psychrotrophs 
B)  thermophiles 
C)  extreme thermophiles 
D)  psychrophiles 
E)  mesophiles 
A)  psychrotrophs 
B)  thermophiles 
C)  extreme thermophiles 
D)  psychrophiles 
E)  mesophiles

Question 10

In which growth phase do bacteria produce antibiotics and endospores?

Accepted Answer

A)  death phase 
B)  log phase 
C)  lag phase 
D)  acculturation phase 
E)  stationary phase 
A)  death phase 
B)  log phase 
C)  lag phase 
D)  acculturation phase 
E)  stationary phase

Question 11

Which term represents the temperature that bacteria grow the fastest at?

Accepted Answer

A)  human body temperature 
B)  optimal 
C)  minimum 
D)  hospitable 
E)  maximum 
A)  human body temperature 
B)  optimal 
C)  minimum 
D)  hospitable 
E)  maximum

Question 12

Which of the following microbes is not correctly matched to an acceptable control method?

Accepted Answer

A)  Mycobacteria: hydrogen peroxide 
B)  Prions: chlorine treatment 
C)  Enveloped virus: glutaraldehyde- based detergents 
D)  Endospore: autoclaving 
E)  Protozoan: boiling water 
A)  Mycobacteria: hydrogen peroxide 
B)  Prions: chlorine treatment 
C)  Enveloped virus: glutaraldehyde- based detergents 
D)  Endospore: autoclaving 
E)  Protozoan: boiling water

Question 13

The pour plate method is capable of detecting an exact cell number in the plated sample.

Accepted Answer

A) True 
 B)False

Question 14

Mycobacterium tuberculosis are grown for 80 hours. During that time, they go through four generations of growth. What is the generation time?

Accepted Answer

A)  60 minutes 
B)  40 hours 
C)  10 hours 
D)  20 hours 
E)  20 minutes 
A)  60 minutes 
B)  40 hours 
C)  10 hours 
D)  20 hours 
E)  20 minutes

Question 15

Phenols are commonly used in hospitals to sterilize critical equipment.

Accepted Answer

A) True 
 B)False

Question 16

When would a microbiologist use thioglycolate?

Accepted Answer

A)  when making complex media 
B)  when making defined media 
C)  when storing differential plates 
D)  when storing plates anaerobically 
E)  when storing selective plates 
A)  when making complex media 
B)  when making defined media 
C)  when storing differential plates 
D)  when storing plates anaerobically 
E)  when storing selective plates

Question 17

When would a microbiologist want to use broth media?

Accepted Answer

A)  when growing large batches of microbes 
B)  when isolating colonies 
C)  when observing specific culture characteristics 
D)  when testing for motility 
E)  when performing the streak plate isolation technique 
A)  when growing large batches of microbes 
B)  when isolating colonies 
C)  when observing specific culture characteristics 
D)  when testing for motility 
E)  when performing the streak plate isolation technique

Question 18

Which type of organism would a microbiologist grow on defined media?

Accepted Answer

A)  fastidious organisms 
B)  organisms with complex growth requirements 
C)  pathogens from clinical samples 
D)  certain autotrophs 
E)  a broad collection of heterotrophs 
A)  fastidious organisms 
B)  organisms with complex growth requirements 
C)  pathogens from clinical samples 
D)  certain autotrophs 
E)  a broad collection of heterotrophs

Question 19

Bacterial spore formation is a type of sexual reproduction.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following pH classifications make up most of the pathogens we know today?

Accepted Answer

A)  psychrophiles 
B)  barophiles 
C)  acidophiles 
D)  neutralophiles 
E)  alkaliphiles 
A)  psychrophiles 
B)  barophiles 
C)  acidophiles 
D)  neutralophiles 
E)  alkaliphiles

Exam 7: Fundamentals of Microbial Growth

Which of the following is not an environmental factor that would affect the growth of E. coli?

How do halophiles combat osmotic stress?

The minimum temperature needed to kill all microbes in a sample within ten minutes is called the

Which of the following could result in the wrong patient diagnosis and improper treatment?

Which of the following types of organisms is associated with foodborne illness?

Which type of organism gets energy from breaking down nutrients and uses CO2 as a carbon source?

What is the purpose of the streak plate technique?

Nutrients that a cell needs to build new cells and make up a bulk of a cell's dry weight are called

Which temperature group are most pathogens associated with?

In which growth phase do bacteria produce antibiotics and endospores?

Which term represents the temperature that bacteria grow the fastest at?

Which of the following microbes is not correctly matched to an acceptable control method?

The pour plate method is capable of detecting an exact cell number in the plated sample.

Mycobacterium tuberculosis are grown for 80 hours. During that time, they go through four generations of growth. What is the generation time?

Phenols are commonly used in hospitals to sterilize critical equipment.

When would a microbiologist use thioglycolate?

When would a microbiologist want to use broth media?

Which type of organism would a microbiologist grow on defined media?

Bacterial spore formation is a type of sexual reproduction.

Which of the following pH classifications make up most of the pathogens we know today?

Introduction to Microbiology

Biochemistry Basics

Introduction to Prokaryotic Cells

Introduction to Eukaryotic Cells

Genetics

Viruses and Prions

Microbial Metabolism

Principles of Infectious Disease and Epidemiology

Host-Microbe Interactions and Pathogenesis

Innate Immunity

Adaptive Immunity

Immune System Disorders

Vaccines and Biotechnology-Based Diagnostics and Therapeutics

Antimicrobial Drugs

Respiratory System Infections

Skin and Eye Infections

Nervous System Infections

Digestive System Infections

Urinary and Reproductive System Infections

Cardiovascular and Lymphatic Infections

Filters

Which type of organism gets energy from breaking down nutrients and uses CO₂ as a carbon source?