Question 1

Gene therapy has been successful in curing some individuals of autoimmune disorder and sickle cell disease.

Accepted Answer

A) True 
 B)False  True

Question 2

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You would use this tool if you wanted to check if a particular gene was contained in an organism's genome.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR A

Question 3

Which of the following is characteristic of cDNA?

Accepted Answer

A)  a double stranded DNA copy of a single-stranded mRNA molecule 
B)  a single stranded DNA copy of a single-stranded mRNA molecule 
C)  a single stranded mRNA copy of a double-stranded mRNA molecule 
D)  a single stranded mRNA copy of a single-stranded mRNA molecule 
A)  a double stranded DNA copy of a single-stranded mRNA molecule 
B)  a single stranded DNA copy of a single-stranded mRNA molecule 
C)  a single stranded mRNA copy of a double-stranded mRNA molecule 
D)  a single stranded mRNA copy of a single-stranded mRNA molecule A

Question 4

How many base pairs is the general length of the DNA sequence that is recognized by restriction endonucleases?

Accepted Answer

A)  1 to 2 
B)  4 to 8 
C)  10 to 15 
D)  24 to 32 
A)  1 to 2 
B)  4 to 8 
C)  10 to 15 
D)  24 to 32

Question 5

Figure 14-1  
-According to Figure 14-1, which of the following was used to digest a DNA fragment that results in the following sequence? AATTC---------G
G---------CTTAA

Accepted Answer

A)  EcoRI 
B)  sticky end 
C)  nick in the sugar-phosphate backbone 
D)  recombinant plasmid 
A)  EcoRI 
B)  sticky end 
C)  nick in the sugar-phosphate backbone 
D)  recombinant plasmid

Question 6

Match each term with its definition.
-restriction fragments

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 7

When using embryonic germ-line cells to create transgenic mice, the offspring of the genetically modified animal will have all transgenic cells in their bodies.

Accepted Answer

A) True 
 B)False

Question 8

Match each term with the most appropriate macromolecule being studied or manipulate
-fingerprinting

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein.

Question 9

Match the names of the processes with the descriptions.
-This methodology lets you study the complete set of protein that can be expressed by an organism's genome.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 10

Some restriction endonucleases cut the DNA in such a way that short, single-stranded regions are created. What are these regions called?

Accepted Answer

A)  blunt ends 
B)  tacky ends 
C)  hydrogen-bonding ends 
D)  sticky ends 
A)  blunt ends 
B)  tacky ends 
C)  hydrogen-bonding ends 
D)  sticky ends

Question 11

Which of the following describes DNA ligase?

Accepted Answer

A)  the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce an original DNA molecule 
B)  the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
C)  the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
D)  the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant RNA molecule 
A)  the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce an original DNA molecule 
B)  the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
C)  the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
D)  the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant RNA molecule

Question 12

What drawback have researchers noted in the cloning of domestic animals?

Accepted Answer

A)  The cloned animals often suffer from birth defects. 
B)  The cloned animals do not resemble the parent. 
C)  The cloned animals do not reach adulthood. 
D)  The cloned animals are not viable. 
A)  The cloned animals often suffer from birth defects. 
B)  The cloned animals do not resemble the parent. 
C)  The cloned animals do not reach adulthood. 
D)  The cloned animals are not viable.

Question 13

Match the names of the processes with the descriptions.
-You use this technique to produce recombinant DNA.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 14

Match each term with the most appropriate macromolecule being studied or manipulate
-agarose gel electrophoresis

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D)  protein.

Question 15

Some genetically modified tobacco plants can express the light-producing chemicals present in fireflies.

Accepted Answer

A) True 
 B)False

Question 16

Define the purpose of cloning a gene into a plasmid cloning vector.

Accepted Answer

By cloning a gene into a vector, you can

Question 17

Why is it important to have an antibiotic resistance gene in the cloning plasmid?

Accepted Answer

A)  It provides a way for researchers to sort the bacteria that have the cloning plasmid from the bacteria that do not have the cloning plasmid. 
B)  It provides a way to sort bacteria that have the gene of interest from the bacteria that have just an "empty" cloning plasmid. 
C)  It makes the bacteria resistant to the antibiotic. 
D)  It aids scientists in developing new antibiotic treatments. 
A)  It provides a way for researchers to sort the bacteria that have the cloning plasmid from the bacteria that do not have the cloning plasmid. 
B)  It provides a way to sort bacteria that have the gene of interest from the bacteria that have just an "empty" cloning plasmid. 
C)  It makes the bacteria resistant to the antibiotic. 
D)  It aids scientists in developing new antibiotic treatments.

Question 18

Match each term with the most appropriate macromolecule being studied or manipulate
-genomics

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
D) protein. 
A) DNA 
B) mRNA/cDNA 
C) protein 
D) protein.

Question 19

Which of the following refer to nonrecombinant plasmids?

Accepted Answer

A)  resealed cloning vectors with no DNA fragment inserted 
B)  plasmids with DNA fragments inserted into the cloning vector 
C)  plasmids with no DNA fragment inserted 
D)  resealed cloning vectors with DNA fragments inserted into the cloning vector 
A)  resealed cloning vectors with no DNA fragment inserted 
B)  plasmids with DNA fragments inserted into the cloning vector 
C)  plasmids with no DNA fragment inserted 
D)  resealed cloning vectors with DNA fragments inserted into the cloning vector

Question 20

Suppose that multiple fragments from a genome have been cloned into bacteria. How can researchers identify the bacteria that contain the particular sequence they are interested in?

Accepted Answer

A)  by sequencing all the bacterial DNA 
B)  by DNA hybridization, using a labelled probe 
C)  by observing that the bacteria will produce white colonies on X-gal 
D)  by isolating the DNA of interest before transformation 
A)  by sequencing all the bacterial DNA 
B)  by DNA hybridization, using a labelled probe 
C)  by observing that the bacteria will produce white colonies on X-gal 
D)  by isolating the DNA of interest before transformation

Exam 14: Dna Technologies

Gene therapy has been successful in curing some individuals of autoimmune disorder and sickle cell disease.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You would use this tool if you wanted to check if a particular gene was contained in an organism's genome.

Which of the following is characteristic of cDNA?

How many base pairs is the general length of the DNA sequence that is recognized by restriction endonucleases?

Figure 14-1 -According to Figure 14-1, which of the following was used to digest a DNA fragment that results in the following sequence? AATTC---------G G---------CTTAA

Match each term with its definition. -restriction fragments

When using embryonic germ-line cells to create transgenic mice, the offspring of the genetically modified animal will have all transgenic cells in their bodies.

Match each term with the most appropriate macromolecule being studied or manipulate -fingerprinting

Match the names of the processes with the descriptions. -This methodology lets you study the complete set of protein that can be expressed by an organism's genome.

Some restriction endonucleases cut the DNA in such a way that short, single-stranded regions are created. What are these regions called?

Which of the following describes DNA ligase?

What drawback have researchers noted in the cloning of domestic animals?

Match the names of the processes with the descriptions. -You use this technique to produce recombinant DNA.

Match each term with the most appropriate macromolecule being studied or manipulate -agarose gel electrophoresis

Some genetically modified tobacco plants can express the light-producing chemicals present in fireflies.

Define the purpose of cloning a gene into a plasmid cloning vector.

Why is it important to have an antibiotic resistance gene in the cloning plasmid?

Match each term with the most appropriate macromolecule being studied or manipulate -genomics

Which of the following refer to nonrecombinant plasmids?

Suppose that multiple fragments from a genome have been cloned into bacteria. How can researchers identify the bacteria that contain the particular sequence they are interested in?

Light and Life

The Cell: an Overview

Energy and Enzymes

Cell Membranes and Signaling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

The Chromosomal Basis of Mendelian Inheritance

Genetic Linkage, Sex-Linkage, and Other Non-Mendelian Inheritance Mechanisms

DNA Structure, Replication, and Repair

Gene Structure Expression, and Mutation

Regulation of Gene Expression

Genomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Speciation and Macroevolution

Systematics and Phylogenetics: Revealing the Tree of Life

Humans and Evolution

Defining Life and Its Origins

Viruses, Viroids, and Prions: Infectious Biological Particles

Bacteria and Archaea

Protists

Fungi

Plants

Animals

Conservation of Biodiversity

Population Ecology

Species Interactions and Community Ecology

Ecosystems

Animal Behaviour

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Animal Nutrition

Gas Exchange: the Respiratory System

Internal Transport: the Circulatory System

Regulation of the Internal Environment: Water, Solutes, and Temperature

Control of Animal Processes: Endocrine Control

Animal Reproduction

Control of Animal Processes: Neural Control

Muscles, Skeletons, and Body Movements

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