Question 1

What RNAs do each eukaryotic RNA polymerase produce?

Accepted Answer

A) RNA pol I - mRNA RNA pol II - tRNA and 5s rRNA
RNA pol III - rRNA 
B) RNA pol I - rRNA RNA pol II - tRNA and 5s rRNA
RNA pol III - mRNA 
C) RNA pol I - mRNA RNA pol II - rRNA
RNA pol III - tRNA and 5s rRNA 
D) RNA pol I - tRNA and 5s rRNA RNA pol II - mRNA
RNA pol III - rRNA 
A) RNA pol I - mRNA RNA pol II - tRNA and 5s rRNA
RNA pol III - rRNA 
B) RNA pol I - rRNA RNA pol II - tRNA and 5s rRNA
RNA pol III - mRNA 
C) RNA pol I - mRNA RNA pol II - rRNA
RNA pol III - tRNA and 5s rRNA 
D) RNA pol I - tRNA and 5s rRNA RNA pol II - mRNA
RNA pol III - rRNA

Question 2

What enables the splicing of group I and II introns?

Accepted Answer

A) Spliceosomes 
B) Ribozymes 
C) snRNA 
D) Poly-A tail 
A) Spliceosomes 
B) Ribozymes 
C) snRNA 
D) Poly-A tail

Question 3

What modifications act to stabilize eukaryotic mRNA?

Accepted Answer

A) Alternative splicing 
B) RNA editing 
C) 5' capping 
D) Polyadenylation 
E) Trimming 
A) Alternative splicing 
B) RNA editing 
C) 5' capping 
D) Polyadenylation 
E) Trimming

Question 4

What is the catalytic subunit of prokaryotic RNA polymerase?

Accepted Answer

A) a 
B) b 
C) b' 
D) $\sigma$ 
A) a 
B) b 
C) b' 
D) $\sigma$

Question 5

A ribozyme is a catalytic protein that assists in the processing of tRNA.

Accepted Answer

A) True 
 B)False

Question 6

The holozyme is formed when the core enzyme associates with

Accepted Answer

A) Start codons 
B) b 
C) b' 
D) $\sigma$ 
E) a 
A) Start codons 
B) b 
C) b' 
D) $\sigma$ 
E) a

Question 7

What is the difference between the allosteric and torpedo models of eukaryotic transcritponal termination?

Accepted Answer

A)  The allosteric model is that RNA pol II becomes destabilized after transcription of the poly A signal sequence while the torpedo model is that the polymerase is physically removed from the DNA 
B)  The torpedo model is that RNA pol II becomes destabilized after transcription of the poly A signal sequence while the allosteric model is that the polymerase is physically removed from the DNA 
C)  The allosteric model is that RNA pol II becomes destabilized after transcription of the stop codon sequence while the torpedo model is that the polymerase is physically removed from the DNA 
D)  The torpedo model is that RNA pol II becomes destabilized after transcription of the stop codon while the allosteric model is that the polymerase is physically removed from the DNA 
E)  The only difference between the two models is the proteins that are involved in removing the RNA pol II from the DNA 
A)  The allosteric model is that RNA pol II becomes destabilized after transcription of the poly A signal sequence while the torpedo model is that the polymerase is physically removed from the DNA 
B)  The torpedo model is that RNA pol II becomes destabilized after transcription of the poly A signal sequence while the allosteric model is that the polymerase is physically removed from the DNA 
C)  The allosteric model is that RNA pol II becomes destabilized after transcription of the stop codon sequence while the torpedo model is that the polymerase is physically removed from the DNA 
D)  The torpedo model is that RNA pol II becomes destabilized after transcription of the stop codon while the allosteric model is that the polymerase is physically removed from the DNA 
E)  The only difference between the two models is the proteins that are involved in removing the RNA pol II from the DNA

Question 8

The formation of open complex usually forms in GC rich regions of DNA due to the decreased number of hydrogen bonds.

Accepted Answer

A) True 
 B)False

Question 9

RNA is complimentary to

Accepted Answer

A) Regulatory sequences 
B) Termination sequences 
C) The coding strand of DNA 
D) The template strand of DNA 
A) Regulatory sequences 
B) Termination sequences 
C) The coding strand of DNA 
D) The template strand of DNA

Question 10

The RNA polymerase forms a closed complex during the process of elongation during transcription.

Accepted Answer

A) True 
 B)False

Question 11

A gene that undergoes rho-dependent termination has a mutation in the rut site. How will this influence transcriptional activity?

Accepted Answer

A) Transcription will terminate normally as Rho termiantion relies on the rut site to enhance termination; it is not essential for termination 
B) Transcription will stop immediately because RNA polymerase will sense there is a mutation 
C) Transcription will continue until another termination sequence, either Rho dependent or independent is reached 
D) There will be no transcription initiation 
A) Transcription will terminate normally as Rho termiantion relies on the rut site to enhance termination; it is not essential for termination 
B) Transcription will stop immediately because RNA polymerase will sense there is a mutation 
C) Transcription will continue until another termination sequence, either Rho dependent or independent is reached 
D) There will be no transcription initiation

Question 12

What would be the effect on in vitro transcription if you replaced the entire TFIID protein with the single subunit TBP?

Accepted Answer

A) Transcription could not be terminated 
B) The initiation complex could not form 
C) RNA polymerase II would never be recruited to carry out elongation 
D) There would be no effect 
A) Transcription could not be terminated 
B) The initiation complex could not form 
C) RNA polymerase II would never be recruited to carry out elongation 
D) There would be no effect

Question 13

Which molecules are not part of the closed promoter complex?

Accepted Answer

A) RNA polymerase 
B) Transcription factors 
C) Double helix DNA 
D) Single-stranded DNA 
A) RNA polymerase 
B) Transcription factors 
C) Double helix DNA 
D) Single-stranded DNA

Question 14

In eukaryotic organisms, the processing of the 45S rRNA into 5.8S, 18S, and 28S rRNA occurs where?

Accepted Answer

A) In the cytoplasm 
B) In the nucleolus 
C) In the endoplasmic reticulum 
D) In the Golgi body 
E) Throughout the cell 
A) In the cytoplasm 
B) In the nucleolus 
C) In the endoplasmic reticulum 
D) In the Golgi body 
E) Throughout the cell

Question 15

Which region of DNA contains the coding information for a protein in a eukaryote?

Accepted Answer

A) Exons 
B) Introns 
C) Enhancers 
D) Promoters 
A) Exons 
B) Introns 
C) Enhancers 
D) Promoters

Question 16

If a nucleotide in a eukaryotic mRNA coding sequence does not appear in the genomic DNA sequence the most likely modification to have occurred is

Accepted Answer

A) Alternative splicing 
B) RNA editing 
C) 5' capping 
D) Polyadenylation 
E) Trimming 
A) Alternative splicing 
B) RNA editing 
C) 5' capping 
D) Polyadenylation 
E) Trimming

Question 17

A mutation in TFIIIE could result in

Accepted Answer

A) a failure of the RNA polymerase to bind to TFIIB 
B) a failure of the RNA polymerase to maintain an closed complex 
C) a failure of the RNA polymerase to maintain an open complex 
D) no effect on transcription 
A) a failure of the RNA polymerase to bind to TFIIB 
B) a failure of the RNA polymerase to maintain an closed complex 
C) a failure of the RNA polymerase to maintain an open complex 
D) no effect on transcription

Question 18

What basal transcription factor is a helicase?

Accepted Answer

A) TFIID 
B) TFIIH 
C) TFIIF 
D) TFIIB 
A) TFIID 
B) TFIIH 
C) TFIIF 
D) TFIIB

Question 19

Which RNA encodes the sequence of amino acids for a functional protein?

Accepted Answer

A) tRNA 
B) snRNA 
C) mRNA 
D) rRNA 
E) scRNA 
A) tRNA 
B) snRNA 
C) mRNA 
D) rRNA 
E) scRNA

Question 20

Most eukaryotic genes are collinear.

Accepted Answer

A) True 
B) False 
C) It is unknown at this time 
A) True 
B) False 
C) It is unknown at this time

Exam 12: Gene Transcription and RNA Modification

What RNAs do each eukaryotic RNA polymerase produce?

What enables the splicing of group I and II introns?

What modifications act to stabilize eukaryotic mRNA?

What is the catalytic subunit of prokaryotic RNA polymerase?

A ribozyme is a catalytic protein that assists in the processing of tRNA.

The holozyme is formed when the core enzyme associates with

What is the difference between the allosteric and torpedo models of eukaryotic transcritponal termination?

The formation of open complex usually forms in GC rich regions of DNA due to the decreased number of hydrogen bonds.

RNA is complimentary to

The RNA polymerase forms a closed complex during the process of elongation during transcription.

A gene that undergoes rho-dependent termination has a mutation in the rut site. How will this influence transcriptional activity?

What would be the effect on in vitro transcription if you replaced the entire TFIID protein with the single subunit TBP?

Which molecules are not part of the closed promoter complex?

In eukaryotic organisms, the processing of the 45S rRNA into 5.8S, 18S, and 28S rRNA occurs where?

Which region of DNA contains the coding information for a protein in a eukaryote?

If a nucleotide in a eukaryotic mRNA coding sequence does not appear in the genomic DNA sequence the most likely modification to have occurred is

A mutation in TFIIIE could result in

What basal transcription factor is a helicase?

Which RNA encodes the sequence of amino acids for a functional protein?

Most eukaryotic genes are collinear.

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and Bacteriophages

Variation in Chromosome Structure and Number

Molecular Structure of DNA and RNA

Chromosome Organization and Molecular Structure

DNA Replication

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional Regulation

Gene Regulation in Eukaryotes II: Epigenetics and Regulation at the RNA Level

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular Level

DNA Technologies

Biotechnology

Genomics I: Analysis of DNA

Genomics II: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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