Question 1

How many DNA polymerases are found in prokaryotes?

Accepted Answer

A) 5 
B) 7 
C) 9 
D) 12 
A) 5 
B) 7 
C) 9 
D) 12

Question 2

The problem in synthesizing the 3' end of chromosomes is solved by the use of the telomerase enzyme.

Accepted Answer

A) True 
 B)False

Question 3

Replication usually begins in GC rich regions due to the presence of only 2 hydrogen bonds between the bases.

Accepted Answer

A) True 
 B)False

Question 4

Attaches adjacent Okazaki fragments, forming a continuous DNA strand.

Accepted Answer

A) DNA ligase 
B) DNA primase 
C) Topoisomerase 
D) DNA polymerase I 
E) DNA polymerase III 
A) DNA ligase 
B) DNA primase 
C) Topoisomerase 
D) DNA polymerase I 
E) DNA polymerase III

Question 5

DNA polymerases are unable to bind to what areas of the chromosome?

Accepted Answer

A)  Centromeres 
B)  3' end of telomeres 
C)  Origins of replication 
A)  Centromeres 
B)  3' end of telomeres 
C)  Origins of replication

Question 6

DNA polymerases add new nucleotides in what direction?

Accepted Answer

A) 5' to 3' 
B) 3 ' to 5' 
C) Both directions 
A) 5' to 3' 
B) 3 ' to 5' 
C) Both directions

Question 7

The Meselson-Stahl experiments supported the model of dispersive DNA replication.

Accepted Answer

A) True 
 B)False

Question 8

The Meselson-Stahl experiments used 35S radioisotopes to determine the mechanism of DNA replication.

Accepted Answer

A) True 
 B)False

Question 9

The proofreading of the DNA occurs in the _________.

Accepted Answer

A) 5' to 3' direction 
B) 3' to 5' direction 
C) Both directions 
A) 5' to 3' direction 
B) 3' to 5' direction 
C) Both directions

Question 10

GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The primer used for replication in vitro is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?

Accepted Answer

A) Left, left 
B) Right, left 
C) Left, right 
D) Right, right 
A) Left, left 
B) Right, left 
C) Left, right 
D) Right, right

Question 11

In eukaryotes, which of the following is similar to the oriC region of prokaryotes?

Accepted Answer

A) Dam 
B) ARS elements 
C) Promoters 
D) Telomeres 
A) Dam 
B) ARS elements 
C) Promoters 
D) Telomeres

Question 12

Prevents supercoiling ahead of the replication fork.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 13

The first generation of replication in the Meselson and Stahl experiment disproved which theory of replication?

Accepted Answer

A) Semiconservative 
B) Conservative 
C) Dispersive 
D) None-it took more than one generation to disprove the theory 
A) Semiconservative 
B) Conservative 
C) Dispersive 
D) None-it took more than one generation to disprove the theory

Question 14

Responsible for the majority of DNA replication.

Accepted Answer

A) DNA ligase 
B) DNA primase 
C) Topoisomerase 
D) DNA polymerase I 
E) DNA polymerase III 
A) DNA ligase 
B) DNA primase 
C) Topoisomerase 
D) DNA polymerase I 
E) DNA polymerase III

Question 15

The purpose of DNA replication is to produce ______.

Accepted Answer

A) Two daughter strands 
B) Two parental strands 
C) Two template strands 
D) None of the answers are correct 
A) Two daughter strands 
B) Two parental strands 
C) Two template strands 
D) None of the answers are correct

Question 16

A primosome consists of a polymerase and a single-stranded binding protein.

Accepted Answer

A) True 
 B)False

Question 17

The synthesis of the daughter strand of DNA occurs away from the replication fork in the leading strand.

Accepted Answer

A) True 
 B)False

Question 18

After the action of the helicase, single-stranded binding proteins keep the parental DNA strands from reforming a double-helix.

Accepted Answer

A) True 
 B)False

Question 19

You extract DNA from an E.coli cell and observe it is hemi-methylated.Which strand of DNA is older?

Accepted Answer

A) The methylated strand 
B) The strand that is not methylated 
C) Neither-they are the same "age." 
A) The methylated strand 
B) The strand that is not methylated 
C) Neither-they are the same "age."

Question 20

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Accepted Answer

A) 5' to 3' 
B) 3' to 5' 
C) They remain stationary 
A) 5' to 3' 
B) 3' to 5' 
C) They remain stationary

Exam 11: DNA Replication

How many DNA polymerases are found in prokaryotes?

The problem in synthesizing the 3' end of chromosomes is solved by the use of the telomerase enzyme.

Replication usually begins in GC rich regions due to the presence of only 2 hydrogen bonds between the bases.

Attaches adjacent Okazaki fragments, forming a continuous DNA strand.

DNA polymerases are unable to bind to what areas of the chromosome?

DNA polymerases add new nucleotides in what direction?

The Meselson-Stahl experiments supported the model of dispersive DNA replication.

The Meselson-Stahl experiments used 35S radioisotopes to determine the mechanism of DNA replication.

The proofreading of the DNA occurs in the _________.

GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The primer used for replication in vitro is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?

In eukaryotes, which of the following is similar to the oriC region of prokaryotes?

Prevents supercoiling ahead of the replication fork.

The first generation of replication in the Meselson and Stahl experiment disproved which theory of replication?

Responsible for the majority of DNA replication.

The purpose of DNA replication is to produce ______.

A primosome consists of a polymerase and a single-stranded binding protein.

The synthesis of the daughter strand of DNA occurs away from the replication fork in the leading strand.

After the action of the helicase, single-stranded binding proteins keep the parental DNA strands from reforming a double-helix.

You extract DNA from an E.coli cell and observe it is hemi-methylated.Which strand of DNA is older?

DNA helicase enzymes move in what direction along the DNA during DNA replication?

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria and Bacteriophages

Variation in Chromosome Structure and Number

Molecular Structure of DNA and RNA

Chromosome Organization and Molecular Structure

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional Regulation

Gene Regulation in Eukaryotes II: Epigenetics and Regulation at the RNA Level

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular Level

DNA Technologies

Biotechnology

Genomics I: Analysis of DNA

Genomics II: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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The Meselson-Stahl experiments used ³⁵S radioisotopes to determine the mechanism of DNA replication.