Question 1

What is the correct order for the steps in transduction?

Accepted Answer

A)  1) Phage infects cell 2) Phage transported DNA recombines with chromosome of new cell 3) Host DNA digested into fragments 4) Some phage accidently carry host DNA fragment 5) Phage infects new cell 
B)  1) Phage infects cell 2) Phage transported DNA recombines with chromosome of new cell 3) Host DNA digested into fragments 4) Phage infects new cell 5) Some phage accidently carry host DNA fragment 
C)  1) Phage infects cell 2) Host DNA digested into fragments 3) Some phage accidently carry host DNA fragment 4) Phage infects new cell 5) Phage transported DNA recombines with chromosome of new cell 
D)  1) Phage infects cell 2) Phage transported DNA recombines with chromosome of new cell 3) Some phage accidently carry host DNA fragment 4) Phage infects new cell 5) Host DNA digested into fragments 
A)  1) Phage infects cell 2) Phage transported DNA recombines with chromosome of new cell 3) Host DNA digested into fragments 4) Some phage accidently carry host DNA fragment 5) Phage infects new cell 
B)  1) Phage infects cell 2) Phage transported DNA recombines with chromosome of new cell 3) Host DNA digested into fragments 4) Phage infects new cell 5) Some phage accidently carry host DNA fragment 
C)  1) Phage infects cell 2) Host DNA digested into fragments 3) Some phage accidently carry host DNA fragment 4) Phage infects new cell 5) Phage transported DNA recombines with chromosome of new cell 
D)  1) Phage infects cell 2) Phage transported DNA recombines with chromosome of new cell 3) Some phage accidently carry host DNA fragment 4) Phage infects new cell 5) Host DNA digested into fragments

Question 2

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68?

Accepted Answer

A)  0.68=(1 - d/2)3 
B)  d=.683 - 1 
C)  d=(1-.68)2 
A)  0.68=(1 - d/2)3 
B)  d=.683 - 1 
C)  d=(1-.68)2

Question 3

Mechanism of genetic transfer that uses competent cells.

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Question 4

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA) directly from the environment?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Question 5

The protein complex that initiates the process of conjugation following contact between two bacteria is called the _________.

Accepted Answer

A)  origin of transfer 
B)  T DNA 
C)  relaxosome 
D)  nucleoprotein 
A)  origin of transfer 
B)  T DNA 
C)  relaxosome 
D)  nucleoprotein

Question 6

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

Accepted Answer

A)  It serves as a control to calculate the rate of reversion mutations 
B)  There is no experimental purpose to performing that part of the experiment 
C)  It tests that the strains were made correctly 
D)  It tests to make sure the media was made correctly 
A)  It serves as a control to calculate the rate of reversion mutations 
B)  There is no experimental purpose to performing that part of the experiment 
C)  It tests that the strains were made correctly 
D)  It tests to make sure the media was made correctly

Question 7

Competent cells are those used in studies of conjugation.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following is correct concerning F factors?

Accepted Answer

A)  It may integrate into the host chromosome 
B)  It stands for fertility factor 
C)  It allows conjugation to occur 
D)  F+ bacteria act as donor strains in conjugation 
E)  All of the answers are correct 
A)  It may integrate into the host chromosome 
B)  It stands for fertility factor 
C)  It allows conjugation to occur 
D)  F+ bacteria act as donor strains in conjugation 
E)  All of the answers are correct

Question 9

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

Accepted Answer

A) True 
 B)False

Question 10

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

Accepted Answer

A)  Raise the cotransduction frequency 
B)  Lower the cotransduction frequency 
C)  Have no effect on the cotransduction frequency 
A)  Raise the cotransduction frequency 
B)  Lower the cotransduction frequency 
C)  Have no effect on the cotransduction frequency

Question 11

Which mechanism of genetic transfer utilizes a sex pilus?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Question 12

Complementation experiments are used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

Accepted Answer

A) True 
 B)False

Question 13

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

Accepted Answer

A) True 
 B)False

Question 14

Which statement is true concerning intragenic and intergenic gene mapping?

Accepted Answer

A)  The number of expected wild type recombinant viruses will be greater in intragenic mapping than with intergenic mapping 
B)  Intergenic gene mapping can only be performed in viruses.It is not performed in bacteria while intragenic mapping is performed in both viruses and bacteria 
C)  Intragenic mapping is the mapping of mutations within a gene while intergenic mapping is the mapping of genes with respect to each other 
D)  Intergenic mapping is the mapping of mutations within a gene while intragenic mapping is the mapping of genes with respect to each other 
A)  The number of expected wild type recombinant viruses will be greater in intragenic mapping than with intergenic mapping 
B)  Intergenic gene mapping can only be performed in viruses.It is not performed in bacteria while intragenic mapping is performed in both viruses and bacteria 
C)  Intragenic mapping is the mapping of mutations within a gene while intergenic mapping is the mapping of genes with respect to each other 
D)  Intergenic mapping is the mapping of mutations within a gene while intragenic mapping is the mapping of genes with respect to each other

Question 15

Which of the following conditions must be correct for transformation to occur?

Accepted Answer

A)  Temperature 
B)  Ionic conditions 
C)  Correct nutrient balance 
D)  All of the answers are correct 
A)  Temperature 
B)  Ionic conditions 
C)  Correct nutrient balance 
D)  All of the answers are correct

Question 16

Based on the following data, what two genes are farthest apart?

Accepted Answer

A)  a-b 
B)  b-c 
C)  a-c 
A)  a-b 
B)  b-c 
C)  a-c

Question 17

Although there are several different types of plasmids they all

Accepted Answer

A)  can confer some kind of selective growth advantage depending on environmental conditions. 
B)  can induce a mating phenotype. 
C)  result in horizontal transfer. 
D)  make cells resistant to antibiotics 
A)  can confer some kind of selective growth advantage depending on environmental conditions. 
B)  can induce a mating phenotype. 
C)  result in horizontal transfer. 
D)  make cells resistant to antibiotics

Question 18

In the experiment performed by Lederberg and Tatum bacteria of different genotypes had to be mixed together to obtain genetic transfer. However, Davis performed the experiment in a slightly different manner by separating cells with a filter incapable of allowing bacteria to pass. What possibility did the experiment by Davis exclude that could have occurred by the protocol used by Lederberg and Tatum?

Accepted Answer

A)  transformation 
B)  conjugation 
C)  generalized transduction 
D)  specialized transduction 
A)  transformation 
B)  conjugation 
C)  generalized transduction 
D)  specialized transduction

Question 19

Which of the following is true regarding conjugation?

Accepted Answer

A)  The bacteria must be in physical contact 
B)  Most bacterial species have the ability to conjugate 
C)  Strains called donor strains can transfer genetic information 
D)  Donor strains can convert non-donor strains into donor strains 
E)  All of the answers are correct 
A)  The bacteria must be in physical contact 
B)  Most bacterial species have the ability to conjugate 
C)  Strains called donor strains can transfer genetic information 
D)  Donor strains can convert non-donor strains into donor strains 
E)  All of the answers are correct

Exam 7: Genetic Transfer and Mapping in Bacteria and Bacteriophages

What is the correct order for the steps in transduction?

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68?

Mechanism of genetic transfer that uses competent cells.

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA) directly from the environment?

The protein complex that initiates the process of conjugation following contact between two bacteria is called the _________.

In the Lederberg and Tatum experiment what is the purpose of performing the experiment with the strains without adding them together?

Competent cells are those used in studies of conjugation.

Which of the following is correct concerning F factors?

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

A phage that can carry a larger piece of a bacterial chromosome would have what type of effect on cotransduction frequencies?

Which mechanism of genetic transfer utilizes a sex pilus?

Complementation experiments are used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

Cotransduction establishes the general order of genes on a bacterial chromosome, while conjugation is more specific and thus may be used to establish precise distances.

Which statement is true concerning intragenic and intergenic gene mapping?

Which of the following conditions must be correct for transformation to occur?

Based on the following data, what two genes are farthest apart?

Although there are several different types of plasmids they all

Which of the following is true regarding conjugation?

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Molecular Structure of DNA and RNA

Chromosome Organization and Molecular Structure

DNA Replication

Gene Transcription and RNA Modification

Translation of MRNA

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional Regulation

Gene Regulation in Eukaryotes II: Epigenetics and Regulation at the RNA Level

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination and Transposition at the Molecular Level

DNA Technologies

Biotechnology

Genomics I: Analysis of DNA

Genomics II: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Quantitative Genetics

Evolutionary Genetics

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