Question 1

___________________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Accepted Answer

Restriction endonucl

Question 2

What is a DNA probe?

Accepted Answer

A DNA probe is used to identify specific

Question 3

If a gene is inserted into the gene that encodes an antibiotic resistance marker,will the resulting clone be sensitive or resistant to the antibiotic?

Accepted Answer

When inserted into a gene,the

Question 4

How is a single gene of interest identified on a plate containing many different library clones?

Accepted Answer

By using a probe specific for the DNA of

Question 5

What type of vector is used to produce large amounts of a desired protein in a bacterial cell?

Accepted Answer

A)  cloning vector 
B)  replication vector 
C)  transcription vector 
D)  translation vector 
E)  expression vector 
A)  cloning vector 
B)  replication vector 
C)  transcription vector 
D)  translation vector 
E)  expression vector

Question 6

The specificity or stringency of a PCR reaction can be controlled by altering the reaction

Accepted Answer

A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  time. 
E)  None of the answers is correct. 
A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  time. 
E)  None of the answers is correct.

Question 7

Plasmids used in recombinant DNA technology typically

Accepted Answer

A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  contain reporter genes. 
E)  All of the answers are correct. 
A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  contain reporter genes. 
E)  All of the answers are correct.

Question 8

A plasmid is an example of a common ______.

Accepted Answer

A)  cosmid 
B)  chromosome 
C)  gene 
D)  vector 
E)  None of the answers is correct. 
A)  cosmid 
B)  chromosome 
C)  gene 
D)  vector 
E)  None of the answers is correct.

Question 9

What enzyme is used to create DNA from RNA?

Accepted Answer

A)  RNA polymerase 
B)  DNA polymerase 
C)  reverse transcriptase 
D)  RNA-DNA transcriptase 
E)  None of the answers is correct. 
A)  RNA polymerase 
B)  DNA polymerase 
C)  reverse transcriptase 
D)  RNA-DNA transcriptase 
E)  None of the answers is correct.

Question 10

A polylinker site contains

Accepted Answer

A)  many closely spaced restriction enzyme sites. 
B)  links for antibiotic resistance. 
C)  sequences allowing linkage to mRNA. 
D)  a sequence that allows for insertion into the host chromosome. 
E)  None of the answers is correct. 
A)  many closely spaced restriction enzyme sites. 
B)  links for antibiotic resistance. 
C)  sequences allowing linkage to mRNA. 
D)  a sequence that allows for insertion into the host chromosome. 
E)  None of the answers is correct.

Exam 5: Exploring Genes and Genomes

___________________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

What is a DNA probe?

If a gene is inserted into the gene that encodes an antibiotic resistance marker,will the resulting clone be sensitive or resistant to the antibiotic?

How is a single gene of interest identified on a plate containing many different library clones?

What type of vector is used to produce large amounts of a desired protein in a bacterial cell?

The specificity or stringency of a PCR reaction can be controlled by altering the reaction

Plasmids used in recombinant DNA technology typically

A plasmid is an example of a common ______.

What enzyme is used to create DNA from RNA?

A polylinker site contains

Biochemistry: An Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

Oxidative Phosphorylation

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, repair, and Recombination

RNA Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

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