Question 1

The probe used to isolate a gene from a genomic library is often

Accepted Answer

A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  the protein produced by the gene. 
E)  None of the answers is correct. 
A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  the protein produced by the gene. 
E)  None of the answers is correct.

Question 2

Describe two ways PCR can be used in medical diagnosis.

Accepted Answer

PCR can be used to identify the presence

Question 3

What do Southern,northern,and western blots detect,respectively?

Accepted Answer

A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA 
A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA

Question 4

What advantage can be gained by splicing together portions of two different genes?

Accepted Answer

This technique allows the creation of no

Question 5

Briefly outline the steps necessary to create a recombinant DNA molecule.

Accepted Answer

Both the fragment of interest

Question 6

Comparative genomics involves

Accepted Answer

A)  looking for similarities in complementary strands of DNA. 
B)  using sequence information to compare human DNA with that from other organisms. 
C)  comparing protein expressed in different cell types. 
D)  comparing mitochondrial versus nuclear DNA. 
E)  None of the answers is correct. 
A)  looking for similarities in complementary strands of DNA. 
B)  using sequence information to compare human DNA with that from other organisms. 
C)  comparing protein expressed in different cell types. 
D)  comparing mitochondrial versus nuclear DNA. 
E)  None of the answers is correct.

Question 7

The biological role of restriction enzymes in bacteria is to

Accepted Answer

A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  join gaps in DNA. 
E)  None of the answers is correct. 
A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  join gaps in DNA. 
E)  None of the answers is correct.

Question 8

Why are Met and Trp codons often used to design DNA probes from amino acid sequences?

Accepted Answer

A)  They are not degenerate and have single codons. 
B)  Met is the first amino acid in the protein chain. 
C)  Both are used often in proteins. 
D)  They always occur together in protein sequences. 
E)  None of the answers is correct. 
A)  They are not degenerate and have single codons. 
B)  Met is the first amino acid in the protein chain. 
C)  Both are used often in proteins. 
D)  They always occur together in protein sequences. 
E)  None of the answers is correct.

Question 9

Design a potential DNA-restriction enzyme site.Show both strands.

Accepted Answer

Any palindromic (inv

Question 10

Reverse transcriptase is normally encoded in DNA from

Accepted Answer

A)  plants. 
B)  retroviruses. 
C)  mitochondria. 
D)  archaea. 
E)  None of the answers is correct. 
A)  plants. 
B)  retroviruses. 
C)  mitochondria. 
D)  archaea. 
E)  None of the answers is correct.

Question 11

A pattern of DNA fragments can serve as a _____ of a particular DNA molecule.

Accepted Answer

A)  template 
B)  primer 
C)  fingerprint 
D)  vector 
E)  None of the answers is correct. 
A)  template 
B)  primer 
C)  fingerprint 
D)  vector 
E)  None of the answers is correct.

Question 12

Current DNA sequencing commonly uses _____ base analogues.

Accepted Answer

A)  fluorescent 
B)  radioactive 
C)  phosphorescent 
D)  cross-linked 
E)  photoreactive 
A)  fluorescent 
B)  radioactive 
C)  phosphorescent 
D)  cross-linked 
E)  photoreactive

Question 13

The first three bases of the six-base recognition cleavage site of HindIII are AAG.What is the complete sequence of this 6 bp site?

Accepted Answer

A)  AAGAAG 
B)  AAGCTT 
C)  AAGGAA 
D)  AAGCUU 
E)  None of the answers is correct. 
A)  AAGAAG 
B)  AAGCTT 
C)  AAGGAA 
D)  AAGCUU 
E)  None of the answers is correct.

Question 14

How is a gene gun used?

Accepted Answer

DNA is coated onto tungsten pe

Question 15

Explain the basis of the Sanger method.

Accepted Answer

Sanger sequencing is based upon replicat

Question 16

Genes can be inserted into eukaryotic cells by

Accepted Answer

A)  viruses. 
B)  plasmids. 
C)  microinjection. 
D)  All of the answers are correct. 
E)  None of the answers is correct. 
A)  viruses. 
B)  plasmids. 
C)  microinjection. 
D)  All of the answers are correct. 
E)  None of the answers is correct.

Question 17

The enzyme _________________ can be used to add nucleotides to the 3' end of DNA.

Accepted Answer

terminal t

Question 18

Animals that harbor a foreign gene as a result of recombinant gene manipulation are called

Accepted Answer

A)  transgenic. 
B)  mutants. 
C)  aliens. 
D)  All of the answers are correct. 
E)  None of the answers is correct. 
A)  transgenic. 
B)  mutants. 
C)  aliens. 
D)  All of the answers are correct. 
E)  None of the answers is correct.

Question 19

In solid-phase synthesis of oligonucleotides,the 2'deoxyribonucleotide-3'-phosphoramidite is added to the _______ end of the growing oligonucleotide.

Accepted Answer

The answer of In solid-phase synthesis of oligonucleotides,the 2'deoxyribonucleotide-3'-phosphoramidite is...

Question 20

Techniques for engineering new proteins by site-directed gene mutations include

Accepted Answer

A)  oligonucleotide directed mutagenesis. 
B)  cassette mutagenesis. 
C)  chromosome walking mutagenesis. 
D)  oligonucleotide directed mutagenesis and cassette mutagenesis. 
E)  All of the answers are correct. 
A)  oligonucleotide directed mutagenesis. 
B)  cassette mutagenesis. 
C)  chromosome walking mutagenesis. 
D)  oligonucleotide directed mutagenesis and cassette mutagenesis. 
E)  All of the answers are correct.

Exam 5: Exploring Genes and Genomes

The probe used to isolate a gene from a genomic library is often

Describe two ways PCR can be used in medical diagnosis.

What do Southern,northern,and western blots detect,respectively?

What advantage can be gained by splicing together portions of two different genes?

Briefly outline the steps necessary to create a recombinant DNA molecule.

Comparative genomics involves

The biological role of restriction enzymes in bacteria is to

Why are Met and Trp codons often used to design DNA probes from amino acid sequences?

Design a potential DNA-restriction enzyme site.Show both strands.

Reverse transcriptase is normally encoded in DNA from

A pattern of DNA fragments can serve as a _____ of a particular DNA molecule.

Current DNA sequencing commonly uses _____ base analogues.

The first three bases of the six-base recognition cleavage site of HindIII are AAG.What is the complete sequence of this 6 bp site?

How is a gene gun used?

Explain the basis of the Sanger method.

Genes can be inserted into eukaryotic cells by

The enzyme _________________ can be used to add nucleotides to the 3' end of DNA.

Animals that harbor a foreign gene as a result of recombinant gene manipulation are called

In solid-phase synthesis of oligonucleotides,the 2'deoxyribonucleotide-3'-phosphoramidite is added to the _______ end of the growing oligonucleotide.

Techniques for engineering new proteins by site-directed gene mutations include

Biochemistry: An Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

Oxidative Phosphorylation

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, repair, and Recombination

RNA Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

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