Question 1

In an agglutination reaction,the antibody isotype that most effectively overcomes the electrostatic repulsion between particles is:

Accepted Answer

A)  IgA. 
B)  IgE. 
C)  IgG. 
D)  IgM. 
A)  IgA. 
B)  IgE. 
C)  IgG. 
D)  IgM.

Question 2

In performing blood typing using the tube method,if the red cell button is not resuspended properly,what are the most likely results?

Accepted Answer

A)  False positive 
B)  False negative 
C)  Does not affect the results 
D)  Makes the reaction weaker 
A)  False positive 
B)  False negative 
C)  Does not affect the results 
D)  Makes the reaction weaker

Question 3

Which antibody has the highest avidity for an antigen?

Accepted Answer

A)  IgG containing Fab that each have an affinity of 4 × 10-?/M 
B)  Secretory IgA containing Fab that each have an affinity of 4 × 10-?/M 
C)  IgM containing Fab that each have an affinity of 1 × 10-?/M 
D)  IgE containing Fab that each have an affinity of 1 × 10-? 
A)  IgG containing Fab that each have an affinity of 4 × 10-?/M 
B)  Secretory IgA containing Fab that each have an affinity of 4 × 10-?/M 
C)  IgM containing Fab that each have an affinity of 1 × 10-?/M 
D)  IgE containing Fab that each have an affinity of 1 × 10-?

Question 4

A patient's serum sample is added to a solution containing particulate antigen,but no agglutination is observed.What should be done next?

Accepted Answer

A)  Perform serial dilutions of the sample. 
B)  Report out as a negative test. 
C)  Add more particulate antigen. 
D)  Read the reaction in dim light. 
A)  Perform serial dilutions of the sample. 
B)  Report out as a negative test. 
C)  Add more particulate antigen. 
D)  Read the reaction in dim light.

Question 5

The particle-counting immunoassay method counts the number of:

Accepted Answer

A)  agglutinated particles. 
B)  free antibody molecules. 
C)  free latex particles. 
D)  antigen-antibody bonds. 
A)  agglutinated particles. 
B)  free antibody molecules. 
C)  free latex particles. 
D)  antigen-antibody bonds.

Question 6

Agglutination reactions run at room temperature are appropriate for antigen-antibody reactions involving:

Accepted Answer

A)  IgA. 
B)  IgE. 
C)  IgG. 
D)  IgM. 
A)  IgA. 
B)  IgE. 
C)  IgG. 
D)  IgM.

Question 7

Based on the following equilibrium constants,which assay would have the best sensitivity?

Accepted Answer

A)  K = 1 
B)  K = 2 
C)  K = 4 
D)  K = 9 
A)  K = 1 
B)  K = 2 
C)  K = 4 
D)  K = 9

Question 8

A solution has 4 pentomeric IgM antibodies per microliter.What concentration of antigen molecules is necessary for the solution to be in the zone of equivalence? The antigen has 4 identical epitopes on each molecule.

Accepted Answer

A)  4 antigen molecules per microliter 
B)  5 antigen molecules per microliter 
C)  10 antigen molecules per microliter 
D)  20 antigen molecules per microliter 
A)  4 antigen molecules per microliter 
B)  5 antigen molecules per microliter 
C)  10 antigen molecules per microliter 
D)  20 antigen molecules per microliter

Question 9

If crossed lines result in an Ouchterlony immunodiffusion reaction with antigens A and B,this indicates that antigens A and B:

Accepted Answer

A)  are identical. 
B)  are entirely different. 
C)  share a common epitope, with A being a more complex antigen. 
D)  share a common epitope, with B being a more complex antigen. 
A)  are identical. 
B)  are entirely different. 
C)  share a common epitope, with A being a more complex antigen. 
D)  share a common epitope, with B being a more complex antigen.

Question 10

If a serum sample does not cause agglutination when mixed with a particular antigen,then you should:

Accepted Answer

A)  report that the patient does not have antibody against that antigen. 
B)  test a more diluted sample to overcome possible prozone effects. 
C)  increase the particle surface charge by altering the pH. 
D)  decrease the viscosity of the sample. 
A)  report that the patient does not have antibody against that antigen. 
B)  test a more diluted sample to overcome possible prozone effects. 
C)  increase the particle surface charge by altering the pH. 
D)  decrease the viscosity of the sample.

Question 11

A radial immunodiffusion test is run for 72 hours.The concentration of the antigen is then calculated using the:

Accepted Answer

A)  radius of the precipitation ring. 
B)  diameter of the precipitation ring. 
C)  square of the diameter of the precipitation ring. 
D)  log of the diameter of the precipitation ring. 
A)  radius of the precipitation ring. 
B)  diameter of the precipitation ring. 
C)  square of the diameter of the precipitation ring. 
D)  log of the diameter of the precipitation ring.

Question 12

Which best describes hemagglutination tests?

Accepted Answer

A)  Patient antibody reacts with hemoglobin. 
B)  The reaction involves red blood cells. 
C)  Patient antibody is always involved. 
D)  Hemoglobin binds to antigen. 
A)  Patient antibody reacts with hemoglobin. 
B)  The reaction involves red blood cells. 
C)  Patient antibody is always involved. 
D)  Hemoglobin binds to antigen.

Question 13

In immunofixation electrophoresis,antibody is:

Accepted Answer

A)  distributed uniformly throughout the gel. 
B)  applied on the top surface of the gel. 
C)  added to troughs cut into the gel. 
D)  driven through the gel by application of electrical current. 
A)  distributed uniformly throughout the gel. 
B)  applied on the top surface of the gel. 
C)  added to troughs cut into the gel. 
D)  driven through the gel by application of electrical current.

Question 14

A serological test that uses red blood cells coated with exogenous antigens such as bacterial polysaccharides as a method to detect patient antibodies against those exogenous antigens is called:

Accepted Answer

A)  latex agglutination. 
B)  hemagglutination. 
C)  neutralization. 
D)  complement fixation. 
A)  latex agglutination. 
B)  hemagglutination. 
C)  neutralization. 
D)  complement fixation.

Question 15

When carrier particles are coated with an antigen that is not normally found on them,this is known as:

Accepted Answer

A)  direct agglutination. 
B)  passive agglutination. 
C)  reverse passive agglutination. 
D)  hemagglutination. 
A)  direct agglutination. 
B)  passive agglutination. 
C)  reverse passive agglutination. 
D)  hemagglutination.

In an agglutination reaction,the antibody isotype that most effectively overcomes the electrostatic repulsion between particles is:

In performing blood typing using the tube method,if the red cell button is not resuspended properly,what are the most likely results?

Which antibody has the highest avidity for an antigen?

A patient's serum sample is added to a solution containing particulate antigen,but no agglutination is observed.What should be done next?

The particle-counting immunoassay method counts the number of:

Agglutination reactions run at room temperature are appropriate for antigen-antibody reactions involving:

Based on the following equilibrium constants,which assay would have the best sensitivity?

A solution has 4 pentomeric IgM antibodies per microliter.What concentration of antigen molecules is necessary for the solution to be in the zone of equivalence? The antigen has 4 identical epitopes on each molecule.

If crossed lines result in an Ouchterlony immunodiffusion reaction with antigens A and B,this indicates that antigens A and B:

If a serum sample does not cause agglutination when mixed with a particular antigen,then you should:

A radial immunodiffusion test is run for 72 hours.The concentration of the antigen is then calculated using the:

Which best describes hemagglutination tests?

In immunofixation electrophoresis,antibody is:

A serological test that uses red blood cells coated with exogenous antigens such as bacterial polysaccharides as a method to detect patient antibodies against those exogenous antigens is called:

When carrier particles are coated with an antigen that is not normally found on them,this is known as:

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Labeled Immunoassays

Molecular Diagnostic Techniques

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

Filters

Exam 10: Precipitation and Agglutination Reactions

In an agglutination reaction,the antibody isotype that most effectively overcomes the electrostatic repulsion between particles is:

In performing blood typing using the tube method,if the red cell button is not resuspended properly,what are the most likely results?

Which antibody has the highest avidity for an antigen?

A patient's serum sample is added to a solution containing particulate antigen,but no agglutination is observed.What should be done next?

The particle-counting immunoassay method counts the number of:

Agglutination reactions run at room temperature are appropriate for antigen-antibody reactions involving:

Based on the following equilibrium constants,which assay would have the best sensitivity?

A solution has 4 pentomeric IgM antibodies per microliter.What concentration of antigen molecules is necessary for the solution to be in the zone of equivalence? The antigen has 4 identical epitopes on each molecule.

If crossed lines result in an Ouchterlony immunodiffusion reaction with antigens A and B,this indicates that antigens A and B:

If a serum sample does not cause agglutination when mixed with a particular antigen,then you should:

A radial immunodiffusion test is run for 72 hours.The concentration of the antigen is then calculated using the:

Which best describes hemagglutination tests?

In immunofixation electrophoresis,antibody is:

A serological test that uses red blood cells coated with exogenous antigens such as bacterial polysaccharides as a method to detect patient antibodies against those exogenous antigens is called:

When carrier particles are coated with an antigen that is not normally found on them,this is known as:

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Labeled Immunoassays

Molecular Diagnostic Techniques

Flow Cytometry and Laboratory Automation

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

Filters