Question 1

As measured in flow cytometry,cells that are the smallest and have the least granules would be identified as:

Accepted Answer

A)  lymphocytes. 
B)  granulocytes. 
C)  monocytes. 
D)  erythrocytes. 
A)  lymphocytes. 
B)  granulocytes. 
C)  monocytes. 
D)  erythrocytes.

Question 2

In flow cytometry,the sheath fluid:

Accepted Answer

A)  contains a dye required for cell identification. 
B)  draws the suspended cells into a laminar flow. 
C)  filters out extraneous wavelengths of light. 
D)  provides nutrients necessary to maintain cellular metabolism. 
A)  contains a dye required for cell identification. 
B)  draws the suspended cells into a laminar flow. 
C)  filters out extraneous wavelengths of light. 
D)  provides nutrients necessary to maintain cellular metabolism.

Question 3

What is meant by "gating" in flow cytometry?

Accepted Answer

A)  The chromatic arrangement of cellular populations 
B)  The process by which one cell population adheres to another population 
C)  An electronic window separating subpopulations of cells 
D)  An electronic device measuring all populations of cells 
A)  The chromatic arrangement of cellular populations 
B)  The process by which one cell population adheres to another population 
C)  An electronic window separating subpopulations of cells 
D)  An electronic device measuring all populations of cells

Question 4

A mature cytotoxic T cell expresses which markers?

Accepted Answer

A)  CD3, CD4, and CD8 
B)  CD4, CD3, and CD5 
C)  CD5, CD8, and CD3 
D)  CD8, CD3, and CD2 
A)  CD3, CD4, and CD8 
B)  CD4, CD3, and CD5 
C)  CD5, CD8, and CD3 
D)  CD8, CD3, and CD2

Question 5

A test system is producing many false-positive results.You suspect this is a problem involving:

Accepted Answer

A)  analytic specificity. 
B)  precision. 
C)  analytic sensitivity. 
D)  a sampling error. 
A)  analytic specificity. 
B)  precision. 
C)  analytic sensitivity. 
D)  a sampling error.

Question 6

A fluorescent signal in flow cytometry is generated by:

Accepted Answer

A)  light reflected off cells passing through a laser beam. 
B)  light scattered by fluorochromes. 
C)  fluorochromes that absorb energy from a laser beam and then release the energy as longer-wavelength light. 
D)  cell metabolism. 
A)  light reflected off cells passing through a laser beam. 
B)  light scattered by fluorochromes. 
C)  fluorochromes that absorb energy from a laser beam and then release the energy as longer-wavelength light. 
D)  cell metabolism.

Question 7

In flow cytometry,forward scatter provides a measure of:

Accepted Answer

A)  cell size. 
B)  cell granularity. 
C)  membrane receptor expression. 
A)  cell size. 
B)  cell granularity. 
C)  membrane receptor expression.

As measured in flow cytometry,cells that are the smallest and have the least granules would be identified as:

In flow cytometry,the sheath fluid:

What is meant by "gating" in flow cytometry?

A mature cytotoxic T cell expresses which markers?

A test system is producing many false-positive results.You suspect this is a problem involving:

A fluorescent signal in flow cytometry is generated by:

In flow cytometry,forward scatter provides a measure of:

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Precipitation and Agglutination Reactions

Labeled Immunoassays

Molecular Diagnostic Techniques

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

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Exam 13: Flow Cytometry and Laboratory Automation

As measured in flow cytometry,cells that are the smallest and have the least granules would be identified as:

In flow cytometry,the sheath fluid:

What is meant by "gating" in flow cytometry?

A mature cytotoxic T cell expresses which markers?

A test system is producing many false-positive results.You suspect this is a problem involving:

A fluorescent signal in flow cytometry is generated by:

In flow cytometry,forward scatter provides a measure of:

Introduction to Immunity and the Immune System

Nature of Antigens and the Major Histocompatibility Complex

Innate Immunity

Adaptive Immunity

Antibody Structure and Function

Cytokines

Complement System

Safety and Quality Management

Principles of Serological Testing

Precipitation and Agglutination Reactions

Labeled Immunoassays

Molecular Diagnostic Techniques

Hypersensitivity

Autoimmunity

Transplantation Immunology

Tumor Immunology

Immunoproliferative Diseases

Immunodeficiency Diseases

Serological and Molecular Detection of Bacterial Infections

Spirochete Diseases

Serological and Molecular Diagnosis of Parasitic and Fungal Infections

Serology and Molecular Detection of Viral Infections

Laboratory Diagnosis of HIV Infection

Immunization and Vaccines

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