Exam 23: Genomics I: Analysis of Dna

In positional cloning, which method is used to identify clones that are progressively closer to a gene of interest?

Cytogenetic mapping is accurate to within 500,000 base pairs, but not over this value.

Map units correspond to fixed physical distances.

The process of in situ hybridization is used for which of the following?

Shotgun sequencing is precise enough to achieve a complete genomic sequence without the need for other mapping methods.

YAC stands for yeast artificial cosmid.

The human genome project's first draft took approximately ________ years.

Which of the following is the most direct benefit of high-throughput Sanger sequencing?

Match each type of chromosome mapping with the units used to measure the distance between loci. A) Number of base pairs (bp) B) Map units (mu) C) Distance between bands -Cytogenetic mapping

The method of sequencing genomic fragments that are randomly generated from larger DNA fragments is called

The human genome consists of approximately ________ base pairs of DNA.

Which of the following procedures best determines the relative order, but not precise location, of a series of genes on a chromosome?

A series of clones that contain overlapping pieces of a chromosome are called a ________.

For this question, match each molecular marker with its correct description. Each answer is used only once. A) RFLP B) SNP C) STR D) STS -A site where a single nucleotide is variable between individuals

For this question, match each molecular marker with its correct description. Each answer is used only once. A) RFLP B) SNP C) STR D) STS -A microsatellite, contains repeat sequences that are typically between 50 and 200 base pairs in length

In sequencing by synthesis (SBS) methods, the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

Metagenomics is a common approach for studying the genes of a clonal population of bacteria in the laboratory.