Question 1

Okazaki fragments do which of the following?

Accepted Answer

A)  Assist in forming the replication fork 
B)  Bind to the oriC region 
C)  Assist in the synthesis of DNA from the lagging strand 
D)  Reform the double-helix following replication 
E)  None of the answers are correct 
A)  Assist in forming the replication fork 
B)  Bind to the oriC region 
C)  Assist in the synthesis of DNA from the lagging strand 
D)  Reform the double-helix following replication 
E)  None of the answers are correct

Question 2

Fills in small regions of DNA where the RNA primers were located.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 3

DNA polymerase III has an error in replication once every 100 million nucleotides.

Accepted Answer

A) True 
 B)False

Question 4

The origin of replication in bacteria is called OriC.

Accepted Answer

A) True 
 B)False

Question 5

You have discovered a strain of E. coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes. Upon further investigation, you find a mutation in the DNA polymerase III gene. What subunit of the holoenzyme does this mutation affect the most?

Accepted Answer

A)  α 
B)  β 
C)  ε 
D)  δ 
A)  α 
B)  β 
C)  ε 
D)  δ

Question 6

DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

Accepted Answer

A) True 
 B)False

Question 7

The synthesis of the daughter strand of DNA occurs away from the replication fork in the leading strand.

Accepted Answer

A) True 
 B)False

Question 8

The Meselson-Stahl experiments used 35S radioisotopes to determine the mechanism of DNA replication.

Accepted Answer

A) True 
 B)False

Question 9

What functions are accomplished by the primosome?

Accepted Answer

A)  Tracking along DNA 
B)  Tracking along DNA, separating double stranded DNA 
C)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers 
D)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers, adding nucleotides 
A)  Tracking along DNA 
B)  Tracking along DNA, separating double stranded DNA 
C)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers 
D)  Tracking along DNA, separating double stranded DNA, synthesizing RNA primers, adding nucleotides

Question 10

The purpose of DNA replication is to produce ________.

Accepted Answer

A)  Two daughter strands 
B)  Two parental strands 
C)  Two template strands 
D)  None of the answers are correct 
A)  Two daughter strands 
B)  Two parental strands 
C)  Two template strands 
D)  None of the answers are correct

Question 11

The movement of the replication fork in bacterial replication is unidirectional.

Accepted Answer

A) True 
 B)False

Question 12

In eukaryotes, which of the following is similar to the oriC region of prokaryotes?

Accepted Answer

A)  Dam 
B)  ARS elements 
C)  Promoters 
D)  Telomeres 
A)  Dam 
B)  ARS elements 
C)  Promoters 
D)  Telomeres

Question 13

Replication usually begins in GC rich regions due to the presence of only 2 hydrogen bonds between the bases.

Accepted Answer

A) True 
 B)False

Question 14

Removes supercoiling ahead of the replication fork.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 15

If eukaryotic cells evolved such that lagging strand DNA synthesis could occur continuously (without the use of Okazaki fragments), what enzyme would likely no longer be needed for DNA replication?

Accepted Answer

A)  Flap endonuclease 
B)  Helicase 
C)  Primase 
D)  Topoisomerase 
A)  Flap endonuclease 
B)  Helicase 
C)  Primase 
D)  Topoisomerase

Question 16

Which of the following is not correct concerning the initiation of bacterial replication?

Accepted Answer

A)  It involves a region of the DNA called oriC 
B)  DnaA proteins bind to the DNA to begin separation of the strands 
C)  The strands are initially separated at GC-rich regions of DNA 
D)  Following initial separation, DNA helicase enzymes continue to unwrap the DNA 
A)  It involves a region of the DNA called oriC 
B)  DnaA proteins bind to the DNA to begin separation of the strands 
C)  The strands are initially separated at GC-rich regions of DNA 
D)  Following initial separation, DNA helicase enzymes continue to unwrap the DNA

Question 17

Which of the following is an example of a processive enzyme?

Accepted Answer

A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  Okazaki fragments 
A)  DNA polymerase I 
B)  DNA polymerase III 
C)  DNA ligase 
D)  Okazaki fragments

Question 18

Synthesizes the lagging strand of the DNA.

Accepted Answer

A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III 
A)  DNA ligase 
B)  DNA primase 
C)  Topoisomerase 
D)  DNA polymerase I 
E)  DNA polymerase III

Question 19

You extract DNA from an E. coli cell and observe it is hemimethylated (only methylated on one strand). Which strand of DNA is older?

Accepted Answer

A)  The methylated strand 
B)  The strand that is not methylated 
C)  Neither-they are the same "age." 
A)  The methylated strand 
B)  The strand that is not methylated 
C)  Neither-they are the same "age."

Question 20

DNA polymerases add new nucleotides in what direction?

Accepted Answer

A)  5' to 3' 
B)  3 ' to 5' 
C)  Both directions 
A)  5' to 3' 
B)  3 ' to 5' 
C)  Both directions

Exam 11: Dna Replication

Okazaki fragments do which of the following?

Fills in small regions of DNA where the RNA primers were located.

DNA polymerase III has an error in replication once every 100 million nucleotides.

The origin of replication in bacteria is called OriC.

You have discovered a strain of E. coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes. Upon further investigation, you find a mutation in the DNA polymerase III gene. What subunit of the holoenzyme does this mutation affect the most?

DNA polymerase III has the ability to begin synthesis of the new daughter strands immediately following the formation of the replication fork.

The synthesis of the daughter strand of DNA occurs away from the replication fork in the leading strand.

The Meselson-Stahl experiments used 35S radioisotopes to determine the mechanism of DNA replication.

What functions are accomplished by the primosome?

The purpose of DNA replication is to produce ________.

The movement of the replication fork in bacterial replication is unidirectional.

In eukaryotes, which of the following is similar to the oriC region of prokaryotes?

Replication usually begins in GC rich regions due to the presence of only 2 hydrogen bonds between the bases.

Removes supercoiling ahead of the replication fork.

If eukaryotic cells evolved such that lagging strand DNA synthesis could occur continuously (without the use of Okazaki fragments), what enzyme would likely no longer be needed for DNA replication?

Which of the following is not correct concerning the initiation of bacterial replication?

Which of the following is an example of a processive enzyme?

Synthesizes the lagging strand of the DNA.

You extract DNA from an E. coli cell and observe it is hemimethylated (only methylated on one strand). Which strand of DNA is older?

DNA polymerases add new nucleotides in what direction?

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Genetic Transfer and Mapping in Bacteria

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional and Translation Regulation

Gene Regulation in Eukaryotes Ii: Epigenetics

Non-Coding Rnas

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination, Immunogenetics, and Transposition

Molecular Technologies

Biotechnology

Genomics I: Analysis of Dna

Genomics Ii: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Complex and Quantitative Traits

Evolutionary Genetics

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