Question 1

What is the maximum size of the E. coli chromosome capable of being carried by a P1 phage?

Accepted Answer

A)  10%-20% 
B)  30% 
C)  0.5%-1% 
D)  2%-2.5% 
A)  10%-20% 
B)  30% 
C)  0.5%-1% 
D)  2%-2.5%

Question 2

What is the correct order for the steps in transduction?

Accepted Answer

A)  (1) Phage infects cell. (2) Phage transported DNA recombines with chromosome of new cell. (3) Host DNA digested into fragments. (4) Some phage accidentally carry host DNA fragment. (5) Phage infects new cell. 
B)  (1) Phage infects cell. (2) Phage transported DNA recombines with chromosome of new cell. (3) Host DNA digested into fragments. (4) Phage infects new cell. (5) Some phage accidentally carry host DNA fragment. 
C)  (1) Phage infects cell. (2) Host DNA digested into fragments. (3) Some phage accidentally carry host DNA fragment. (4) Phage infects new cell. (5) Phage transported DNA recombines with chromosome of new cell. 
D)  (1) Phage infects cell. (2) Phage transported DNA recombines with chromosome of new cell. (3) Some phage accidentally carry host DNA fragment. (4) Phage infects new cell. (5) Host DNA digested into fragments. 
A)  (1) Phage infects cell. (2) Phage transported DNA recombines with chromosome of new cell. (3) Host DNA digested into fragments. (4) Some phage accidentally carry host DNA fragment. (5) Phage infects new cell. 
B)  (1) Phage infects cell. (2) Phage transported DNA recombines with chromosome of new cell. (3) Host DNA digested into fragments. (4) Phage infects new cell. (5) Some phage accidentally carry host DNA fragment. 
C)  (1) Phage infects cell. (2) Host DNA digested into fragments. (3) Some phage accidentally carry host DNA fragment. (4) Phage infects new cell. (5) Phage transported DNA recombines with chromosome of new cell. 
D)  (1) Phage infects cell. (2) Phage transported DNA recombines with chromosome of new cell. (3) Some phage accidentally carry host DNA fragment. (4) Phage infects new cell. (5) Host DNA digested into fragments.

Question 3

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68, in a P1 transduction?

Accepted Answer

A)  0.68=(1 - d/2)3 
B)  d=.683 - 1 
C)  d=(1-.68)2 
A)  0.68=(1 - d/2)3 
B)  d=.683 - 1 
C)  d=(1-.68)2

Question 4

Based on the following data, what two genes are farthest apart?

Accepted Answer

A)  a-b 
B)  b-c 
C)  a-c 
A)  a-b 
B)  b-c 
C)  a-c

Question 5

Which of the following is correct concerning F factors?

Accepted Answer

A)  It may integrate into the host chromosome. 
B)  It stands for fertility factor. 
C)  It allows conjugation to occur. 
D)  F+ bacteria act as donor strains in conjugation. 
E)  All of the answers are correct. 
A)  It may integrate into the host chromosome. 
B)  It stands for fertility factor. 
C)  It allows conjugation to occur. 
D)  F+ bacteria act as donor strains in conjugation. 
E)  All of the answers are correct.

Question 6

The use of bacteriophages to map bacterial genes is called ________.

Accepted Answer

A)  cotransduction 
B)  transformation 
C)  conjugation 
D)  none of the answers are correct 
A)  cotransduction 
B)  transformation 
C)  conjugation 
D)  none of the answers are correct

Question 7

Competence factors do which of the following?

Accepted Answer

A)  Allow the formation of a sex pilus. 
B)  Promote bacteriophages binding for transduction. 
C)  Allow DNA in the environment to bind to the cell surface. 
D)  Inhibit genetic transfer between donor and recipient cells. 
A)  Allow the formation of a sex pilus. 
B)  Promote bacteriophages binding for transduction. 
C)  Allow DNA in the environment to bind to the cell surface. 
D)  Inhibit genetic transfer between donor and recipient cells.

Question 8

The results below are from an interrupted conjugation experiment performed with an Hfr strain. Which genes could also be mapped with transduction?

Accepted Answer

A)  Only argR and argG could be mapped to each other using transduction. 
B)  All three could be mapped to each other using transduction. 
C)  argR could be mapped to recA and argG could be mapped to recA but argR and argG are too close to each other to be mapped using transduction. 
D)  Only argG could be mapped with recA since argR is farther away from recA there would be little recombination that would include all three genes together and so argR could not be reliably mapped using transduction. 
A)  Only argR and argG could be mapped to each other using transduction. 
B)  All three could be mapped to each other using transduction. 
C)  argR could be mapped to recA and argG could be mapped to recA but argR and argG are too close to each other to be mapped using transduction. 
D)  Only argG could be mapped with recA since argR is farther away from recA there would be little recombination that would include all three genes together and so argR could not be reliably mapped using transduction.

Question 9

During conjugation, one strand of the F factor DNA remains in the donor cell and one strand is transferred to the recipient cell.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following conditions must be correct for transformation to occur?

Accepted Answer

A)  Temperature 
B)  Ionic conditions 
C)  Correct nutrient balance 
D)  All of the answers are correct 
A)  Temperature 
B)  Ionic conditions 
C)  Correct nutrient balance 
D)  All of the answers are correct

Question 11

The transfer of genetic material from two unrelated species is called ________.

Accepted Answer

A)  vertical gene transfer 
B)  conjugation 
C)  sexual reproduction 
D)  binary fission 
E)  horizontal gene transfer 
A)  vertical gene transfer 
B)  conjugation 
C)  sexual reproduction 
D)  binary fission 
E)  horizontal gene transfer

Question 12

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA) directly from the environment?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Question 13

An Hfr strain is produced when

Accepted Answer

A)  an F episome integrates into the E. coli chromosome. 
B)  an F episome leaves the E. coli chromosome. 
C)  an F episome is transferred to a new cell. 
D)  an F episome leaves the host chromosome and removes some of the host chromosome. 
A)  an F episome integrates into the E. coli chromosome. 
B)  an F episome leaves the E. coli chromosome. 
C)  an F episome is transferred to a new cell. 
D)  an F episome leaves the host chromosome and removes some of the host chromosome.

Question 14

Which mechanism of genetic transfer utilizes a sex pilus?

Accepted Answer

A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct 
A)  Transformation 
B)  Transduction 
C)  Conjugation 
D)  All of the answers are correct

Question 15

The protein complex that initiates the process of conjugation following contact between two bacteria is called the ________.

Accepted Answer

A)  origin of transfer 
B)  T DNA 
C)  relaxosome 
D)  nucleoprotein 
A)  origin of transfer 
B)  T DNA 
C)  relaxosome 
D)  nucleoprotein

Question 16

Competent cells are those used in studies of conjugation.

Accepted Answer

A) True 
 B)False

Question 17

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

Accepted Answer

A) True 
 B)False

Question 18

In the experiment performed by Lederberg and Tatum bacteria of different genotypes had to be mixed together to obtain genetic transfer. However, Davis performed the experiment in a slightly different manner by separating cells with a filter incapable of allowing bacteria to pass. What possibility did the experiment by Davis exclude that could have occurred by the protocol used by Lederberg and Tatum?

Accepted Answer

A)  Transformation 
B)  Conjugation 
C)  Transduction 
A)  Transformation 
B)  Conjugation 
C)  Transduction

Question 19

Although there are several different types of plasmids they all

Accepted Answer

A)  can confer some kind of selective growth advantage depending on environmental conditions. 
B)  can induce a mating phenotype. 
C)  result in horizontal transfer. 
D)  make cells resistant to antibiotics 
A)  can confer some kind of selective growth advantage depending on environmental conditions. 
B)  can induce a mating phenotype. 
C)  result in horizontal transfer. 
D)  make cells resistant to antibiotics

Question 20

In conjugation the DNA that will be transferred from the donor cell to the recipient cell is

Accepted Answer

A)  single-stranded. 
B)  double-stranded. 
C)  triple-stranded. 
D)  a mixture of single and double stranded. 
A)  single-stranded. 
B)  double-stranded. 
C)  triple-stranded. 
D)  a mixture of single and double stranded.

Exam 7: Genetic Transfer and Mapping in Bacteria

What is the maximum size of the E. coli chromosome capable of being carried by a P1 phage?

What is the correct order for the steps in transduction?

What is the equation that would be used to determine the distance between two genes with a cotransduction frequency of 0.68, in a P1 transduction?

Based on the following data, what two genes are farthest apart?

Which of the following is correct concerning F factors?

The use of bacteriophages to map bacterial genes is called ________.

Competence factors do which of the following?

The results below are from an interrupted conjugation experiment performed with an Hfr strain. Which genes could also be mapped with transduction?

During conjugation, one strand of the F factor DNA remains in the donor cell and one strand is transferred to the recipient cell.

Which of the following conditions must be correct for transformation to occur?

The transfer of genetic material from two unrelated species is called ________.

Which of the following mechanisms refer to when a bacteria assimilates genetic material (DNA) directly from the environment?

An Hfr strain is produced when

Which mechanism of genetic transfer utilizes a sex pilus?

The protein complex that initiates the process of conjugation following contact between two bacteria is called the ________.

Competent cells are those used in studies of conjugation.

An episome is a segment of bacterial DNA that can exist as a plasmid or be integrated into the bacterial chromosome.

Although there are several different types of plasmids they all

In conjugation the DNA that will be transferred from the donor cell to the recipient cell is

Overview of Genetics

Mendelian Inheritance

Chromosome Transmission During Cell Division and Sexual Reproduction

Extensions of Mendelian Inheritance

Non-Mendelian Inheritance

Genetic Linkage and Mapping in Eukaryotes

Variation in Chromosome Structure and Number

Molecular Structure of Dna and Rna

Chromosome Organization and Molecular Structure

Dna Replication

Gene Transcription and Rna Modification

Translation of Mrna

Gene Regulation in Bacteria

Gene Regulation in Eukaryotes I: Transcriptional and Translation Regulation

Gene Regulation in Eukaryotes Ii: Epigenetics

Non-Coding Rnas

Genetics of Viruses

Gene Mutation and Dna Repair

Recombination, Immunogenetics, and Transposition

Molecular Technologies

Biotechnology

Genomics I: Analysis of Dna

Genomics Ii: Functional Genomics, Proteomics, and Bioinformatics

Medical Genetics and Cancer

Developmental Genetics

Population Genetics

Complex and Quantitative Traits

Evolutionary Genetics

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