Question 1

The Southern blotting technique requires:

Accepted Answer

A) complementarity between the nucleotide sequence of the probe DNA and the DNA to be detected. 
B) DNA primers. 
C) complementarity between the protein being expressed and the radioactive probe. 
D) separation of proteins by size. 
E) None of the other answer options is correct. 
A) complementarity between the nucleotide sequence of the probe DNA and the DNA to be detected. 
B) DNA primers. 
C) complementarity between the protein being expressed and the radioactive probe. 
D) separation of proteins by size. 
E) None of the other answer options is correct.

Question 2

An experiment in DNA editing using CRISPR is carried out in which the editing template DNA contains a G-A mismatch in the region to be edited. At the same nucleotide site in the resulting edited targeted DNA, the base pair is expected to be:

Accepted Answer

A) G-A. 
B) G-C. 
C) T-A. 
D) C-T. 
E) None of the answer options is correct. 
A) G-A. 
B) G-C. 
C) T-A. 
D) C-T. 
E) None of the answer options is correct.

Question 3

Which of the following would block the process of DNA synthesis by DNA polymerase?

Accepted Answer

A) removal of the 3F1F1F1S1F1F1F10-OH from the last nucleotide of the daughter strand 
B) removal of the 5F1F1F1S1F1F1F10 triphosphate from the nucleotides used by DNA polymerase 
C) removal of the base from the nucleotides used by DNA polymerase 
D) removal of DNA ligase from the DNA replication process 
A) removal of the 3F1F1F1S1F1F1F10-OH from the last nucleotide of the daughter strand 
B) removal of the 5F1F1F1S1F1F1F10 triphosphate from the nucleotides used by DNA polymerase 
C) removal of the base from the nucleotides used by DNA polymerase 
D) removal of DNA ligase from the DNA replication process

Question 4

What would happen if telomerase stopped working in a cell in which it normally functions at high levels?

Accepted Answer

A) The cells would be able to divide indefinitely. 
B) Cancer would be the result. 
C) The cell would die immediately. 
D) The cell would eventually die as the DNA continued to shorten. 
E) None of the other answer options is correct. 
A) The cells would be able to divide indefinitely. 
B) Cancer would be the result. 
C) The cell would die immediately. 
D) The cell would eventually die as the DNA continued to shorten. 
E) None of the other answer options is correct.

Question 5

Given that the rate of DNA synthesis of a plasmid in yeast is 50 nucleotides per second and the circular plasmid replicates in 20 minutes, what is the approximate maximum size of the plasmid?

Accepted Answer

A) 30,000 bp 
B) 60,000 bp 
C) 120,000 bp 
A) 30,000 bp 
B) 60,000 bp 
C) 120,000 bp

Question 6

Consider a cell in which one of the proteins or enzymes involved in DNA replication is altered in a way that results in an increased rate of single-base changes in the newly synthesized DNA strand. Which function of which protein is MOST likely disrupted in this situation?

Accepted Answer

A) the unwinding function of helicase 
B) the winding stress relief function of topoisomerase II 
C) the fragment joining function of DNA ligase 
D) the proofreading function of DNA polymerase 
E) the strand separation function of single-stranded binding protein 
A) the unwinding function of helicase 
B) the winding stress relief function of topoisomerase II 
C) the fragment joining function of DNA ligase 
D) the proofreading function of DNA polymerase 
E) the strand separation function of single-stranded binding protein

Question 7

You run a PCR reaction for five cycles starting with a single DNA duplex. Theoretically, how many copies of your sequence would you now have?

Accepted Answer

A) 8 
B) 12 
C) 16 
D) 24 
E) 32 
A) 8 
B) 12 
C) 16 
D) 24 
E) 32

Question 8

Which of the following make(s) the Southern blot technique possible?

Accepted Answer

A) New nucleotides are added only to the 3' end of a growing DNA strand. 
B) A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. 
C) Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. 
D) Duplex nucleic acid molecules can be separated by size by means of electrophoresis. 
E) Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper. 
A) New nucleotides are added only to the 3' end of a growing DNA strand. 
B) A DNA strand whose 3' end terminates in a dideoxynucleotide cannot be elongated. 
C) Complementary single-stranded nucleic acid sequences can come together to form a duplex molecule. 
D) Duplex nucleic acid molecules can be separated by size by means of electrophoresis. 
E) Single-stranded nucleic acid molecules can be immobilized on certain types of filter paper.

Question 9

A DNA molecule is cut with two different restriction enzymes known to cleave it only once each. After gel electrophoresis, three different DNA fragments are detected. This result means that the original DNA molecule was:

Accepted Answer

A) circular. 
B) linear. 
C) It is not possible to make this determination from the information provided. 
A) circular. 
B) linear. 
C) It is not possible to make this determination from the information provided.

Exam 12: Dna Replication and Manipulation

The Southern blotting technique requires:

An experiment in DNA editing using CRISPR is carried out in which the editing template DNA contains a G-A mismatch in the region to be edited. At the same nucleotide site in the resulting edited targeted DNA, the base pair is expected to be:

Which of the following would block the process of DNA synthesis by DNA polymerase?

What would happen if telomerase stopped working in a cell in which it normally functions at high levels?

Given that the rate of DNA synthesis of a plasmid in yeast is 50 nucleotides per second and the circular plasmid replicates in 20 minutes, what is the approximate maximum size of the plasmid?

Consider a cell in which one of the proteins or enzymes involved in DNA replication is altered in a way that results in an increased rate of single-base changes in the newly synthesized DNA strand. Which function of which protein is MOST likely disrupted in this situation?

You run a PCR reaction for five cycles starting with a single DNA duplex. Theoretically, how many copies of your sequence would you now have?

Which of the following make(s) the Southern blot technique possible?

A DNA molecule is cut with two different restriction enzymes known to cleave it only once each. After gel electrophoresis, three different DNA fragments are detected. This result means that the original DNA molecule was:

Life: Chemical, Cellular, and Evolutionary Foundations

The Molecules of Life

Nucleic Acids and Transcription

Translation and Protein Structure

Organizing Principles: Lipids, Membranes, and Cell Compartments

Making Life Work: Capturing and Using Energy

Cellular Respiration: Harvesting Energy From Carbohydrates and Other Fuel Molecules

Photosynthesis: Using Sunlight to Build Carbohydrates

Cell Signaling

Cell and Tissue Architecture: Cytoskeleton, Cell Junctions, and Extracellular Matrix

Cell Division: Variations, Regulation, and Cancer

Genomes

Mutation and Dna Repair

Genetic Variation

Mendelian Inheritance

Inheritance of Sex Chromosomes, Linked Genes, and Organelles

The Genetic and Environmental Basis of Complex Traits

Genetic and Epigenetic Regulation

Genes and Development

Evolution: How Genotypes and Phenotypes Change Over Time

Species and Speciation

Evolutionary Patterns: Phylogeny and Fossils

Human Origins and Evolution

Cycling Carbon

Bacteria and Archaea

Eukaryotic Cells: Origins and Diversity

Being Multicellular

Plant Structure and Function: Moving Photosynthesis Onto Land

Plant Reproduction: Finding Mates and Dispersing Young

Plant Growth and Development

Plant Defense: Keeping the World Green

Plant Diversity

Fungi: Structure, Function, and Diversity

Animal Nervous Systems

Animal Sensory Systems and Brain Function

Animal Movement: Muscles and Skeletons

Animal Endocrine Systems

Animal Cardiovascular and Respiratory Systems

Animal Metabolism, Nutrition, and Digestion

Animal Renal Systems: Water and Waste

Animal Reproduction and Development

Animal Immune Systems

Animal Diversity

Animal Behavior

Population Ecology

Species Interactions, Communities, and Ecosystems

Biomes and Global Ecology

The Anthropocene: Humans As a Planetary Force

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