Exam 12: Dna Replication and Manipulation

If a restriction enzyme has a cleavage site consisting of six nucleotide pairs, what is the average distance in nucleotides between cleavage sites in a random sequence of double-stranded DNA?

RNA is involved in which of the following cellular processes?

Which of the following is NOT needed for DNA replication?

Telomerase is fully active in _____ and _____ cells, but almost completely inactive in _____ cells.

Why are single-stranded binding proteins necessary for DNA replication?

Which of the following make(s) sequencing by the Sanger chain-termination method possible?

Using PCR, a student hopes to amplify a sequence of known DNA from a genomic DNA sample. The two primers are both designed to pair with sequences that have a 40% GC content. Using a particular set of denaturation temperatures, annealing temperatures, and extension temperatures, the experiment produces not the expected single DNA fragment of a single known size, but a set of fragments of different sizes. Which of the following could account for these results?

In DNA editing by means of CRISPR, the function of the CRISPR-associated protein is to:

One application of GMOs is to engineer mice that have particular diseases that scientists wish to study.

Organisms that have been modified to contain DNA from other species are known as:

The transformation step in creating bacteria genetically engineered to produce human proteins involves:

Stem cells located in the gastrointestinal tract would possess more inactive telomerase compared to the amount of telomerase activity in mature neurons of the brain.

The enzyme _____ compensates for chromosomes shortening at the tips after each round of DNA replication.

A certain restriction enzyme cleaves double-stranded DNA at the sequence shown below, where the slash indicates where each strand is cleaved. Note that the cleavage results in a two-base pair single-stranded region at the 5' end that allows the cleaved ends to undergo base pairing. 5'-AA/ATTT-3' 3'-TTTA/AA-5' Which of the restriction enzymes, that cleave double-stranded DNA as shown below, would produce overhanging ends able to pair with those produced by the enzyme described above?

Recombinant DNA only occurs when you take DNA from another organism and insert it into a bacterium.

Okazaki fragments are found on _____ strand of DNA.

To follow up on your previous experiment with DNA replication in virus U238, conduct another Meselson-Stahl type experiment, again allowing a virus to replicate in the presence of "heavy" nitrogen until both strands of its genome are "heavy." In this experiment, you allow virus U238 to undergo two complete rounds of replication in the presence of "light" nitrogen. What ratio of "heavy" to "light" viral DNA would you expect to find at the end of two complete rounds of replication?

If a restriction site of AatII is 5'-GACGTC -3' then 3'-GACGTC-5' is also an AatII restriction site.

A Southern blot is a technique that relies on hybridization of:

A lack of telomerase activity limits what?