Exam 18: Gene Expression: Ithe Genetic Code and Transcription

According to the central dogma,the process by which information in an RNA molecule is used to direct the synthesis of a polypeptide is called

The action of a new drug is unknown,so you test various aspects of eukaryotic cell processes looking for an effect.You notice that the survival of cells exposed to this drug is very low.Further analysis shows that some proteins and mRNAs are much longer than they should be.One hypothesis is that the drug interferes with

With respect to RNA processing,which of the following is false?

Answer the questions below based on this RNA sequences: 5'-AUGAUGAUGAUG-3' This RNA transcript was generated using a synthetic DNA template. The letters B, D, E, F, G, and H have been selected to represent different amino acids. Assume that each codon is a different amino acid -Imagine that the genetic code were read in an overlapping fashion,using three nucleotides to specify each amino acid (i.e.,the sequence above would be read: AUG-UGA-GUA and so on).Which of the following polypeptides would be obtained by translating the sequence above?

With regard to RNA polymerase proofreading ability,which of the following is true?

You have identified a protein that you hypothesize is a sigma factor in E.coli that has not yet been characterized.You have purified the protein.What types of experiments could you perform to investigate whether or not the protein is a sigma factor?

________-driven promoters are comprised of an Inr sequence and a TATA box with or without an associated BRE.

You have isolated a piece of DNA that you believe contains an interesting gene.Using a mix of RNA polymerase and ribonucleotides,you perform in vitro transcription.However,even though all of your controls work,no mRNA is created from your DNA fragment.Working backward,you note that your DNA preparation removed approximately 250 base pairs from the 5' end of the gene.The most likely explanation is that during DNA preparation

An example of retrotransposons in the human genome is the ________ family of sequences.

If you were designing a method to specifically inhibit prokaryotic transcription,but not eukaryotic transcription,interfering with which of the following would work best?

You wish to design a drug that acts against a recently identified retrovirus.Based upon what you know about retroviruses,which of the following would be the best target for specifically interrupting the viral life cycle in eukaryotic cells?

Which of the following is not a reason for the importance of mRNA processing in eukaryotes?

Which of the following DNA regions is recognized by RNA polymerase during transcription?

Which of the following patterns of information flow is unique to retroviruses within a cell?