Question 1

The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is

Accepted Answer

A)  isolate the desired gene - purify the expressed protein - grow the transformed cells into suitable quantities - insert the gene into an expression vector - transform host cells with the expression vector. 
B)  purify the expressed protein - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene. 
C)  grow the transformed cells into suitable quantities - transform host cells with the expression vector - insert the gene into an expression vector -isolate the desired gene - purify the expressed protein. 
D)  transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene - insert the gene into an expression vector - purify the expressed protein. 
E)  isolate the desired gene - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - purify the expressed protein. 
A)  isolate the desired gene - purify the expressed protein - grow the transformed cells into suitable quantities - insert the gene into an expression vector - transform host cells with the expression vector. 
B)  purify the expressed protein - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene. 
C)  grow the transformed cells into suitable quantities - transform host cells with the expression vector - insert the gene into an expression vector -isolate the desired gene - purify the expressed protein. 
D)  transform host cells with the expression vector - grow the transformed cells into suitable quantities - isolate the desired gene - insert the gene into an expression vector - purify the expressed protein. 
E)  isolate the desired gene - insert the gene into an expression vector - transform host cells with the expression vector - grow the transformed cells into suitable quantities - purify the expressed protein.

Question 2

The interferon- gamma release assay for tuberculosis infection works because

Accepted Answer

A)  the TB bacterium releases interferon- gamma. 
B)  the presence of interferon- gamma indicates a person is susceptible to TB infection. 
C)  the T cells of an infected person release more interferon- gamma than that of a non- infected person. 
D)  individuals who have had the TB vaccine can be identified by the amount of interferon- gamma in their blood. 
E)  one can perform an indirect ELISA to detect antibodies to interferon- gamma in a patient with TB. 
A)  the TB bacterium releases interferon- gamma. 
B)  the presence of interferon- gamma indicates a person is susceptible to TB infection. 
C)  the T cells of an infected person release more interferon- gamma than that of a non- infected person. 
D)  individuals who have had the TB vaccine can be identified by the amount of interferon- gamma in their blood. 
E)  one can perform an indirect ELISA to detect antibodies to interferon- gamma in a patient with TB.

Question 3

In a recombinant vector vaccine, genetic material from a pathogen is placed into a and introduced to human cells.

Accepted Answer

A)  harmless virus or bacteria 
B)  plasmid vector 
C)  human B or T cell 
D)  pathogenic virus or bacteria 
E)  human dendritic cell 
A)  harmless virus or bacteria 
B)  plasmid vector 
C)  human B or T cell 
D)  pathogenic virus or bacteria 
E)  human dendritic cell

Question 4

A drawback of all inactivated vaccines is that

Accepted Answer

A)  they are likely to cause disease in immunocompromised hosts. 
B)  they may mutate to a more virulent form. 
C)  they require complex genetic engineering techniques to produce. 
D)  they may cause secondary transmission from the immunized person to another host. 
E)  they are quickly cleared from the body, limiting antigen exposure. 
A)  they are likely to cause disease in immunocompromised hosts. 
B)  they may mutate to a more virulent form. 
C)  they require complex genetic engineering techniques to produce. 
D)  they may cause secondary transmission from the immunized person to another host. 
E)  they are quickly cleared from the body, limiting antigen exposure.

Question 5

DNA microarrays reveal

Accepted Answer

A)  if reverse transcriptase is present in a given cell. 
B)  which genes are found in a given cell. 
C)  which genes are being expressed in a given cell. 
D)  if a particular gene was acquired via horizontal gene transfer. 
E)  if any genes are mutated in a given cell. 
A)  if reverse transcriptase is present in a given cell. 
B)  which genes are found in a given cell. 
C)  which genes are being expressed in a given cell. 
D)  if a particular gene was acquired via horizontal gene transfer. 
E)  if any genes are mutated in a given cell.

Question 6

A vaccine additive which enhances the body's natural immune response is called a(n)

Accepted Answer

A)  recombinant. 
B)  attenuant. 
C)  adjuvant. 
D)  booster. 
E)  toxoid. 
A)  recombinant. 
B)  attenuant. 
C)  adjuvant. 
D)  booster. 
E)  toxoid.

Question 7

In the figure, gray figures represent non- immune individuals; black figures represent immune individuals. Which population will be least likely to experience disease outbreak?

Accepted Answer

A)  Population 1 
B)  Population 2 
C)  Population 3 
D)  None of the populations 
E)  All of the populations 
A)  Population 1 
B)  Population 2 
C)  Population 3 
D)  None of the populations 
E)  All of the populations

The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is

The interferon- gamma release assay for tuberculosis infection works because

In a recombinant vector vaccine, genetic material from a pathogen is placed into a and introduced to human cells.

A drawback of all inactivated vaccines is that

DNA microarrays reveal

A vaccine additive which enhances the body's natural immune response is called a(n)

In the figure, gray figures represent non- immune individuals; black figures represent immune individuals. Which population will be least likely to experience disease outbreak?

Introduction to Microbiology

Biochemistry Basics

Introduction to Prokaryotic Cells

Introduction to Eukaryotic Cells

Genetics

Viruses and Prions

Fundamentals of Microbial Growth

Microbial Metabolism

Principles of Infectious Disease and Epidemiology

Host-Microbe Interactions and Pathogenesis

Innate Immunity

Adaptive Immunity

Immune System Disorders

Antimicrobial Drugs

Respiratory System Infections

Skin and Eye Infections

Nervous System Infections

Digestive System Infections

Urinary and Reproductive System Infections

Cardiovascular and Lymphatic Infections

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Exam 14: Vaccines and Biotechnology-Based Diagnostics and Therapeutics

The correct order of steps to follow when using recombinant DNA technology to produce a protein for pharmacological use is

The interferon- gamma release assay for tuberculosis infection works because

In a recombinant vector vaccine, genetic material from a pathogen is placed into a and introduced to human cells.

A drawback of all inactivated vaccines is that

DNA microarrays reveal

A vaccine additive which enhances the body's natural immune response is called a(n)

In the figure, gray figures represent non- immune individuals; black figures represent immune individuals. Which population will be least likely to experience disease outbreak?

Introduction to Microbiology

Biochemistry Basics

Introduction to Prokaryotic Cells

Introduction to Eukaryotic Cells

Genetics

Viruses and Prions

Fundamentals of Microbial Growth

Microbial Metabolism

Principles of Infectious Disease and Epidemiology

Host-Microbe Interactions and Pathogenesis

Innate Immunity

Adaptive Immunity

Immune System Disorders

Antimicrobial Drugs

Respiratory System Infections

Skin and Eye Infections

Nervous System Infections

Digestive System Infections

Urinary and Reproductive System Infections

Cardiovascular and Lymphatic Infections

Filters