Question 1

Some genetically modified tobacco plants can express the light-producing chemicals present in fireflies.

Accepted Answer

A) True 
 B)False  True

Question 2

Match each term with its definition.
-DNA hybridization

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome F

Question 3

Match each term with the most appropriate macromolecule being studied or manipulated.
-cloning

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein A

Question 4

What do restriction endonucleases do?
a.break glycosidic bonds
b.break ester bonds
c.break phosphodiester bonds
d.break peptide bonds

Accepted Answer

The answer of What do restriction endonucleases do?
a.break glycosidic bonds
b.break...

Question 5

Different methodologies can often be used to obtain the same information about a DNA sequence.

Accepted Answer

A) True 
 B)False

Question 6

-According to the table, which enzymes were used to digest a DNA fragment that results in the following sequence? GATCC---------AT
G---------TAGC

Accepted Answer

A) BamHI and ClaI 
B) HindIII and BamHI 
C) EcoRI and HindIII 
D) ClaI and PvuI 
A) BamHI and ClaI 
B) HindIII and BamHI 
C) EcoRI and HindIII 
D) ClaI and PvuI

Question 7

Match each term with its definition.
-transgenic

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 8

Match each term with its definition.
-structural genomics

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 9

In which stage is the foreign gene introduced into the developing embryo during the creation of transgenic animals?

Accepted Answer

A) at the blastocyst stage, via a transgenic cell 
B) in cell culture, prior to fertilization 
C) at the sperm stage, via direct microinjection of the new DNA 
D) at the egg stage, via direct microinjection of the new DNA 
A) at the blastocyst stage, via a transgenic cell 
B) in cell culture, prior to fertilization 
C) at the sperm stage, via direct microinjection of the new DNA 
D) at the egg stage, via direct microinjection of the new DNA

Question 10

Match each term with the most appropriate macromolecule being studied or manipulated.
-proteomics

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 11

Some restriction endonucleases cut the DNA in such a way that short, single-stranded regions are created. What are these regions called?

Accepted Answer

A) blunt ends 
B) tacky ends 
C) hydrogen-bonding ends 
D) sticky ends 
A) blunt ends 
B) tacky ends 
C) hydrogen-bonding ends 
D) sticky ends

Question 12

Match each term with its definition.
-genetic engineering

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 13

Match the names of the processes with the descriptions.
-This is the methodology that would be considered by a scientist who wants to generate mice that express a fluorescent protein in every cell of their body.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 14

Match each term with its definition.
-restriction endonucleases

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 15

Genetically modified crops have been banned in Canada.

Accepted Answer

A) True 
 B)False

Question 16

Where is cloned DNA in bacteria usually found?

Accepted Answer

A) on a virus 
B) on the flagellum 
C) on a plasmid 
D) on the bacterial chromosome 
A) on a virus 
B) on the flagellum 
C) on a plasmid 
D) on the bacterial chromosome

Question 17

Match each term with its definition.
-restriction fragments

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 18

Which of the following is needed in order to amplify the DNA for gene X and NOT for gene Y?

Accepted Answer

A) a heat-stable DNA polymerase 
B) restriction endonucleases that cut gene X and not gene Y 
C) plasmid vectors that have multiple antibiotic resistance genes 
D) some prior knowledge of the sequence of gene X such that appropriate primers can be designed 
A) a heat-stable DNA polymerase 
B) restriction endonucleases that cut gene X and not gene Y 
C) plasmid vectors that have multiple antibiotic resistance genes 
D) some prior knowledge of the sequence of gene X such that appropriate primers can be designed

Question 19

Match each term with the most appropriate macromolecule being studied or manipulated.
-microarray

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 20

Match each term with the most appropriate macromolecule being studied or manipulated.
-PCR

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Exam 15: Dna Technologies

Some genetically modified tobacco plants can express the light-producing chemicals present in fireflies.

Match each term with its definition. -DNA hybridization

Match each term with the most appropriate macromolecule being studied or manipulated. -cloning

What do restriction endonucleases do? a.break glycosidic bonds b.break ester bonds c.break phosphodiester bonds d.break peptide bonds

Different methodologies can often be used to obtain the same information about a DNA sequence.

-According to the table, which enzymes were used to digest a DNA fragment that results in the following sequence? GATCC---------AT G---------TAGC

Match each term with its definition. -transgenic

Match each term with its definition. -structural genomics

In which stage is the foreign gene introduced into the developing embryo during the creation of transgenic animals?

Match each term with the most appropriate macromolecule being studied or manipulated. -proteomics

Some restriction endonucleases cut the DNA in such a way that short, single-stranded regions are created. What are these regions called?

Match each term with its definition. -genetic engineering

Match the names of the processes with the descriptions. -This is the methodology that would be considered by a scientist who wants to generate mice that express a fluorescent protein in every cell of their body.

Match each term with its definition. -restriction endonucleases

Genetically modified crops have been banned in Canada.

Where is cloned DNA in bacteria usually found?

Match each term with its definition. -restriction fragments

Which of the following is needed in order to amplify the DNA for gene X and NOT for gene Y?

Match each term with the most appropriate macromolecule being studied or manipulated. -microarray

Match each term with the most appropriate macromolecule being studied or manipulated. -PCR

Light and Life

The Cell: an Overview

Defining Life and Its Origins

Energy and Enzymes

Cell Membranes and Signalling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

Mendel, Genes, and Inheritance

Genes, Chromosomes, and Human Genetics

Dna Structure, Replication, and Organization

Gene Structure and Expression

Control of Gene Expression

Genomes and Proteomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Species and Macroevolution

Understanding the History of Life on Earth

Humans and Evolution

Bacteria and Archaea

Viruses, Viroids, and Prions: Infectious Biological Particles

Protists

Fungi

Plants

Diversity of Animals 1: Sponges, Radiata, Platyhelminthes, and Protostomes

Diversity of Animals 2: Deuterostomes: Vertebrates and Their Closest Relatives

Population Ecology

Population Interactions and Community Ecology

Ecosystems

Conservation of Biodiversity

Putting Selection to Work

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Transport in Animals: the Circulatory System

Reproduction in Animals

Animal Development

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Integration

Muscles, Skeletons, and Body Movements

Animal Behaviour

Animal Nutrition

Gas Exchange: the Respiratory System

Regulating the Internal Environment

Defences Against Disease

Conservation and Evolutionary Physiology

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