Question 1

Match each term with its definition.
-DNA fingerprinting

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 2

How is the Ti plasmid used to create transgenic plants?

Accepted Answer

A) The Ti plasmid inserts the foreign gene directly into the plant's nuclear DNA. 
B) Once the Rhizobium radiobacter forms a tumour in the plant, the tumour cells can take up the foreign DNA and incorporate it into their chromosomes. 
C) The Ti plasmid allows all of the plant cells to host the bacteria Rhizobium radiobacter and thus express the foreign gene that was inserted into the bacterial chromosome. 
D) The Ti plasmid inserts the foreign DNA into the DNA in the chloroplast. 
A) The Ti plasmid inserts the foreign gene directly into the plant's nuclear DNA. 
B) Once the Rhizobium radiobacter forms a tumour in the plant, the tumour cells can take up the foreign DNA and incorporate it into their chromosomes. 
C) The Ti plasmid allows all of the plant cells to host the bacteria Rhizobium radiobacter and thus express the foreign gene that was inserted into the bacterial chromosome. 
D) The Ti plasmid inserts the foreign DNA into the DNA in the chloroplast.

Question 3

Which of the following describes biotechnology?

Accepted Answer

A) any technique applied to dead organisms to make or modify products or processes for a specific purpose. 
B) any technique applied to non-biological systems or dead organisms to make or modify products or processes for a specific purpose 
C) any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose 
D) any technique applied to non-biological systems to make or modify products or processes for a specific purpose 
A) any technique applied to dead organisms to make or modify products or processes for a specific purpose. 
B) any technique applied to non-biological systems or dead organisms to make or modify products or processes for a specific purpose 
C) any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose 
D) any technique applied to non-biological systems to make or modify products or processes for a specific purpose

Question 4

What are the three essential pieces of DNA required for PCR?

Accepted Answer

A) one primer and a template 
B) two primers and a template 
C) one primer and unknown DNA 
D) two primers and unknown DNA 
A) one primer and a template 
B) two primers and a template 
C) one primer and unknown DNA 
D) two primers and unknown DNA

Question 5

Match each term with the most appropriate macromolecule being studied or manipulated.
-genomics

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 6

Which of the following distinguishes DNA cloning from gene cloning?

Accepted Answer

A) DNA cloning is a method for producing a few copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
B) DNA cloning is a method for producing a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
C) Gene cloning is a method for producing many copies of a piece of DNA. DNA cloning is cloning that involves a gene. 
D) DNA cloning is a method for producing many copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
A) DNA cloning is a method for producing a few copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
B) DNA cloning is a method for producing a piece of DNA. Gene cloning is DNA cloning that involves a gene. 
C) Gene cloning is a method for producing many copies of a piece of DNA. DNA cloning is cloning that involves a gene. 
D) DNA cloning is a method for producing many copies of a piece of DNA. Gene cloning is DNA cloning that involves a gene.

Question 7

Match each term with the most appropriate macromolecule being studied or manipulated.
-fingerprinting

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 8

Match each term with the most appropriate macromolecule being studied or manipulated.
-blotting

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 9

What are restriction fragment length polymorphisms used for?

Accepted Answer

A) to prepare DNA for further sequence analysis 
B) to treat sickle cell anemia 
C) to test the effectiveness of different restriction enzymes on a sequence of DNA 
D) to compare DNA from two individuals 
A) to prepare DNA for further sequence analysis 
B) to treat sickle cell anemia 
C) to test the effectiveness of different restriction enzymes on a sequence of DNA 
D) to compare DNA from two individuals

Question 10

Why would researchers want to have both genomic libraries and cDNA libraries from a particular organism?

Accepted Answer

A) because a cDNA library helps us understand how retroviruses work, but a genomic library does not 
B) because a genomic library is generally too big to be helpful, while a cDNA library, being smaller, is much easier to study 
C) because a genomic library will be the same in every cell, while a cDNA library tells researchers which genes are being expressed in individual cell types 
D) because a cDNA library is a lot easier to clone than a genomic library 
A) because a cDNA library helps us understand how retroviruses work, but a genomic library does not 
B) because a genomic library is generally too big to be helpful, while a cDNA library, being smaller, is much easier to study 
C) because a genomic library will be the same in every cell, while a cDNA library tells researchers which genes are being expressed in individual cell types 
D) because a cDNA library is a lot easier to clone than a genomic library

Question 11

How are the sticky ends obtained?

Accepted Answer

A) by using a different restriction enzyme to cut both DNA fragment and the plasmid 
B) by using the same restriction enzyme to cut both DNA fragment and the plasmid 
C) by using a restriction enzyme to cut DNA fragment 
D) by using a restriction enzyme to cut the plasmid 
A) by using a different restriction enzyme to cut both DNA fragment and the plasmid 
B) by using the same restriction enzyme to cut both DNA fragment and the plasmid 
C) by using a restriction enzyme to cut DNA fragment 
D) by using a restriction enzyme to cut the plasmid

Question 12

Sticky ends are ends on DNA fragments that are complementary to the DNA ends on the open plasmid.

Accepted Answer

A) True 
 B)False

Question 13

to identify samples with specific DNA sequences
-Which of the following can be determined by DNA fingerprinting?

Accepted Answer

A) the sequence of human genome 
B) the genome of human fingers 
C) the difference between two individuals of the same species 
D) the species which a sample came from 
A) the sequence of human genome 
B) the genome of human fingers 
C) the difference between two individuals of the same species 
D) the species which a sample came from

Question 14

Match the names of the processes with the descriptions.
-This methodology lets you study the complete set of protein that can be expressed by an organism's genome.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 15

When using embryonic germ-line cells to create transgenic mice, the offspring of the genetically modified animal will have all transgenic cells in their bodies.

Accepted Answer

A) True 
 B)False

Question 16

Match each term with its definition.
-functional genomics

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 17

What is the natural function of the T DNA contained within the Ti plasmid?

Accepted Answer

A) to increase diversity 
B) to induce tumours 
C) to allow for recombination 
D) to transfer genes 
A) to increase diversity 
B) to induce tumours 
C) to allow for recombination 
D) to transfer genes

Question 18

Suppose that multiple fragments from a genome have been cloned into bacteria. How can researchers identify the bacteria that contain the particular sequence they are interested in?

Accepted Answer

A) by sequencing all the bacterial DNA 
B) by DNA hybridization, using a labelled probe 
C) by observing that the bacteria will produce white colonies on X-gal 
D) by isolating the DNA of interest before transformation 
A) by sequencing all the bacterial DNA 
B) by DNA hybridization, using a labelled probe 
C) by observing that the bacteria will produce white colonies on X-gal 
D) by isolating the DNA of interest before transformation

Question 19

Match each term with its definition.
-cDNA library

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 20

Which approach would be most helpful in determining whether the gene for a particular protein is the same length in frogs, humans, and trees?

Accepted Answer

A) use cDNA library construction and agarose gel electrophoresis 
B) use genomic library construction and PCR 
C) use DNA cloning and DNA hybridization 
D) use PCR and agarose gel electrophoresis 
A) use cDNA library construction and agarose gel electrophoresis 
B) use genomic library construction and PCR 
C) use DNA cloning and DNA hybridization 
D) use PCR and agarose gel electrophoresis

Exam 15: Dna Technologies

Match each term with its definition. -DNA fingerprinting

How is the Ti plasmid used to create transgenic plants?

Which of the following describes biotechnology?

What are the three essential pieces of DNA required for PCR?

Match each term with the most appropriate macromolecule being studied or manipulated. -genomics

Which of the following distinguishes DNA cloning from gene cloning?

Match each term with the most appropriate macromolecule being studied or manipulated. -fingerprinting

Match each term with the most appropriate macromolecule being studied or manipulated. -blotting

What are restriction fragment length polymorphisms used for?

Why would researchers want to have both genomic libraries and cDNA libraries from a particular organism?

How are the sticky ends obtained?

Sticky ends are ends on DNA fragments that are complementary to the DNA ends on the open plasmid.

to identify samples with specific DNA sequences -Which of the following can be determined by DNA fingerprinting?

Match the names of the processes with the descriptions. -This methodology lets you study the complete set of protein that can be expressed by an organism's genome.

When using embryonic germ-line cells to create transgenic mice, the offspring of the genetically modified animal will have all transgenic cells in their bodies.

Match each term with its definition. -functional genomics

What is the natural function of the T DNA contained within the Ti plasmid?

Suppose that multiple fragments from a genome have been cloned into bacteria. How can researchers identify the bacteria that contain the particular sequence they are interested in?

Match each term with its definition. -cDNA library

Which approach would be most helpful in determining whether the gene for a particular protein is the same length in frogs, humans, and trees?

Light and Life

The Cell: an Overview

Defining Life and Its Origins

Energy and Enzymes

Cell Membranes and Signalling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

Mendel, Genes, and Inheritance

Genes, Chromosomes, and Human Genetics

Dna Structure, Replication, and Organization

Gene Structure and Expression

Control of Gene Expression

Genomes and Proteomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Species and Macroevolution

Understanding the History of Life on Earth

Humans and Evolution

Bacteria and Archaea

Viruses, Viroids, and Prions: Infectious Biological Particles

Protists

Fungi

Plants

Diversity of Animals 1: Sponges, Radiata, Platyhelminthes, and Protostomes

Diversity of Animals 2: Deuterostomes: Vertebrates and Their Closest Relatives

Population Ecology

Population Interactions and Community Ecology

Ecosystems

Conservation of Biodiversity

Putting Selection to Work

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Transport in Animals: the Circulatory System

Reproduction in Animals

Animal Development

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Integration

Muscles, Skeletons, and Body Movements

Animal Behaviour

Animal Nutrition

Gas Exchange: the Respiratory System

Regulating the Internal Environment

Defences Against Disease

Conservation and Evolutionary Physiology

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