Question 1

Gene therapy has been successful in curing some individuals of autoimmune disorder and sickle cell disease.

Accepted Answer

A) True 
 B)False

Question 2

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You would use this tool if you wanted to check if a particular gene was contained in an organism's genome.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 3

Which of the following refer to nonrecombinant plasmids?

Accepted Answer

A) resealed cloning vectors with no DNA fragment inserted 
B) plasmids with DNA fragments inserted into the cloning vector 
C) plaids with no DNA fragment inserted 
D) resealed cloning vectors with DNA fragments inserted into the cloning vector 
A) resealed cloning vectors with no DNA fragment inserted 
B) plasmids with DNA fragments inserted into the cloning vector 
C) plaids with no DNA fragment inserted 
D) resealed cloning vectors with DNA fragments inserted into the cloning vector

Question 4

DNA fingerprinting is limited to human studies.

Accepted Answer

A) True 
 B)False

Question 5

-According to the table, which enzymes should be used to generate a double-stranded sequence that looks like the one below? AGCTT---------G
A---------CCTAG

Accepted Answer

A) ClaI and PvuI 
B) BamHI and ClaI 
C) EcoRI and HindIII 
D) HindIII and BamHI 
A) ClaI and PvuI 
B) BamHI and ClaI 
C) EcoRI and HindIII 
D) HindIII and BamHI

Question 6

Which of the following describes DNA ligase?

Accepted Answer

A) the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce an original DNA molecule 
B) the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
C) the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
D) the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant RNA molecule 
A) the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce an original DNA molecule 
B) the enzyme that seals together DNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
C) the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant DNA molecule 
D) the enzyme that seals together RNA fragments generated by restriction enzyme digestion to produce a recombinant RNA molecule

Question 7

Define the purpose of cloning a gene into a plasmid cloning vector.

Accepted Answer

By cloning a gene into a vector, you can

Question 8

Match the names of the processes with the descriptions.
-This is any technique applied to living organisms that modifies or makes them for a specific purpose.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 9

What has genetic engineering been used for?

Accepted Answer

A) to create mice with altered genomes so we can model certain human diseases 
B) to produce a vaccine against hoof-and-mouth disease 
C) to create plants that are more resistant to certain pests or herbicides 
D) to induce bacteria to mass-produce human insulin 
A) to create mice with altered genomes so we can model certain human diseases 
B) to produce a vaccine against hoof-and-mouth disease 
C) to create plants that are more resistant to certain pests or herbicides 
D) to induce bacteria to mass-produce human insulin

Question 10

In what way are basic and applied research studies of a cloned gene different?

Accepted Answer

A) In basic research, a researcher wants to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for private application. 
B) In basic research, a researcher does not want to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for medical application. 
C) In basic research, a researcher does not learn about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is in the structure and function of a gene. 
D) In basic research, a researcher learns about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is not in the structure and function of a gene. 
A) In basic research, a researcher wants to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for private application. 
B) In basic research, a researcher does not want to study the gene's function, including how its expression is regulated, and the nature of the gene's product, while in applied research, the interest is in using cloned genes for medical application. 
C) In basic research, a researcher does not learn about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is in the structure and function of a gene. 
D) In basic research, a researcher learns about the gene's structure, including its DNA sequence and sequences that regulate its expression, while in applied research, the interest is not in the structure and function of a gene.

Question 11

If you were to utilize "gene machine," which of the following would you be using?

Accepted Answer

A) a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input 
B) a machine that can create DNA sequences of choice by manual chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
C) a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
D) a machine that can create RNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input 
A) a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input 
B) a machine that can create DNA sequences of choice by manual chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
C) a machine that can create DNA sequences of choice by automated chemical synthesis in the laboratory using a DNA synthesizer responding to computer input 
D) a machine that can create RNA sequences of choice by automated chemical synthesis in the laboratory using a RNA synthesizer responding to computer input

Exam 15: Dna Technologies

Gene therapy has been successful in curing some individuals of autoimmune disorder and sickle cell disease.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You would use this tool if you wanted to check if a particular gene was contained in an organism's genome.

Which of the following refer to nonrecombinant plasmids?

DNA fingerprinting is limited to human studies.

-According to the table, which enzymes should be used to generate a double-stranded sequence that looks like the one below? AGCTT---------G A---------CCTAG

Which of the following describes DNA ligase?

Define the purpose of cloning a gene into a plasmid cloning vector.

Match the names of the processes with the descriptions. -This is any technique applied to living organisms that modifies or makes them for a specific purpose.

What has genetic engineering been used for?

In what way are basic and applied research studies of a cloned gene different?

If you were to utilize "gene machine," which of the following would you be using?

Light and Life

The Cell: an Overview

Defining Life and Its Origins

Energy and Enzymes

Cell Membranes and Signalling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

Mendel, Genes, and Inheritance

Genes, Chromosomes, and Human Genetics

Dna Structure, Replication, and Organization

Gene Structure and Expression

Control of Gene Expression

Genomes and Proteomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Species and Macroevolution

Understanding the History of Life on Earth

Humans and Evolution

Bacteria and Archaea

Viruses, Viroids, and Prions: Infectious Biological Particles

Protists

Fungi

Plants

Diversity of Animals 1: Sponges, Radiata, Platyhelminthes, and Protostomes

Diversity of Animals 2: Deuterostomes: Vertebrates and Their Closest Relatives

Population Ecology

Population Interactions and Community Ecology

Ecosystems

Conservation of Biodiversity

Putting Selection to Work

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Transport in Animals: the Circulatory System

Reproduction in Animals

Animal Development

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Integration

Muscles, Skeletons, and Body Movements

Animal Behaviour

Animal Nutrition

Gas Exchange: the Respiratory System

Regulating the Internal Environment

Defences Against Disease

Conservation and Evolutionary Physiology

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