Question 1

Match each term with the most appropriate macromolecule being studied or manipulated.
-transgenics

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 2

Which of the following is characteristic of cDNA?

Accepted Answer

A) a double stranded DNA copy of a single-stranded mRNA molecule 
B) a single stranded DNA copy of a single-stranded mRNA molecule 
C) a single stranded mRNA copy of a double-stranded mRNA molecule 
D) a single stranded mRNA copy of a single-stranded mRNA molecule 
A) a double stranded DNA copy of a single-stranded mRNA molecule 
B) a single stranded DNA copy of a single-stranded mRNA molecule 
C) a single stranded mRNA copy of a double-stranded mRNA molecule 
D) a single stranded mRNA copy of a single-stranded mRNA molecule

Question 3

Match the names of the processes with the descriptions.
-You use this technique to produce recombinant DNA.

Accepted Answer

A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics 
A) DNA fingerprinting 
B) transgenics 
C) DNA cloning 
D) biotechnology 
E) proteomics

Question 4

Match each term with the most appropriate macromolecule being studied or manipulated.
-sequencing

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 5

What drawback have researchers noted in the cloning of domestic animals?

Accepted Answer

A) The cloned animals often suffer from birth defects. 
B) The cloned animals do not resemble the parent. 
C) The cloned animals do not reach adulthood. 
D) The cloned animals are not viable. 
A) The cloned animals often suffer from birth defects. 
B) The cloned animals do not resemble the parent. 
C) The cloned animals do not reach adulthood. 
D) The cloned animals are not viable.

Question 6

Gene therapy is a safe, effective, and commercially available treatment for numerous genetic disorders.

Accepted Answer

A) True 
 B)False

Question 7

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-This technique will let you move a gene into a vector for expression in different cells.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 8

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-You would use this to cut DNA at a selected site.

Accepted Answer

A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR 
A) DNA library 
B) restriction enzyme 
C) gene cloning 
D) Southern blotting 
E) PCR

Question 9

Match each term with the most appropriate macromolecule being studied or manipulated.
-agarose gel electrophoresis

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 10

The list below contains steps for cloning a gene of interest. They are in random order, and some may be incorrect. Which sequence places the correct stems in the correct order?
1)Transform the DNA into E. coli.
2)Ligate cut genomic DNA fragments and cut plasmid DNA together using DNA ligase.
3)Digest the plasmid cloning vector with the same restriction enzyme.
4)Isolate genomic DNA and digest that DNA with a restriction enzyme.
5)Spread bacterial cells on medium containing lactose and ampicillin and incubate to allow colonies to grow.
6)Spread the bacterial cells on growth medium containing ampicillin and X-gal and incubate to allow colonies to grow.

Accepted Answer

A) 4, 3, 2, 1, 5 
B) 4, 2, 3, 1, 5 
C) 4, 2, 3, 1, 6 
D) 4, 3, 2, 1, 6 
A) 4, 3, 2, 1, 5 
B) 4, 2, 3, 1, 5 
C) 4, 2, 3, 1, 6 
D) 4, 3, 2, 1, 6

Question 11

Match each term with the most appropriate macromolecule being studied or manipulated.
-cDNA library

Accepted Answer

A) DNA 
B) mRNA/cDNA 
C) protein 
A) DNA 
B) mRNA/cDNA 
C) protein

Question 12

Explain the ethical concerns with germ-line gene therapy in humans.

Accepted Answer

If gene therapy were applied to germ-lin

Question 13

Match each term with its definition.
-agarose gel electrophoresis

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 14

Match each term with its definition.
-systems biology

Accepted Answer

A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome 
A) uses DNA technologies to alter genes in a cell or organism 
B) branch of biology that tries to combine proteomics and genomics at an organismal level 
C) determination and analysis of the nucleotide sequences in the chromosomes in an organism 
D) products of a restriction endonuclease's action 
E) collection of clones that together contain every DNA sequence in a genome, including noncoding sequences
F)use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify it
G)collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell type
H)procedure described as "a photocopy machine for specific DNA sequences"
I)DNA from two different sources that have been joined together into a single molecule
J)term used to describe organisms that have been subject to genetic engineering
K)study of all of the proteins produced from an organism's genome
L)study of the functions of genes and noncoding portions of the genome, including their interactions with each other
M)bacterial enzymes that recognize and cut specific DNA sequences
N)method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresis
O)technique used to separate DNA molecules based on their relative sizes
P)method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genome

Question 15

Why is there X-gal used in the cloning medium?

Accepted Answer

A) It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by black-white screening. 
B) It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by blue-white screening. 
C) It distinguishes between plasmids that have been transformed and those that have not by blue-white screening. 
D) It distinguishes between plasmids that have been transformed and those that have not by black-white screening. 
A) It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by black-white screening. 
B) It distinguishes between bacteria that have been transformed with recombinant plasmids and nonrecombinant plasmids by blue-white screening. 
C) It distinguishes between plasmids that have been transformed and those that have not by blue-white screening. 
D) It distinguishes between plasmids that have been transformed and those that have not by black-white screening.

Question 16

What is the natural function of restriction endonucleases?

Accepted Answer

A) regulating bacterial gene expression 
B) DNA recombination in vivo 
C) defending against viruses that infect bacteria 
D) DNA manipulation in vitro 
A) regulating bacterial gene expression 
B) DNA recombination in vivo 
C) defending against viruses that infect bacteria 
D) DNA manipulation in vitro

Question 17

Why is it important to have an antibiotic resistance gene in the cloning plasmid?

Accepted Answer

A) It makes the bacteria resistant to the antibiotic. 
B) It provides a way to sort bacteria that have the gene of interest from the bacteria that have just an "empty" cloning plasmid. 
C) It provides a way for researchers to sort the bacteria that have the cloning plasmid from the bacteria that do not have the cloning plasmid. 
D) It aids scientists in developing new antibiotic treatments. 
A) It makes the bacteria resistant to the antibiotic. 
B) It provides a way to sort bacteria that have the gene of interest from the bacteria that have just an "empty" cloning plasmid. 
C) It provides a way for researchers to sort the bacteria that have the cloning plasmid from the bacteria that do not have the cloning plasmid. 
D) It aids scientists in developing new antibiotic treatments.

Question 18

-According to the table, which enzyme was used to digest a DNA fragment that results in the following sequence? AATTC---------G
G---------CTTAA

Accepted Answer

A) EcoRI 
B) ClaI 
C) BamHI 
D) HindIII 
A) EcoRI 
B) ClaI 
C) BamHI 
D) HindIII

Question 19

cDNA library is the partial collection of cloned cDNAs made from the mRNAs isolated from a cell.

Accepted Answer

A) True 
 B)False

Question 20

Suppose that you generate cDNA libraries for the same cell line under different conditions. Also suppose that when you compare the libraries, you find that some cDNAs in one library are missing from the other library. What is the best explanation for this result?

Accepted Answer

A) One of the cDNA libraries is really a genomic library. 
B) There was an error in generating one of the cDNA libraries, and some of the sequences were not cloned properly. 
C) Even the same cell type will change gene expression as conditions change. The difference in cDNAs tells which genes are expressed in each set of conditions. 
D) The probe used in the Southern blot of the libraries must have been poorly constructed. 
A) One of the cDNA libraries is really a genomic library. 
B) There was an error in generating one of the cDNA libraries, and some of the sequences were not cloned properly. 
C) Even the same cell type will change gene expression as conditions change. The difference in cDNAs tells which genes are expressed in each set of conditions. 
D) The probe used in the Southern blot of the libraries must have been poorly constructed.

Exam 15: Dna Technologies

Match each term with the most appropriate macromolecule being studied or manipulated. -transgenics

Which of the following is characteristic of cDNA?

Match the names of the processes with the descriptions. -You use this technique to produce recombinant DNA.

Match each term with the most appropriate macromolecule being studied or manipulated. -sequencing

What drawback have researchers noted in the cloning of domestic animals?

Gene therapy is a safe, effective, and commercially available treatment for numerous genetic disorders.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -This technique will let you move a gene into a vector for expression in different cells.

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -You would use this to cut DNA at a selected site.

Match each term with the most appropriate macromolecule being studied or manipulated. -agarose gel electrophoresis

Match each term with the most appropriate macromolecule being studied or manipulated. -cDNA library

Explain the ethical concerns with germ-line gene therapy in humans.

Match each term with its definition. -agarose gel electrophoresis

Match each term with its definition. -systems biology

Why is there X-gal used in the cloning medium?

What is the natural function of restriction endonucleases?

Why is it important to have an antibiotic resistance gene in the cloning plasmid?

-According to the table, which enzyme was used to digest a DNA fragment that results in the following sequence? AATTC---------G G---------CTTAA

cDNA library is the partial collection of cloned cDNAs made from the mRNAs isolated from a cell.

Suppose that you generate cDNA libraries for the same cell line under different conditions. Also suppose that when you compare the libraries, you find that some cDNAs in one library are missing from the other library. What is the best explanation for this result?

Light and Life

The Cell: an Overview

Defining Life and Its Origins

Energy and Enzymes

Cell Membranes and Signalling

Cellular Respiration

Photosynthesis

Cell Cycles

Genetic Recombination

Mendel, Genes, and Inheritance

Genes, Chromosomes, and Human Genetics

Dna Structure, Replication, and Organization

Gene Structure and Expression

Control of Gene Expression

Genomes and Proteomes

Evolution: the Development of the Theory

Microevolution: Changes Within Populations

Species and Macroevolution

Understanding the History of Life on Earth

Humans and Evolution

Bacteria and Archaea

Viruses, Viroids, and Prions: Infectious Biological Particles

Protists

Fungi

Plants

Diversity of Animals 1: Sponges, Radiata, Platyhelminthes, and Protostomes

Diversity of Animals 2: Deuterostomes: Vertebrates and Their Closest Relatives

Population Ecology

Population Interactions and Community Ecology

Ecosystems

Conservation of Biodiversity

Putting Selection to Work

Organization of the Plant Body

Transport in Plants

Reproduction and Development in Flowering Plants

Plant Nutrition

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Transport in Animals: the Circulatory System

Reproduction in Animals

Animal Development

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Control

Control of Animal Processes: Neural Integration

Muscles, Skeletons, and Body Movements

Animal Behaviour

Animal Nutrition

Gas Exchange: the Respiratory System

Regulating the Internal Environment

Defences Against Disease

Conservation and Evolutionary Physiology

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