Question 1

What proteins are involved in the circularization of eukaryotic mRNA?

Accepted Answer

The eIF-4E protein that binds to the mRNA cap is also bound to the poly(A) tail through the eIF-4G protein and the poly(A)-binding protein.

Question 2

Chloramphenicol acts by:

Accepted Answer

A)  binding to the peptidase cell-wall enzyme. 
B)  inhibiting peptidyl transferase activity. 
C)  inhibiting protein synthesis. 
D)  binding to fMET tRNA. 
E)  changing the shape of the mRNA. 
A)  binding to the peptidase cell-wall enzyme. 
B)  inhibiting peptidyl transferase activity. 
C)  inhibiting protein synthesis. 
D)  binding to fMET tRNA. 
E)  changing the shape of the mRNA. B

Question 3

Signals that define the beginning and end of protein synthesis are contained in:

Accepted Answer

A)  rRNA. 
B)  tRNA. 
C)  mRNA. 
D)  the ribosome. 
E)  protein. 
A)  rRNA. 
B)  tRNA. 
C)  mRNA. 
D)  the ribosome. 
E)  protein. C

Question 4

What is the mechanism of action of the A fragment of diphtheria toxin?

Accepted Answer

A)  binding the 60S subunit 
B)  cleaving tRNA 
C)  the chemical modification of EF2 
D)  All of the above. 
E)  None of the above. 
A)  binding the 60S subunit 
B)  cleaving tRNA 
C)  the chemical modification of EF2 
D)  All of the above. 
E)  None of the above.

Question 5

In a ribosome, A stands for __________________ and P stands for __________________.

Accepted Answer

The answer of In a ribosome, A stands for __________________...

Question 6

What factor(s) is (are) necessary for the correct placement of the mRNA and initiating tRNA on the ribosome?

Accepted Answer

A)  IF1 
B)  IF2 
C)  IF3 
D)  A and B 
E)  A, B, and C 
A)  IF1 
B)  IF2 
C)  IF3 
D)  A and B 
E)  A, B, and C

Question 7

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ is the first amino acid in bacteria and typically is chemically modified.

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 8

Initiation, elongation, and termination all require factors that are members of the __________________ family.

Accepted Answer

The answer of Initiation, elongation, and termination all require factors...

Question 9

What is the role of the translocon in protein synthesis?

Accepted Answer

The translocon is a multi-subu

Question 10

What is the first step in the elongation cycle?

Accepted Answer

The first step is th

Question 11

In E. coli, the signal sequence that properly aligns the initiation codon in the P site is called the __________________ sequence.

Accepted Answer

The answer of In E. coli, the signal sequence that...

Question 12

Proteins are synthesized in the __________________ direction.

Accepted Answer

N → C, or

Question 13

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-EF-G translocase proteins use the energy of _______________ hydrolysis.

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 14

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The biomolecule _______________ delivers the aminoacyl-tRNA to the "A site" in E. coli.

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 15

What is the final destination of proteins directed to the secretory pathway?

Accepted Answer

Proteins sorted into the secretory pathw

Question 16

What is the direction of translation of mRNA?

Accepted Answer

A)  bidirectional 
B)  5` → 3` 
C)  3` → 5` 
D)  All of the above. 
E)  None of the above. 
A)  bidirectional 
B)  5` → 3` 
C)  3` → 5` 
D)  All of the above. 
E)  None of the above.

Question 17

The first amino acid in bacterial proteins is __________________.

Accepted Answer

The answer of The first amino acid in bacterial proteins...

Question 18

What is the order of the tRNA binding sites on the 70S ribosome with respect to the 5′ → 3′ direction of the mRNA?

Accepted Answer

A)  E P A 
B)  P A E 
C)  A E P 
D)  P E A 
E)  E A P 
A)  E P A 
B)  P A E 
C)  A E P 
D)  P E A 
E)  E A P

Question 19

What role does the amino acid itself play in the accurate placement of the charged tRNA at the codon?

Accepted Answer

None. Experiments prove that o

Question 20

Describe RNAi.

Accepted Answer

RNA interference is a means fo

Exam 40: The Mechanism of Protein Synthesis

What proteins are involved in the circularization of eukaryotic mRNA?

Chloramphenicol acts by:

Signals that define the beginning and end of protein synthesis are contained in:

What is the mechanism of action of the A fragment of diphtheria toxin?

In a ribosome, A stands for __________________ and P stands for __________________.

What factor(s) is (are) necessary for the correct placement of the mRNA and initiating tRNA on the ribosome?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ is the first amino acid in bacteria and typically is chemically modified.

Initiation, elongation, and termination all require factors that are members of the __________________ family.

What is the role of the translocon in protein synthesis?

What is the first step in the elongation cycle?

In E. coli, the signal sequence that properly aligns the initiation codon in the P site is called the __________________ sequence.

Proteins are synthesized in the __________________ direction.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -EF-G translocase proteins use the energy of _______________ hydrolysis.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The biomolecule _______________ delivers the aminoacyl-tRNA to the "A site" in E. coli.

What is the final destination of proteins directed to the secretory pathway?

What is the direction of translation of mRNA?

The first amino acid in bacterial proteins is __________________.

What is the order of the tRNA binding sites on the 70S ribosome with respect to the 5′ → 3′ direction of the mRNA?

What role does the amino acid itself play in the accurate placement of the charged tRNA at the codon?

Describe RNAi.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

Recombinant Dna Techniques

Filters

In a ribosome, A stands for and P stands for .