Exam 36: RNA Synthesis and Regulation in Prokaryotes

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -______________: Sequences deduced from the analysis of many related DNA sequences.

How does the antibiotic rifamycin function mechanistically?

RNA polymerases read the template strand in the _________________ direction.

Many antibiotics, such as rifampicin, function by blocking _________________.

The rate of RNA synthesis in E. coli is approximately ______________ nucleotides per second.

What is a common feature of both protein-dependent and protein-independent termination signals in transcription?

What is the theory about how palindromic RNA polymerase transcription termination signals function?

Describe how RNA is modified.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -______________: A group of genes that are closely related and are produced as a single mRNA.

A σ subunit that is mutated such that the protein binds tightly to DNA with a 10-times-higher affinity than wild-type sequences will likely have what effect?

The Pribnow box:

Adding lactose to E. coli culture results in _________________ of expression of the lac operon.

How is the expression of the lac operon inhibited by the lac repressor?

How does RNA polymerase find the proper binding site?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -An increase in cellular metabolism that inhibits expression of the genes for that metabolism is a process known as ______________.

In E. coli, what other protein(s) is(are) synthesized when β-galactosidase is synthesized?

A mutation in the 3` end of mRNA leads to an increase in AT-rich regions. What is the likely outcome of this change?

Structural diversity of tRNA molecules is caused by:

The _________________ sequence is determined by an alignment of DNA base sequences used to deduce a recurring pattern or motif.

The RNA polymerase can unwind approximately _________________ bases, or about 1.6 turns of B-DNA.