Exam 5: Techniques in Protein Biochemistry

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -____________: The separation of proteins based first on charge then size.

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -____________: A type of purification that is based on the attraction of the protein for a particular chemical group.

When enzymes are purified, the assay is often based on:

Explain the process of immunoprecipitation.

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -____________ can be added prior to gel electrophoresis to denature the proteins.

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -The first step in protein purification from a homogenate is usually ____________.

Proteins that are not catalysts are often assayed using:

Which of the following is true regarding gel-filtration chromatography and PAGE?

A protein that on an SDS-PAGE runs as a single 25kilodalton band runs as a 75kilodalton band on a native gel. Why?

Proteins can be separated from small molecules and ions through a semipermeable membrane by __________________.

Using antibodies as reagents to quantify proteins or other antigens is the basis for the technique called ________________.

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -Sedimentation coefficients are expressed in ____________ units.

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -The subset of gene products actually expressed by the cell is ____________.

Which conditions could cause changes in the proteome of a cell?

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -Proteins with different sedimentation coefficients can be separated by ____________.

Which of the following affects the sedimentation of a particle?

Affinity chromatography:

What is the purpose of determining the specific activity, yield, and purification level of a protein-purification protocol?

Explain the trend of specific activity as a protein is purified.

Use the following to answer questions 1-10: Choose the correct answer from the list below. Not all of the answers will be used. -The ratio of enzyme activity relative to total protein is called ____________.