Exam 6: Basic Concepts of Enzyme Action

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -A reaction that is exergonic will be ____________.

An enzyme which loosely binds substrate will have a __________________ level of specificity.

While some enzymes have very specific substrates, others are more promiscuous. What would you suspect is the reason for this?

In an enzymatic reaction in a test tube, the reaction will eventually reach equilibrium. Why does this not happen in living organisms?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -A tightly bound cofactor might be called a(n) ____________.

The rate of a reaction, or how quickly a reaction will proceed, is best determined by:

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -Enzymes that transfer electrons are called ____________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -Enzymes will decrease the energy of activation but do not change the ____________ of a chemical reaction.

The difference between the standard-state free energy, ΔG°, and the biochemical standard-state free energy is that ΔG°′ refers to the standard free-energy change at __________________.

For the two reactions (a) A → B ∆G°′ = 2 kJ mol−1, and (b) X → Y ∆G°′ = −3.5 kJ mol−1 , which of the following statements is correct?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -____________: The site on the enzyme where the reaction occurs.

The molecular structure that is short-lived and neither substrate nor product is known as the:

The ∆G°′ for the hydrolysis of ATP to ADP + Pi is approximately −31 kJ/mole. Calculate the equilibrium constant for this reaction (R = 8.314 J/°mol) at the cellular temperature of 37°C. If the cellular concentrations of ATP, ADP, and Pi are 8, 1, and 8 mM, respectively, is the above reaction at equilibrium in the cell?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -Enzymes that do not have the required cofactor bound are called ____________.

Multiple dilutions and dialysis of a purified protein results in a loss of enzymatic activity. What might be the cause of this? Assume the structure of the protein is retained.

Organic cofactors are referred to as __________________.

The active site of an enzyme:

Examples of cofactors include:

How is free energy useful for understanding enzyme function?

An enzyme which has been stripped of the small molecules needed for activity is called an __________________.