Exam 39: The Genetic Code

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Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The 5` end of tRNA is _______________.

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F

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -Hydrolysis of _______________ drives the charging of tRNA with amino acids.

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C

How much energy is used to attach the amino acid to the tRNA?

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The equivalents of two ATPs are necessary because acylation is driven by the hydrolysis of the pyrophosphate bond.

Explain why some bases in RNA cannot be involved in base pairs.

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Precision of tRNA-mRNA recognition takes place at the first __________________ base pairs of the codon.

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Describe the subunits of E. coli ribosomes.

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Which amino acid has only one codon triplet?

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Why is it important that transcription and translation are tightly coupled?

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What is the amino acid error rate in protein synthesis? Is this rate logical in evolutionary terms of function?

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Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -In prokaryotic gene expression, _______________ and translation are closely coupled in time and space.

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Protein synthesis takes place on:

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Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The small subunit of the ribosome is _______________.

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Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -An I in the first position of the anticodon indicates that _______________ codons can be read by that tRNA.

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The 3` CCA terminal region of tRNA contains the __________________.

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Class I aminoacyl-tRNA synthetases acylate the __________________ or 3` hydroxyl group of the terminal adenosine of tRNA.

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The activation of a tRNA involves the formation of an __________________ intermediate.

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Where is the attachment site and where is the anticodon loop with respect to the nucleotide sequence and with respect to the L-shaped structure of tRNA?

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Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The amino acid is attached to the tRNA molecule at _______________.

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The ribosome is thought to be:

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What are some of the features common to tRNA molecules?

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