Question 1

The term processivity:

Accepted Answer

A)  indicates the error rate for a polymerase. 
B)  is the deletion of one or more bases in the DNA. 
C)  is specifically the speed of replication. 
D)  is the ability to catalyze many reactions without releasing substrate. 
E)  A, B, and C. 
A)  indicates the error rate for a polymerase. 
B)  is the deletion of one or more bases in the DNA. 
C)  is specifically the speed of replication. 
D)  is the ability to catalyze many reactions without releasing substrate. 
E)  A, B, and C. D

Question 2

Polymerase I has which of the following?

Accepted Answer

A)  a 3` → 5` exonuclease site. 
B)  a 5` → 3` exonuclease site. 
C)  C locus binding site. 
D)  A and B. 
E)  A, B, and C. 
A)  a 3` → 5` exonuclease site. 
B)  a 5` → 3` exonuclease site. 
C)  C locus binding site. 
D)  A and B. 
E)  A, B, and C. D

Question 3

Why is DNA gyrase the target for several different antibiotics?

Accepted Answer

DNA gyrase is the name given to Type II topoisomerase in bacteria. Effective antibiotics are capable of inhibiting the bacterial topoisomerase much more than the Type II topoisomerase in eukaryotic cells.

Question 4

Type ________________ topoisomerase introduces negative supercoils in DNA, while type ________________ topoisomerase relaxes supercoil structures.

Accepted Answer

The answer of Type ________________ topoisomerase introduces negative supercoils in...

Question 5

What is a processive polymerase enzyme versus a distributive polymerase enzyme?

Accepted Answer

Processive enzymes catalyze mu

Question 6

Choose the correct answer from the list below. Not all of the answers will be used.
-The small DNA synthesized on the lagging strand are called _______________ fragments.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 7

How are single-stranded regions of DNA maintained?

Accepted Answer

Single-stranded bind

Question 8

Proofreading by DNA polymerase increases accuracy by a factor of _________________ times.

Accepted Answer

The answer of Proofreading by DNA polymerase increases accuracy by...

Question 9

What are the minimal requirements for DNA replication?

Accepted Answer

DNA replication requires DNA t

Question 10

How is replication specificity dictated?

Accepted Answer

A)  Watson-Crick hydrogen bonding must occur. 
B)  Enzyme interactions with the DNA act as a "ruler" to determine whether the properly spaced base pair has been formed. 
C)  The bond is broken and reformed to ensure its accurate placement at each base pair. 
D)  A and B. 
E)  A, B, and C. 
A)  Watson-Crick hydrogen bonding must occur. 
B)  Enzyme interactions with the DNA act as a "ruler" to determine whether the properly spaced base pair has been formed. 
C)  The bond is broken and reformed to ensure its accurate placement at each base pair. 
D)  A and B. 
E)  A, B, and C.

Question 11

Replication of telomers Involves:

Accepted Answer

A)  an RNA-dependent DNA polymerase. 
B)  GC capping. 
C)  looping of the DNA ends. 
D)  helicase-dependent ATP melting of the strands. 
E)  None of the above. 
A)  an RNA-dependent DNA polymerase. 
B)  GC capping. 
C)  looping of the DNA ends. 
D)  helicase-dependent ATP melting of the strands. 
E)  None of the above.

Question 12

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ is critical in the formation of a telomere.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 13

The DNA strand that is replicated continuously is known as the __________________ strand.

Accepted Answer

The answer of The DNA strand that is replicated continuously...

Question 14

Choose the correct answer from the list below. Not all of the answers will be used.
-The type of topoisomerase that can introduce supercoils is _______________.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 15

The observed error rate in DNA replication is:

Accepted Answer

A)  1 per 106 nucleotides. 
B)  1 per 104 nucleotides. 
C)  1 per 108 nucleotides. 
D)  1 per 1015 nucleotides. 
E)  None of the above. 
A)  1 per 106 nucleotides. 
B)  1 per 104 nucleotides. 
C)  1 per 108 nucleotides. 
D)  1 per 1015 nucleotides. 
E)  None of the above.

Question 16

The dimeric β2 subunit of DNA polymerase III acts as a(n) __________________.

Accepted Answer

The answer of The dimeric β2 subunit of DNA polymerase...

Question 17

In eukaryotes, each origin of replication represents a replication unit, or __________________.

Accepted Answer

The answer of In eukaryotes, each origin of replication represents...

Question 18

The ends of the eukaryotic chromosomes are called:

Accepted Answer

A)  pyrimidine caps. 
B)  telomeres. 
C)  G-rich ends. 
D)  replicon ends. 
E)  None of the above. 
A)  pyrimidine caps. 
B)  telomeres. 
C)  G-rich ends. 
D)  replicon ends. 
E)  None of the above.

Question 19

Choose the correct answer from the list below. Not all of the answers will be used.
-The ends of chromosomes are called _______________.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 20

The accuracy for DNA polymerase depends on the proper formation of a __________________.

Accepted Answer

Watson-Cri

Exam 34: DNA Replication

The term processivity:

Polymerase I has which of the following?

Why is DNA gyrase the target for several different antibiotics?

Type ________________ topoisomerase introduces negative supercoils in DNA, while type ________________ topoisomerase relaxes supercoil structures.

What is a processive polymerase enzyme versus a distributive polymerase enzyme?

Choose the correct answer from the list below. Not all of the answers will be used. -The small DNA synthesized on the lagging strand are called _______________ fragments.

How are single-stranded regions of DNA maintained?

Proofreading by DNA polymerase increases accuracy by a factor of _________________ times.

What are the minimal requirements for DNA replication?

How is replication specificity dictated?

Replication of telomers Involves:

Choose the correct answer from the list below. Not all of the answers will be used. -_______________ is critical in the formation of a telomere.

The DNA strand that is replicated continuously is known as the __________________ strand.

Choose the correct answer from the list below. Not all of the answers will be used. -The type of topoisomerase that can introduce supercoils is _______________.

The observed error rate in DNA replication is:

The dimeric β2 subunit of DNA polymerase III acts as a(n) __________________.

In eukaryotes, each origin of replication represents a replication unit, or __________________.

The ends of the eukaryotic chromosomes are called:

Choose the correct answer from the list below. Not all of the answers will be used. -The ends of chromosomes are called _______________.

The accuracy for DNA polymerase depends on the proper formation of a __________________.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

Recombinant DNA Techniques

Filters

Type topoisomerase introduces negative supercoils in DNA, while type topoisomerase relaxes supercoil structures.