Exam 5: Techniques in Protein Biochemistry

Choose the correct answer from the list below. Not all of the answers will be used. -The ratio of enzyme activity relative to total protein is called ____________.

In the Edman procedure for peptide sequence, phenyl isothiocyanate is used to selectively derivitize the __________________ residue, which is then removed as a PTH derivative.

Which types of molecules can serve as antigens?

How do gel-filtration and ion-exchange chromatography differ?

Choose the correct answer from the list below. Not all of the answers will be used. -Sedimentation coefficients are expressed in ____________ units.

Which of the following affects the sedimentation of a particle?

What is the purpose of determining the specific activity, yield, and purification level of a protein-purification protocol?

Which technique cannot be used for quantitative analysis?

Explain the process of immunoprecipitation.

Choose the correct answer from the list below. Not all of the answers will be used. -The first step in protein purification from a homogenate is usually ____________.

Start with a technique used on a complex mixture of proteins, such as a cell lysate, through a series of steps to a pure protein.

How can a protein's isoelectric point be used in protein purification?

What type of information can be obtained from gradient centrifugation?

Polypeptides can be fragmented into smaller peptides by cleavage with chymotrypsin, which hydrolyzes the peptide bond at the C-terminal side of ____, ______, and ______ residues.

Choose the correct answer from the list below. Not all of the answers will be used. -____________ can be added prior to gel electrophoresis to denature the proteins.

Which conditions could cause changes in the proteome of a cell?

Why is an assay necessary for protein-purification studies?

Why is there a need for different digestion tools when fingerprinting a protein?

Using antibodies as reagents to quantify proteins or other antigens is the basis for the technique called ________________.

Two-dimensional electrophoresis is a combination of which two techniques?