Question 1

The Sanger technique for sequencing DNA involves the use of _____________________ nucleotide analogs that terminate chain elongation.

Accepted Answer

2`,3`-dideoxy

Question 2

The specificity or stringency of a PCR reaction can be controlled by altering the reaction:

Accepted Answer

A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  All of the above. 
E)  None of the above. 
A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  All of the above. 
E)  None of the above. C

Question 3

Choose the correct answer from the list below. Not all of the answers will be used.
-The type of vector used for synthesis of protein is _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction D

Question 4

Choose the correct answer from the list below. Not all of the answers will be used.
-Instead of radioactivity, current DNA sequencing commonly uses _______________ base analogues.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 5

PCR is the abbreviation for _____________________, which is an in vitro technique used to make multiple copies of a DNA molecule.

Accepted Answer

polymerase

Question 6

Why are monoclonal antibodies more useful than polyclonal antibodies?

Accepted Answer

Monoclonal antibodies are more specific,

Question 7

What is a DNA probe?

Accepted Answer

A DNA probe is used to identify specific

Question 8

Choose the correct answer from the list below. Not all of the answers will be used.
-DNA sequences are amplified by the _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 9

How does quantitative PCR differ from nonquantitative PCR?

Accepted Answer

In nonquantitative PCR, a gene is amplif

Question 10

How is gene disruption used to determine the function of a gene?

Accepted Answer

If a gene is completely disrupted (a pro

Question 11

Bacterial plasmid DNA and bacteriophage DNA are commonly used _____________________ to introduce foreign DNA into a bacterium.

Accepted Answer

The answer of Bacterial plasmid DNA and bacteriophage DNA are...

Question 12

Explain the basis of the polymerase chain reaction.

Accepted Answer

Using primers that flank the sequence of

Question 13

What is a transcriptome?

Accepted Answer

A transcriptome is the pattern

Question 14

Complementary DNA (cDNA) is formed by the action of reverse transcriptase on _____________________.

Accepted Answer

The answer of Complementary DNA (cDNA) is formed by the...

Question 15

Design a potential DNA-restriction enzyme site. Show both strands.

Accepted Answer

Any palindromic site

Question 16

Reagents necessary for sequencing by chain termination include:

Accepted Answer

A)  template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxynucleotide analogs, DNA polymerase, and radioactive probe. 
B)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and DNA polymerase. 
C)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and RNA polymerase. 
D)  All of the above. 
E)  None of the above 
A)  template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxynucleotide analogs, DNA polymerase, and radioactive probe. 
B)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and DNA polymerase. 
C)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and RNA polymerase. 
D)  All of the above. 
E)  None of the above

Question 17

A number of tools are critical to gene exploration. Name at least four.

Accepted Answer

Tools include restriction-enzy

Question 18

Describe two ways PCR can be used in medical diagnosis.

Accepted Answer

PCR can be used to detect whet

Question 19

How is a single gene of interest identified on a plate containing many different library clones?

Accepted Answer

By using a probe specific for the DNA of

Question 20

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________is a method for analyzing the expression of thousands of genes.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Exam 41: Recombinant DNA Techniques

The Sanger technique for sequencing DNA involves the use of _____________________ nucleotide analogs that terminate chain elongation.

The specificity or stringency of a PCR reaction can be controlled by altering the reaction:

Choose the correct answer from the list below. Not all of the answers will be used. -The type of vector used for synthesis of protein is _______________.

Choose the correct answer from the list below. Not all of the answers will be used. -Instead of radioactivity, current DNA sequencing commonly uses _______________ base analogues.

PCR is the abbreviation for _____________________, which is an in vitro technique used to make multiple copies of a DNA molecule.

Why are monoclonal antibodies more useful than polyclonal antibodies?

What is a DNA probe?

Choose the correct answer from the list below. Not all of the answers will be used. -DNA sequences are amplified by the _______________.

How does quantitative PCR differ from nonquantitative PCR?

How is gene disruption used to determine the function of a gene?

Bacterial plasmid DNA and bacteriophage DNA are commonly used _____________________ to introduce foreign DNA into a bacterium.

Explain the basis of the polymerase chain reaction.

What is a transcriptome?

Complementary DNA (cDNA) is formed by the action of reverse transcriptase on _____________________.

Design a potential DNA-restriction enzyme site. Show both strands.

Reagents necessary for sequencing by chain termination include:

A number of tools are critical to gene exploration. Name at least four.

Describe two ways PCR can be used in medical diagnosis.

How is a single gene of interest identified on a plate containing many different library clones?

Choose the correct answer from the list below. Not all of the answers will be used. -_______________is a method for analyzing the expression of thousands of genes.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

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