Exam 17: Biotechnology

Which of the following two enzymes are needed to construct a cDNA library?

The PCR technique requires a DNA polymerase from an organism that can endure high heat, such as Thermus aquaticus.What step of the protocol makes the heat-stability most necessary?

An immunoassay was developed to rapidly diagnose patients infected with an emerging virus.However, the researchers discovered that some individuals were producing false negatives when tested, despite being infected with the virus.What might explain this?

All of the following involve molecular biology techniques except

A farmer is growing glyphosate-resistant transgenic soybeans.What are you unlikely to see in those fields during the growing season?

You want to insert your favorite gene, which has been digested with Ncol endonuclease on both ends, into a vector.Ncol recognizes the DNA sequence C*CATGG.The vector you want to use contains many endonuclease recognition sites, but does not include an Ncol site.Which restriction endonuclease would be the most suitable replacement to digest the vector?

An enzyme that has been isolated from retroviruses and is used to generate cDNA is

The _________ from Agrobacterium tumefaciens is used in genetic engineering involving crop plant genes.

You have discovered a very small amount of DNA from an ancient organism that you want to save and study.What is the very first thing you should do to allow you to study this DNA in the lab?

The most common way to follow bacterial transformation with a plasmid is by

A key difference between transforming plant cells and human cells is

The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the _______ fate of organisms.

Imagine that a very unusual animal species had the ability to make its own aromatic amino acids using a 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase enzyme similar to that of plants.What would happen if this animal foraged in a field of glyphosate-resistant plants?

Investigators have found a new species bacteria that lives under both aerobic and anaerobic conditions.They hypothesize that different genes are expressed in each condition.What experiment could test this hypothesis and identify important genes?

Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium

Gel ________ is a process that separates DNA or protein fragments according to their size, by causing them to migrate within a gel in response to an electric field.

A certain wastewater treatment plant uses aerobic bacteria, anaerobic bacteria, and archaebacteria during secondary treatment of sewage.Unfortunately, during a bad blizzard the aerated tanks froze, killing the microbes inside.What is likely to result?

Bacterial plasmids and artificial chromosomes are used as ______ to insert foreign DNA into host cells and create recombinant genomes.

Technique used to amplify a desired piece of DNA.

Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)