Question 1

Describe the difference between the concerted and the sequential model of allosteric regulation.

Accepted Answer

The concerted model describes a situatio

Question 2

What is the Michaelis-Menten equation? Define all parameters.

Accepted Answer

V0 = Vmax(S/(S + KM))
Initial

Question 3

Would you expect the order of substrate binding to be critical for enzyme catalysis?

Accepted Answer

Yes, in some cases. For example, in ping

Question 4

______________ is directly dependent on enzyme concentration.

Accepted Answer

The answer of ______________ is directly dependent on enzyme concentration....

Question 5

Which has the strongest affinity when k−1 is greater than k2?

Accepted Answer

A)  150 mM 
B)  0.15 mM 
C)  150  μM 
D)  1.5 nM 
E)  15,000 pM 
A)  150 mM 
B)  0.15 mM 
C)  150  μM 
D)  1.5 nM 
E)  15,000 pM

Question 6

Choose the correct answer from the list below. Not all of the answers will be used.
-A reaction that is directly proportional to the concentration of reactant is a(n) ______________.

Accepted Answer

A) first-order reaction 
B) second-order reaction 
C) metabolism 
D) ensemble 
E) biomolecular
F)turnover number
G)Michaelis-Menten
H)equilibrium
I)bsequential
J)kinetics
K)initial velocity
L)allosteric
M)ping-pong 
A) first-order reaction 
B) second-order reaction 
C) metabolism 
D) ensemble 
E) biomolecular
F)turnover number
G)Michaelis-Menten
H)equilibrium
I)bsequential
J)kinetics
K)initial velocity
L)allosteric
M)ping-pong

Question 7

Choose the correct answer from the list below. Not all of the answers will be used.
-An enzyme's affinity for its substrate is measured by the _______________ constant.

Accepted Answer

A) first-order reaction 
B) second-order reaction 
C) metabolism 
D) ensemble 
E) biomolecular
F)turnover number
G)Michaelis-Menten
H)equilibrium
I)bsequential
J)kinetics
K)initial velocity
L)allosteric
M)ping-pong 
A) first-order reaction 
B) second-order reaction 
C) metabolism 
D) ensemble 
E) biomolecular
F)turnover number
G)Michaelis-Menten
H)equilibrium
I)bsequential
J)kinetics
K)initial velocity
L)allosteric
M)ping-pong

Question 8

How does the sequential model differ from the concerted model for allosteric enzymes?

Accepted Answer

The concerted model does not allow for a

Question 9

The model describing allosteric regulation that requires all subunits to be in the same state is called the:

Accepted Answer

A)  concerted model. 
B)  syncopated model. 
C)  cooperative model. 
D)  equilibrium model. 
E)  None of the above. 
A)  concerted model. 
B)  syncopated model. 
C)  cooperative model. 
D)  equilibrium model. 
E)  None of the above.

Question 10

Choose the correct answer from the list below. Not all of the answers will be used.
-A reaction with two substrates is considered a(n) _______________ reaction.

Accepted Answer

A) first-order reaction 
B) second-order reaction 
C) metabolism 
D) ensemble 
E) biomolecular
F)turnover number
G)Michaelis-Menten
H)equilibrium
I)bsequential
J)kinetics
K)initial velocity
L)allosteric
M)ping-pong 
A) first-order reaction 
B) second-order reaction 
C) metabolism 
D) ensemble 
E) biomolecular
F)turnover number
G)Michaelis-Menten
H)equilibrium
I)bsequential
J)kinetics
K)initial velocity
L)allosteric
M)ping-pong

Question 11

What does Vmax indicate?

Accepted Answer

The maximum velocity or rate o

Question 12

The formula V0 = $\begin{array}{l}
V _ { 0 } = V _ { \max }\
\end{array}$$\frac{[S] }{\mathrm{S}]+K_{\mathrm{M}}}$ , indicates the relationship between

Accepted Answer

A)  the enzyme activity and the equilibrium constant. 
B)  the rate of a catalyzed reaction and the equilibrium constant. 
C)  enzyme activity as a function of substrate concentration. 
D)  All of the above. 
E)  None of the above. 
A)  the enzyme activity and the equilibrium constant. 
B)  the rate of a catalyzed reaction and the equilibrium constant. 
C)  enzyme activity as a function of substrate concentration. 
D)  All of the above. 
E)  None of the above.

Question 13

The KM is:

Accepted Answer

A)  equal to the product concentration at initial reaction conditions. 
B)  equal to the substrate concentration when the reaction rate is half its maximal value. 
C)  proportional to the standard free energy. 
D)  All of the above. 
E)  None of the above. 
A)  equal to the product concentration at initial reaction conditions. 
B)  equal to the substrate concentration when the reaction rate is half its maximal value. 
C)  proportional to the standard free energy. 
D)  All of the above. 
E)  None of the above.

Question 14

Allosteric modifiers alter the equilibria between:

Accepted Answer

A)  the ES state. 
B)  the R and T forms of a protein. 
C)  the forward and reverse reaction rate. 
D)  the formation of a product and its reverse reaction. 
E)  All of the above. 
A)  the ES state. 
B)  the R and T forms of a protein. 
C)  the forward and reverse reaction rate. 
D)  the formation of a product and its reverse reaction. 
E)  All of the above.

Question 15

A critical feature of the Michaelis-Menten model of enzyme catalysis is:

Accepted Answer

A)  increasing the probability of product formation. 
B)  shifting the reaction equilibrium. 
C)  the formation of an ES complex. 
D)  All of the above. 
E)  None of the above. 
A)  increasing the probability of product formation. 
B)  shifting the reaction equilibrium. 
C)  the formation of an ES complex. 
D)  All of the above. 
E)  None of the above.

Question 16

The ______________ rule states that all subunits in an allosteric enzyme must be in either the T or the R state; there can not be hybrids.

Accepted Answer

The answer of The ______________ rule states that all subunits...

Question 17

What value of [S], as a fraction of KM, is required to obtain 20% Vmax? [S] equals:

Accepted Answer

A)  0.2 KM 
B)  0.25 KM 
C)  0.5 KM 
D)  0.75 KM 
E)  0.8 KM 
A)  0.2 KM 
B)  0.25 KM 
C)  0.5 KM 
D)  0.75 KM 
E)  0.8 KM

Question 18

What is the turnover number for an enzyme and what does this value tell us about the enzyme?

Accepted Answer

The rate of reaction and dissociation of

Question 19

The Michaelis-Menten model assumes that ______________ is the rate constant ignored because producthas not accumulated.

Accepted Answer

The answer of The Michaelis-Menten model assumes that ______________ is...

Question 20

An enzyme will be most sensitive to changes in cellular concentration when the concentration is ______________.

Accepted Answer

The answer of An enzyme will be most sensitive to...

Exam 7: Kinetics and Regulation

Describe the difference between the concerted and the sequential model of allosteric regulation.

What is the Michaelis-Menten equation? Define all parameters.

Would you expect the order of substrate binding to be critical for enzyme catalysis?

______________ is directly dependent on enzyme concentration.

Which has the strongest affinity when k−1 is greater than k2?

Choose the correct answer from the list below. Not all of the answers will be used. -A reaction that is directly proportional to the concentration of reactant is a(n) ______________.

Choose the correct answer from the list below. Not all of the answers will be used. -An enzyme's affinity for its substrate is measured by the _______________ constant.

How does the sequential model differ from the concerted model for allosteric enzymes?

The model describing allosteric regulation that requires all subunits to be in the same state is called the:

Choose the correct answer from the list below. Not all of the answers will be used. -A reaction with two substrates is considered a(n) _______________ reaction.

What does Vmax indicate?

The formula V0 = = [S]S]+KM\frac{[S] }{\mathrm{S}]+K_{\mathrm{M}}}S]+KM​[S]​ , indicates the relationship between

The KM is:

Allosteric modifiers alter the equilibria between:

A critical feature of the Michaelis-Menten model of enzyme catalysis is:

The ______________ rule states that all subunits in an allosteric enzyme must be in either the T or the R state; there can not be hybrids.

What value of [S], as a fraction of KM, is required to obtain 20% Vmax? [S] equals:

What is the turnover number for an enzyme and what does this value tell us about the enzyme?

The Michaelis-Menten model assumes that ______________ is the rate constant ignored because producthas not accumulated.

An enzyme will be most sensitive to changes in cellular concentration when the concentration is ______________.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

Recombinant DNA Techniques

Filters

The formula V₀ = = $\frac{[S] }{\mathrm{S}]+K_{\mathrm{M}}}$ , indicates the relationship between

The K_M is:

What value of [S], as a fraction of K_M, is required to obtain 20% V_max? [S] equals: