Question 1

All of the following concerning agglutination reactions are true EXCEPT

Accepted Answer

A) they are used only with red blood cells. 
B) they only detect molecules on the surface of cells. 
C) they require a high degree of expertise to interpret. 
D) they should be done with titrations of antibodies. 
E) All of the answers are true. 
A) they are used only with red blood cells. 
B) they only detect molecules on the surface of cells. 
C) they require a high degree of expertise to interpret. 
D) they should be done with titrations of antibodies. 
E) All of the answers are true. C

Question 2

Multiphoton microscopy is a variation on what type of technique?

Accepted Answer

A) Confocal microscopy 
B) FACS 
C) MACS 
D) Electron microscopy 
E) Immunohistochemistry 
A) Confocal microscopy 
B) FACS 
C) MACS 
D) Electron microscopy 
E) Immunohistochemistry A

Question 3

Hemagglutination inhibition reactions are used to detect

Accepted Answer

A) responses to bacteria. 
B) responses to viruses. 
C) proteins on the surface of red blood cells. 
D) All of the answers are correct. 
E) None of the answers are correct. 
A) responses to bacteria. 
B) responses to viruses. 
C) proteins on the surface of red blood cells. 
D) All of the answers are correct. 
E) None of the answers are correct. B

Question 4

Imagine you want to study Promoter X, which controls many of the genes in B cells that you believe are important for controlling viral infections.You want to generate a transgenic mouse line with a mutation in this promoter that makes it expressed at low levels.Unfortunately, after all of your work, you learn that your mutation is "embryonic lethal." Describe the strategies/techniques that could be used to circumvent this problem.

Accepted Answer

One technique you could use is the Cre/l

Question 5

In chromium release assays, or more recently in CFSE release assays, cells that die release the marker if they

Accepted Answer

A) die by apoptosis only. 
B) die by necrosis only. 
C) die by either apoptosis or necrosis. 
D) divide before dying. 
E) replicate DNA before dying. 
A) die by apoptosis only. 
B) die by necrosis only. 
C) die by either apoptosis or necrosis. 
D) divide before dying. 
E) replicate DNA before dying.

Question 6

Which of the following is NOT required to generate a B-cell hybridoma?

Accepted Answer

A) Myeloma cell line 
B) Primary plasma cell 
C) Chemical fusogens 
D) HGPRT selection 
E) None of the answers are required to generate a B-cell hybdridoma. 
A) Myeloma cell line 
B) Primary plasma cell 
C) Chemical fusogens 
D) HGPRT selection 
E) None of the answers are required to generate a B-cell hybdridoma.

Question 7

ELISPOT assays

Accepted Answer

A) are modifications of Western blotting. 
B) are used to detect individual cells. 
C) involve the use of "capture" antibodies. 
D) both involve the use of "capture" antibodies and are modifications of Western blotting. 
E) both involve the use of "capture" antibodies and are used to detect individual cells. 
A) are modifications of Western blotting. 
B) are used to detect individual cells. 
C) involve the use of "capture" antibodies. 
D) both involve the use of "capture" antibodies and are modifications of Western blotting. 
E) both involve the use of "capture" antibodies and are used to detect individual cells.

Question 8

Carboxyfluorescein succinimidyl ester (CFSE) can be used to track cell division because

Accepted Answer

A) after pulsing the cells with it, dividing cells will incorporate more into their DNA. 
B) after pulsing cells with it, dividing cells will incorporate more into their proteins. 
C) its intensity is cut in half, approximately, when DNA replicates. 
D) its intensity is cut in half, approximately, when the cell divides. 
E) None of the answers are correct. 
A) after pulsing the cells with it, dividing cells will incorporate more into their DNA. 
B) after pulsing cells with it, dividing cells will incorporate more into their proteins. 
C) its intensity is cut in half, approximately, when DNA replicates. 
D) its intensity is cut in half, approximately, when the cell divides. 
E) None of the answers are correct.

Question 9

Immunoprecipitation does NOT

Accepted Answer

A) allow characterization of molecules bound to cells. 
B) take place in gel matrices. 
C) work well in solution. 
D) require the use of a monoclonal antibody. 
E) All of the answers are characteristics of immunoprecipitation. 
A) allow characterization of molecules bound to cells. 
B) take place in gel matrices. 
C) work well in solution. 
D) require the use of a monoclonal antibody. 
E) All of the answers are characteristics of immunoprecipitation.

Question 10

The Cre/lox system is NOT able to

Accepted Answer

A) delete a gene in a specific tissue type. 
B) delete a gene when a drug is introduced. 
C) express a gene only in specific tissues. 
D) enable study of embryonic lethal gene alterations. 
E) All of the answers are correct. 
A) delete a gene in a specific tissue type. 
B) delete a gene when a drug is introduced. 
C) express a gene only in specific tissues. 
D) enable study of embryonic lethal gene alterations. 
E) All of the answers are correct.

Question 11

The major distinction between FACS and MACS is that

Accepted Answer

A) MACS is not as precise as FACS. 
B) MACS does not use a fluorescent marker. 
C) MACS can be used with living cells. 
D) MACS can be used for intracellular staining. 
E) All of the answers are correct. 
A) MACS is not as precise as FACS. 
B) MACS does not use a fluorescent marker. 
C) MACS can be used with living cells. 
D) MACS can be used for intracellular staining. 
E) All of the answers are correct.

Question 12

Which of the following does NOT describe equilibrium dialysis?

Accepted Answer

A) It is used to quantitate binding between antibody and antigen. 
B) It depends on the ligand being able to cross a semipermeable membrane. 
C) It measures the amount of antibody-bound ligand compared to unbound ligand. 
D) It requires an enzyme amplification step. 
E) All of the answers describe equilibrium dialysis. 
A) It is used to quantitate binding between antibody and antigen. 
B) It depends on the ligand being able to cross a semipermeable membrane. 
C) It measures the amount of antibody-bound ligand compared to unbound ligand. 
D) It requires an enzyme amplification step. 
E) All of the answers describe equilibrium dialysis.

Question 13

Polyclonal antibodies differ from monoclonal antibodies in all ways EXCEPT which of the following?

Accepted Answer

A) They generally have fewer cross-reactivities. 
B) They are generally better for immunoprecipitation. 
C) They generally have lower affinity. 
D) They can vary from preparation to preparation. 
E) All of the answers are ways in which they differ. 
A) They generally have fewer cross-reactivities. 
B) They are generally better for immunoprecipitation. 
C) They generally have lower affinity. 
D) They can vary from preparation to preparation. 
E) All of the answers are ways in which they differ.

Question 14

What simpler technique from immunology is the basis for modern flow cytometry, and how does it improve on the earlier assays? Explain.

Accepted Answer

Flow cytometry (and FACS) is based on th

Question 15

Immunofluorescence-based imaging

Accepted Answer

A) allows fine-scale visualization of subcellular localization. 
B) has been enhanced by the availability of multiple dyes. 
C) has been simplified by genetic engineering to tag GFP onto target proteins. 
D) can take advantage of molecules that do not need antibodies to bind targets. 
E) All of the answers are correct. 
A) allows fine-scale visualization of subcellular localization. 
B) has been enhanced by the availability of multiple dyes. 
C) has been simplified by genetic engineering to tag GFP onto target proteins. 
D) can take advantage of molecules that do not need antibodies to bind targets. 
E) All of the answers are correct.

Question 16

Compare and contrast indirect and sandwich ELISA assays.Which requires multiple types of antibodies against the target antigen? Explain.

Accepted Answer

ELISAs test for the presence of antibodi

Question 17

List and evaluate three ways to measure cell death experimentally.

Accepted Answer

The first assay to do this reliably was

Question 18

Immunohistochemistry and immunocytochemistry differ from each other in that

Accepted Answer

A) immunocytochemistry analyzes tissue sections. 
B) immunohistochemistry analyzes individual cells. 
C) immunohistochemistry uses electron-gold microscopy. 
D) Immunocytochemistry analyzes individual cells. 
E) None of the answers are correct. 
A) immunocytochemistry analyzes tissue sections. 
B) immunohistochemistry analyzes individual cells. 
C) immunohistochemistry uses electron-gold microscopy. 
D) Immunocytochemistry analyzes individual cells. 
E) None of the answers are correct.

Question 19

Annexin-V staining of cells identifies

Accepted Answer

A) a failure of membrane integrity. 
B) broken ends of chromosomes produced during DNA fragmentation. 
C) necrotic but not apoptotic cells. 
D) both apoptotic and necrotic cells. 
E) a breakdown of membrane asymmetry. 
A) a failure of membrane integrity. 
B) broken ends of chromosomes produced during DNA fragmentation. 
C) necrotic but not apoptotic cells. 
D) both apoptotic and necrotic cells. 
E) a breakdown of membrane asymmetry.

Question 20

The use of inbred lines of mice

Accepted Answer

A) reduces genetic heterogeneity. 
B) reduces experimental variables. 
C) increases similarity among individuals. 
D) leads to syngeneic animals. 
E) All of the answers are correct. 
A) reduces genetic heterogeneity. 
B) reduces experimental variables. 
C) increases similarity among individuals. 
D) leads to syngeneic animals. 
E) All of the answers are correct.

All of the following concerning agglutination reactions are true EXCEPT

Multiphoton microscopy is a variation on what type of technique?

Hemagglutination inhibition reactions are used to detect

In chromium release assays, or more recently in CFSE release assays, cells that die release the marker if they

Which of the following is NOT required to generate a B-cell hybridoma?

ELISPOT assays

Carboxyfluorescein succinimidyl ester (CFSE) can be used to track cell division because

Immunoprecipitation does NOT

The Cre/lox system is NOT able to

The major distinction between FACS and MACS is that

Which of the following does NOT describe equilibrium dialysis?

Polyclonal antibodies differ from monoclonal antibodies in all ways EXCEPT which of the following?

What simpler technique from immunology is the basis for modern flow cytometry, and how does it improve on the earlier assays? Explain.

Immunofluorescence-based imaging

Compare and contrast indirect and sandwich ELISA assays.Which requires multiple types of antibodies against the target antigen? Explain.

List and evaluate three ways to measure cell death experimentally.

Immunohistochemistry and immunocytochemistry differ from each other in that

Annexin-V staining of cells identifies

The use of inbred lines of mice

Overview of the Immune System

Cells, Organs, and Microenvironments of the Immune System

Recognition and Response

Innate Immunity

The Complement System

The Organization and Expression of Lymphocyte Receptor Genes

The Major Histocompatibility Complex and Antigen Presentation

T-Cell Development

B-Cell Development

T-Cell Activation, Differentiation, and Memory

B-Cell Activation, Differentiation, and Memory

Effector Responses: Cell- and Antibody-Mediated Immunity

Mucosal Immunity and the Microbiome

The Immune Response in Time and Space

Allergy, Hypersensitivities, and Chronic Inflammation

Tolerance, Autoimmunity, and Transplantation

Infectious Diseases and Vaccines

Immunodeficiency Disorders

Cancer and the Immune System

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Exam 20: Experimental Methods and Systems

All of the following concerning agglutination reactions are true EXCEPT

Multiphoton microscopy is a variation on what type of technique?

Hemagglutination inhibition reactions are used to detect

In chromium release assays, or more recently in CFSE release assays, cells that die release the marker if they

Which of the following is NOT required to generate a B-cell hybridoma?

ELISPOT assays

Carboxyfluorescein succinimidyl ester (CFSE) can be used to track cell division because

Immunoprecipitation does NOT

The Cre/lox system is NOT able to

The major distinction between FACS and MACS is that

Which of the following does NOT describe equilibrium dialysis?

Polyclonal antibodies differ from monoclonal antibodies in all ways EXCEPT which of the following?

What simpler technique from immunology is the basis for modern flow cytometry, and how does it improve on the earlier assays? Explain.

Immunofluorescence-based imaging

Compare and contrast indirect and sandwich ELISA assays.Which requires multiple types of antibodies against the target antigen? Explain.

List and evaluate three ways to measure cell death experimentally.

Immunohistochemistry and immunocytochemistry differ from each other in that

Annexin-V staining of cells identifies

The use of inbred lines of mice

Overview of the Immune System

Cells, Organs, and Microenvironments of the Immune System

Recognition and Response

Innate Immunity

The Complement System

The Organization and Expression of Lymphocyte Receptor Genes

The Major Histocompatibility Complex and Antigen Presentation

T-Cell Development

B-Cell Development

T-Cell Activation, Differentiation, and Memory

B-Cell Activation, Differentiation, and Memory

Effector Responses: Cell- and Antibody-Mediated Immunity

Mucosal Immunity and the Microbiome

The Immune Response in Time and Space

Allergy, Hypersensitivities, and Chronic Inflammation

Tolerance, Autoimmunity, and Transplantation

Infectious Diseases and Vaccines

Immunodeficiency Disorders

Cancer and the Immune System

Filters