Exam 6: The Organization and Expression of Lymphocyte Receptor Genes

Several proteins must work in a coordinating fashion to achieve V(D)J recombination.Which is responsible for adding nontemplated nucleotides to the V-D and D-J joints of Ig heavy chains?

Which of the following TCR gene variable regions possesses a "D" segment?

What cleaves interstrand hairpin in V(D)J coding regions?

V(D)J recombination involves a significant number of proteins, working together in a coordinated fashion.Can you think of two potential reasons why so many proteins are involved?

Which protein-encoding genes, although present in genomic DNA, are uniquely expressed by lymphocytes?

At which point in the variable segments does the RAG1/2 complex introduce a single-strand break into the DNA during somatic rearrangement?

Consider the following statement from the text, "Immunologists had to face the fact that there simply was not enough DNA to encode all the relevant B-cell receptors, using the one-gene, one-polypeptide chain rule." In one sentence, could you explain, in your own words, what this statement means to a fellow student in your immunology course?

What binds cut DNA and retains it in protein-DNA complex?

Which of the following BEST encapsulates the findings of the Hozumi and Tonegawa experiments?

Recognition spacer sequences are separated by how many base pairs?

Which of the following accounts for allelic exclusion in rearrangement within the IgH locus?

Which of the following components that are essential for rearrangement of the IgH and IgL loci are NOT utilized in rearrangement of the TCR loci?

Which is part of the mature BCR complex but is NOT part of the pre-BCR complex?

Which of the following is capable of undergoing successive rearrangements to prevent the formation of autoreactive lymphocyte receptors?

Imagine a lymphocyte is actively undergoing V(D)J recombination.V(D)J recombination has reached a stage where alleles are being excluded from expression.Within the nucleus, where would you expect to find the excluded alleles, and why?

If a given B cell is found to be potentially reactive to self-antigens, can the cell make an effort to correct that flaw, or is it destined for eventual removal by apoptosis?

Deficiency in RAG1 and/or RAG2 can affect the proper development of which of the following cell types, resulting in decreased numbers of circulating cells of those types?

Which of the following IgH chains is NOT produced as a result of CSR?

Taking into consideration the length of bases between recombination sequences, can you describe the functional significance of the lengths?

What introduces nucleotides in a template-independent manner to free 3' OH created upon cleavage of the interstrand hairpin?