Question 1

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is _____________________.

Accepted Answer

The answer of The enzyme that catalyzes the formation of...

Question 2

Choose the correct answer from the list below. Not all of the answers will be used.
-The enzyme that joins two DNA molecules is _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 3

A number of tools are critical to gene exploration. Name at least four.

Accepted Answer

Tools include restriction-enzy

Question 4

The biological role of restriction enzymes in bacteria is to:

Accepted Answer

A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  All of the above. 
E)  None of the above. 
A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  All of the above. 
E)  None of the above.

Question 5

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ is the enzyme used to create DNA from RNA.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 6

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________: A collection of DNA sequences representing all of the mRNA expressed in a particular cell type.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 7

What is the basis of the Sanger method?

Accepted Answer

Sanger sequencing is based upon replicat

Exam 41: Recombinant DNA Techniques

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is _____________________.

Choose the correct answer from the list below. Not all of the answers will be used. -The enzyme that joins two DNA molecules is _______________.

A number of tools are critical to gene exploration. Name at least four.

The biological role of restriction enzymes in bacteria is to:

Choose the correct answer from the list below. Not all of the answers will be used. -_______________ is the enzyme used to create DNA from RNA.

Choose the correct answer from the list below. Not all of the answers will be used. -_______________: A collection of DNA sequences representing all of the mRNA expressed in a particular cell type.

What is the basis of the Sanger method?

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

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