Question 1

Briefly outline how a cDNA library is made.

Accepted Answer

Reverse transcriptase is used to convert

Question 2

Briefly outline the steps necessary to create a recombinant DNA molecule.

Accepted Answer

Both the fragment of interest and the ve

Question 3

The technique in which DNA fragments are separated by electrophoresis and identified using a radiolabeled probe is called _____________________.

Accepted Answer

The answer of The technique in which DNA fragments are...

Question 4

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as _____________________.

Accepted Answer

sticky end

Question 5

Choose the correct answer from the list below. Not all of the answers will be used.
-A plasmid is an example of a common _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 6

Plasmids used in recombinant DNA technology typically:

Accepted Answer

A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  All of the above. 
E)  A and B. 
A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  All of the above. 
E)  A and B.

Question 7

_____________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Accepted Answer

Restrictio

Question 8

Design a potential DNA-restriction enzyme site. Show both strands.

Accepted Answer

Any palindromic site

Question 9

How can DNA fragments of various sizes be separated?

Accepted Answer

Electrophoresis is used to separate DNA.

Question 10

Which of the following DNA sequences contains a 4-8 base palindromic site? (Note: Only one strand is shown.)

Accepted Answer

A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC 
A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC

Question 11

A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is:

Accepted Answer

A)  None of the below. 
B)  the Southwestern blot. 
C)  the Western blot. 
D)  the Southern blot. 
E)  the Northern blot. 
A)  None of the below. 
B)  the Southwestern blot. 
C)  the Western blot. 
D)  the Southern blot. 
E)  the Northern blot.

Question 12

The enzyme _____________________ can be used to add nucleotides to the 3` end of DNA.

Accepted Answer

terminal t

Question 13

DNA fragments can be visualized in polyacrylamide-gel electrophoresis by staining with _____________________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light.

Accepted Answer

The answer of DNA fragments can be visualized in polyacrylamide-gel...

Question 14

Reverse transcriptase is normally found in:

Accepted Answer

A)  plants. 
B)  retrovirus. 
C)  mitochondria. 
D)  All of the above.` 
E)  None of the above. 
A)  plants. 
B)  retrovirus. 
C)  mitochondria. 
D)  All of the above.` 
E)  None of the above.

Question 15

For identification of a gene, against which strand of DNA must the probe complement?

Accepted Answer

A)  either strand 
B)  both strands 
C)  only the coding strand 
D)  only the template strand 
E)  None of the above. 
A)  either strand 
B)  both strands 
C)  only the coding strand 
D)  only the template strand 
E)  None of the above.

Question 16

What do Southern, Northern, and Western blots detect, respectively?

Accepted Answer

A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA 
A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA

Question 17

Choose the correct answer from the list below. Not all of the answers will be used.
-DNA sequencing by controlled termination of replication is called the ______________ method.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 18

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ are enzymes that split DNA at specific base-pair sequences.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 19

The probe used to isolate a gene from a genomic library is often:

Accepted Answer

A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  All of the above. 
E)  None of the above. 
A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  All of the above. 
E)  None of the above.

Question 20

Describe the technique called a DNA microarray.

Accepted Answer

Oligonucleotides or cDNAs are affixed to

Exam 41: Recombinant DNA Techniques

Briefly outline how a cDNA library is made.

Briefly outline the steps necessary to create a recombinant DNA molecule.

The technique in which DNA fragments are separated by electrophoresis and identified using a radiolabeled probe is called _____________________.

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as _____________________.

Choose the correct answer from the list below. Not all of the answers will be used. -A plasmid is an example of a common _______________.

Plasmids used in recombinant DNA technology typically:

_____________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Design a potential DNA-restriction enzyme site. Show both strands.

How can DNA fragments of various sizes be separated?

Which of the following DNA sequences contains a 4-8 base palindromic site? (Note: Only one strand is shown.)

A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is:

The enzyme _____________________ can be used to add nucleotides to the 3` end of DNA.

DNA fragments can be visualized in polyacrylamide-gel electrophoresis by staining with _____________________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light.

Reverse transcriptase is normally found in:

For identification of a gene, against which strand of DNA must the probe complement?

What do Southern, Northern, and Western blots detect, respectively?

Choose the correct answer from the list below. Not all of the answers will be used. -DNA sequencing by controlled termination of replication is called the ______________ method.

Choose the correct answer from the list below. Not all of the answers will be used. -_______________ are enzymes that split DNA at specific base-pair sequences.

The probe used to isolate a gene from a genomic library is often:

Describe the technique called a DNA microarray.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

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