Question 1

During postsynapsis strand exchange in homologous recombination of E.coli,ATP is necessary for _______ clearance from the synapsing DNA.

Accepted Answer

RecA

Question 2

All organisms undergo homologous recombination as a way to increase genetic variation and help cope with DNA damage.

Accepted Answer

A) True 
 B)False  True

Question 3

The role of SSB in RecBCD homologous recombination appears to be to unwind the secondary structures found in dsDNA.

Accepted Answer

A) True 
 B)False  True

Question 4

E.coli RecA protein is only necessary for the presynapsis step of homologous recombination.

Accepted Answer

A) True 
 B)False

Question 5

The E.coli genome,on average,contains approximately __________chi sites that can be involved in homologous recombination.

Accepted Answer

The answer of The E.coli genome,on average,contains approximately __________chi sites...

Question 6

One mechanism by which gene conversion can occur in N.crassa during meiosis is _______.

Accepted Answer

A) RecBCD 
B) mismatch repair 
C) double stranded break synthesis 
D) SOS repair 
E) excision repair 
A) RecBCD 
B) mismatch repair 
C) double stranded break synthesis 
D) SOS repair 
E) excision repair

Question 7

________resolvase clips DNA at the consensus sequence,5'-(A/T)$\downarrow$(G/C)-3'during RecBCD recombination repair.

Accepted Answer

The answer of ________resolvase clips DNA at the consensus sequence,5'-(A/T)$\downarrow$(G/C)-3'during...

Question 8

Spo11 creates __________ in DNA during homologous recombination in Saccharomyces cerevisiae.

Accepted Answer

double str

Question 9

During mating between two strains of Neurospora crassa containing different alleles of the same gene one would expect a 1:1 genotypic ratio in progeny spores,however,approximately 0.1% of the time this ratio strays from the predicted.This phenomenon is called_____________ ___________________.

Accepted Answer

The answer of During mating between two strains of Neurospora...

Question 10

Molecular modeling of RuvA and a Holliday junction showed that they form a _________ that favors rapid branch migration.

Accepted Answer

A) square planar conformation 
B) L-shape conformation 
C) clover leaf conformation 
D) D loop conformation 
E) Y-shape conformation 
A) square planar conformation 
B) L-shape conformation 
C) clover leaf conformation 
D) D loop conformation 
E) Y-shape conformation

Question 11

Double stranded break formation in mitotic recombination is followed by 3'$\rightarrow$5' exonuclease digestion of 3' ends at the break to yield 5' overhangs.

Accepted Answer

A) True 
 B)False

Question 12

David Gonda and Charles Radding have provided experimental evidence that the minimum degree of homology necessary for efficient synapsis to occur during homologous recombination is between 30 and 151 bp.

Accepted Answer

A) True 
 B)False

Question 13

RuvA and RuvB are involved in ________ of a Holliday junction during homologous recombination.

Accepted Answer

The answer of RuvA and RuvB are involved in ________...

Question 14

________________ recombination is a cross-over event that joins previously separated DNA segments.

Accepted Answer

The answer of ________________ recombination is a cross-over event that...

Question 15

ATP$\gamma$S is an unhydrolyzable ATP analog that was used along with glutaraldehyde by Parsons and West to trap Holliday junctions for gel mobility shift assays.

Accepted Answer

A) True 
 B)False

Question 16

Both RuvA and RuvB have ATPase activity that drives the unwinding and rewinding of DNA during branch migration.

Accepted Answer

A) True 
 B)False

Question 17

Which of the following techniques was key in demonstrating the presynapsis and synapsis steps of homologous recombination?

Accepted Answer

A) S1 nuclease mapping 
B) exonuclease digestion 
C) DNA footprinting 
D) Southern Blot 
E) electron microscopy 
A) S1 nuclease mapping 
B) exonuclease digestion 
C) DNA footprinting 
D) Southern Blot 
E) electron microscopy

Question 18

Branch migration is not necessary for efficient resolution of Holliday junctions.

Accepted Answer

A) True 
 B)False

Question 19

Mutations in ruvC increase sensitivity to UV light due to defective recombination repair.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following statements is not true concerning homologous recombination?

Accepted Answer

A) It can be intramolecular. 
B) It can be bimolecular. 
C) Only one cross-over event can occur. 
D) It results in genetic variation. 
E) It is a reciprocal event. 
A) It can be intramolecular. 
B) It can be bimolecular. 
C) Only one cross-over event can occur. 
D) It results in genetic variation. 
E) It is a reciprocal event.

Exam 22: Homologous Recombination

During postsynapsis strand exchange in homologous recombination of E.coli,ATP is necessary for _______ clearance from the synapsing DNA.

All organisms undergo homologous recombination as a way to increase genetic variation and help cope with DNA damage.

The role of SSB in RecBCD homologous recombination appears to be to unwind the secondary structures found in dsDNA.

E.coli RecA protein is only necessary for the presynapsis step of homologous recombination.

The E.coli genome,on average,contains approximately __________chi sites that can be involved in homologous recombination.

One mechanism by which gene conversion can occur in N.crassa during meiosis is _______.

________resolvase clips DNA at the consensus sequence,5'-(A/T) ↓\downarrow↓ (G/C)-3'during RecBCD recombination repair.

Spo11 creates __________ in DNA during homologous recombination in Saccharomyces cerevisiae.

During mating between two strains of Neurospora crassa containing different alleles of the same gene one would expect a 1:1 genotypic ratio in progeny spores,however,approximately 0.1% of the time this ratio strays from the predicted.This phenomenon is called_____________ ___________________.

Molecular modeling of RuvA and a Holliday junction showed that they form a _________ that favors rapid branch migration.

Double stranded break formation in mitotic recombination is followed by 3' →\rightarrow→ 5' exonuclease digestion of 3' ends at the break to yield 5' overhangs.

David Gonda and Charles Radding have provided experimental evidence that the minimum degree of homology necessary for efficient synapsis to occur during homologous recombination is between 30 and 151 bp.

RuvA and RuvB are involved in ________ of a Holliday junction during homologous recombination.

________________ recombination is a cross-over event that joins previously separated DNA segments.

ATP γ\gammaγ S is an unhydrolyzable ATP analog that was used along with glutaraldehyde by Parsons and West to trap Holliday junctions for gel mobility shift assays.

Both RuvA and RuvB have ATPase activity that drives the unwinding and rewinding of DNA during branch migration.

Which of the following techniques was key in demonstrating the presynapsis and synapsis steps of homologous recombination?

Branch migration is not necessary for efficient resolution of Holliday junctions.

Mutations in ruvC increase sensitivity to UV light due to defective recombination repair.

Which of the following statements is not true concerning homologous recombination?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

________resolvase clips DNA at the consensus sequence,5'-(A/T) $\downarrow$ (G/C)-3'during RecBCD recombination repair.

During mating between two strains of Neurospora crassa containing different alleles of the same gene one would expect a 1:1 genotypic ratio in progeny spores,however,approximately 0.1% of the time this ratio strays from the predicted.This phenomenon is called_ _______.

Double stranded break formation in mitotic recombination is followed by 3' $\rightarrow$ 5' exonuclease digestion of 3' ends at the break to yield 5' overhangs.

ATP $\gamma$ S is an unhydrolyzable ATP analog that was used along with glutaraldehyde by Parsons and West to trap Holliday junctions for gel mobility shift assays.