Question 1

Immature mRNA splicing can be established by either exon definition or intron definition.

Accepted Answer

A) True 
 B)False  True

Question 2

Alternative splicing patterns can lead to the production of different protein products from the same gene.

Accepted Answer

A) True 
 B)False  True

Question 3

The process by which introns are removed from immature mRNA is called _____________.

Accepted Answer

RNA splicing

Question 4

Which of the following snRNP is mismatched with its function?

Accepted Answer

A) U1: base pairs with 5' splice site of mRNA. 
B) U2: base pairs with the conserved sequence at splicing branch point. 
C) U4: base pairs with 3'splice site of mRNA. 
D) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon. 
E) U6: base pairs with 5' end of the intron. 
A) U1: base pairs with 5' splice site of mRNA. 
B) U2: base pairs with the conserved sequence at splicing branch point. 
C) U4: base pairs with 3'splice site of mRNA. 
D) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon. 
E) U6: base pairs with 5' end of the intron.

Question 5

Experimental evidence demonstrates that mRNA precursors are spliced via a lariat-shaped intermediate.

Accepted Answer

A) True 
 B)False

Question 6

You have isolated a new species of mRNA using an oligo-dT column.You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns.Below is a representation of the electron micrograph results.Which of the following can you conclude from your experiment?

Accepted Answer

A) The gene contains no introns. 
B) The gene contains one intron. 
C) The gene contains two introns. 
D) The gene contains three introns. 
E) The results are inconclusive. 
A) The gene contains no introns. 
B) The gene contains one intron. 
C) The gene contains two introns. 
D) The gene contains three introns. 
E) The results are inconclusive.

Question 7

Joan Steitz was one of the researchers who used __________ studies to demonstrate that U6 snRNP binds to the 5'end of the intron.

Accepted Answer

The answer of Joan Steitz was one of the researchers...

Question 8

Heterogeneous nuclear RNA (hnRNA)is thought to be a precursor of mRNA.

Accepted Answer

A) True 
 B)False

Question 9

The expressed regions of genes are referred to as ________ while the noncoding regions are called_______.

Accepted Answer

The answer of The expressed regions of genes are referred...

Question 10

Which of the following is not true concerning eukaryotic mRNA processing?

Accepted Answer

A) addition of a 3' poly-A tail 
B) addition of a 5' cap 
C) intron splicing 
D) occurs in the nucleus 
E) occurs in the cytoplasm 
A) addition of a 3' poly-A tail 
B) addition of a 5' cap 
C) intron splicing 
D) occurs in the nucleus 
E) occurs in the cytoplasm

Question 11

Which of the following experiments was useful in determining the existence of introns in the adenovirus genome?

Accepted Answer

A) DNA/DNA hybridization 
B) RNA/RNA hybridization 
C) R-looping experiments 
D) RT-PCR 
E) cDNA cloning experiments 
A) DNA/DNA hybridization 
B) RNA/RNA hybridization 
C) R-looping experiments 
D) RT-PCR 
E) cDNA cloning experiments

Question 12

Which of the following splicing factors is required for the correct 3' splice site selection?

Accepted Answer

A) SC35 
B) Slu7 
C) U11 
D) SF2 
E) SRp55 
A) SC35 
B) Slu7 
C) U11 
D) SF2 
E) SRp55

Question 13

Which of the following is the first snRNP to bind during the assembly stage of the spliceosome cycle?

Accepted Answer

A) U1 
B) U2 
C) U4 
D) U5 
E) U6 
A) U1 
B) U2 
C) U4 
D) U5 
E) U6

Question 14

The catalytic center of the spliceosome appears to include

Accepted Answer

A) Mg2+. 
B) U2 and U6 snRNP. 
C) the branch point region of the intron. 
D) Mg2+ and the branch point region of the intron. 
E) Mg2+,U2,and U6 snRNP,and the branch point region of the intron. 
A) Mg2+. 
B) U2 and U6 snRNP. 
C) the branch point region of the intron. 
D) Mg2+ and the branch point region of the intron. 
E) Mg2+,U2,and U6 snRNP,and the branch point region of the intron.

Question 15

"Snurps" are composed of

Accepted Answer

A) RNA. 
B) protein. 
C) DNA. 
D) RNA and protein. 
E) DNA and RNA. 
A) RNA. 
B) protein. 
C) DNA. 
D) RNA and protein. 
E) DNA and RNA.

Question 16

There are a common set of ___________ Sm proteins that are specific to each snRNA.

Accepted Answer

The answer of There are a common set of ___________...

Question 17

Alternative splicing is involved in _________ determination in fruit flys.

Accepted Answer

The answer of Alternative splicing is involved in _________ determination...

Question 18

The binding of which of the following snRNPs to spliceosome requires ATP?

Accepted Answer

A) U1 
B) U2 
C) U4 
D) U5 
E) U6 
A) U1 
B) U2 
C) U4 
D) U5 
E) U6

Question 19

Base pairing between the 5' splice site and U1 snRNA is the only interaction essential for splicing.

Accepted Answer

A) True 
 B)False

Question 20

The first two bases and the last two bases in the splicing signal consensus sequence are

Accepted Answer

A) GT-AG. 
B) GU-AG. 
C) CU-AG. 
D) GU-AC. 
E) GT-TG. 
A) GT-AG. 
B) GU-AG. 
C) CU-AG. 
D) GU-AC. 
E) GT-TG.

Exam 14: Rna Processing I: Splicing

Immature mRNA splicing can be established by either exon definition or intron definition.

Alternative splicing patterns can lead to the production of different protein products from the same gene.

The process by which introns are removed from immature mRNA is called _____________.

Which of the following snRNP is mismatched with its function?

Experimental evidence demonstrates that mRNA precursors are spliced via a lariat-shaped intermediate.

You have isolated a new species of mRNA using an oligo-dT column.You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns.Below is a representation of the electron micrograph results.Which of the following can you conclude from your experiment?

Joan Steitz was one of the researchers who used __________ studies to demonstrate that U6 snRNP binds to the 5'end of the intron.

Heterogeneous nuclear RNA (hnRNA)is thought to be a precursor of mRNA.

The expressed regions of genes are referred to as ________ while the noncoding regions are called_______.

Which of the following is not true concerning eukaryotic mRNA processing?

Which of the following experiments was useful in determining the existence of introns in the adenovirus genome?

Which of the following splicing factors is required for the correct 3' splice site selection?

Which of the following is the first snRNP to bind during the assembly stage of the spliceosome cycle?

The catalytic center of the spliceosome appears to include

"Snurps" are composed of

There are a common set of ___________ Sm proteins that are specific to each snRNA.

Alternative splicing is involved in _________ determination in fruit flys.

The binding of which of the following snRNPs to spliceosome requires ATP?

Base pairing between the 5' splice site and U1 snRNA is the only interaction essential for splicing.

The first two bases and the last two bases in the splicing signal consensus sequence are

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

The expressed regions of genes are referred to as __ while the noncoding regions are called_.