Question 1

The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA polymerase III $\beta$-subunit.

Accepted Answer

A) True 
 B)False  True

Question 2

During lagging strand synthesis,it is not uncommon for the DNA polymerase III holoenzyme to dissociate completely from the DNA template for approximately two seconds.

Accepted Answer

A) True 
 B)False  False

Question 3

DNA polymerase III is a highly ___________ enzyme,meaning once it binds DNA it catalyzes the addition of several hundreds of nucleotides without falling of the DNA.

Accepted Answer

processive

Question 4

Telomerase enzyme activity was first observed in extracts from Tetrahymena micronuclei.

Accepted Answer

A) True 
 B)False

Question 5

All eukaryotes must protect their telomeres from nucleases and double strand break repair enzymes.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following enzymes is responsible for decatenation of Salmonella typhimurium DNA during replication?

Accepted Answer

A) topoisomerase I 
B) topoisomerase II 
C) topoisomerase III 
D) topoisomerase IV 
E) topoisomerase V 
A) topoisomerase I 
B) topoisomerase II 
C) topoisomerase III 
D) topoisomerase IV 
E) topoisomerase V

Question 7

Replication of the SV40 genome is dependent on the viral large T antigen,which has primase activity.

Accepted Answer

A) True 
 B)False

Question 8

In E.coli,DNA replication begins at a unique site,oriC,in the genome.

Accepted Answer

A) True 
 B)False

Question 9

A primosome can be defined as

Accepted Answer

A) the enzyme that synthesizes primers fro M13 phage replication. 
B) a collection of proteins needed for synthesis of primers for replicating DNA. 
C) a collection of RNA primers. 
D) a DNA molecule that is primed and ready for replication. 
E) an area of DNA near the replication fork waiting to be replicated. 
A) the enzyme that synthesizes primers fro M13 phage replication. 
B) a collection of proteins needed for synthesis of primers for replicating DNA. 
C) a collection of RNA primers. 
D) a DNA molecule that is primed and ready for replication. 
E) an area of DNA near the replication fork waiting to be replicated.

Question 10

Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the "sliding clamp"?

Accepted Answer

A) $\alpha$ 
B) $\beta$ 
C) $\gamma$ 
D) $\chi$ 
E) $\theta$ 
A) $\alpha$ 
B) $\beta$ 
C) $\gamma$ 
D) $\chi$ 
E) $\theta$

Question 11

Without telomeres,linear eukaryotic chromosomes would get shorter and shorter with each round of DNA replication.

Accepted Answer

A) True 
 B)False

Question 12

In vivo,the DNA polymerase III holoenzyme can synthesize DNA at a rate of

Accepted Answer

A) 250 nt/sec. 
B) 500 nt/sec. 
C) 750 nt/sec. 
D) 1000nt/sec. 
E) 2000nt/sec. 
A) 250 nt/sec. 
B) 500 nt/sec. 
C) 750 nt/sec. 
D) 1000nt/sec. 
E) 2000nt/sec.

Question 13

The DNA polymerase III holoenzyme $\gamma$-complex can act as a clamp loader and unloader.

Accepted Answer

A) True 
 B)False

Question 14

The ends of eukaryotic chromosomes are composed of short GC-rich repeat sequences called ____________.

Accepted Answer

The answer of The ends of eukaryotic chromosomes are composed...

Question 15

The DNA polymerase III holoenzyme contains two core enzymes,one that is responsible for ___________ strand synthesis and the other responsible for ____________strand synthesis.

Accepted Answer

The answer of The DNA polymerase III holoenzyme contains two...

Question 16

Telomerase is a unique enzyme in that it is composed of only RNA.

Accepted Answer

A) True 
 B)False

Question 17

Bacterial chromosomes are the only chromosomes known to require decatenation during replication.

Accepted Answer

A) True 
 B)False

Question 18

After DNA replication,bacterial genomes must undergo decatenation before the cell can divide.

Accepted Answer

A) True 
 B)False

Question 19

The DNA polymerase III core plus the ____________ is needed to replicate DNA processively.$\beta$

Accepted Answer

The answer of The DNA polymerase III core plus the...

Question 20

The chromosomal origins of replication vary greatly among bacteria,so much so,that it is hard to propose an origin of replication consensus sequence.

Accepted Answer

A) True 
 B)False

Exam 21: Dna Replication II: Detailed Mechanism

The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA polymerase III β\betaβ -subunit.

During lagging strand synthesis,it is not uncommon for the DNA polymerase III holoenzyme to dissociate completely from the DNA template for approximately two seconds.

DNA polymerase III is a highly ___________ enzyme,meaning once it binds DNA it catalyzes the addition of several hundreds of nucleotides without falling of the DNA.

Telomerase enzyme activity was first observed in extracts from Tetrahymena micronuclei.

All eukaryotes must protect their telomeres from nucleases and double strand break repair enzymes.

Which of the following enzymes is responsible for decatenation of Salmonella typhimurium DNA during replication?

Replication of the SV40 genome is dependent on the viral large T antigen,which has primase activity.

In E.coli,DNA replication begins at a unique site,oriC,in the genome.

A primosome can be defined as

Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the "sliding clamp"?

Without telomeres,linear eukaryotic chromosomes would get shorter and shorter with each round of DNA replication.

In vivo,the DNA polymerase III holoenzyme can synthesize DNA at a rate of

The DNA polymerase III holoenzyme γ\gammaγ -complex can act as a clamp loader and unloader.

The ends of eukaryotic chromosomes are composed of short GC-rich repeat sequences called ____________.

The DNA polymerase III holoenzyme contains two core enzymes,one that is responsible for ___________ strand synthesis and the other responsible for ____________strand synthesis.

Telomerase is a unique enzyme in that it is composed of only RNA.

Bacterial chromosomes are the only chromosomes known to require decatenation during replication.

After DNA replication,bacterial genomes must undergo decatenation before the cell can divide.

The DNA polymerase III core plus the ____________ is needed to replicate DNA processively. β\betaβ

The chromosomal origins of replication vary greatly among bacteria,so much so,that it is hard to propose an origin of replication consensus sequence.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA polymerase III $\beta$ -subunit.

The DNA polymerase III holoenzyme $\gamma$ -complex can act as a clamp loader and unloader.

The DNA polymerase III holoenzyme contains two core enzymes,one that is responsible for _ strand synthesis and the other responsible for __strand synthesis.

The DNA polymerase III core plus the ____________ is needed to replicate DNA processively. $\beta$