Exam 25: Genomics II: Functional Genomics,proteomics and Bioinformatics

Whole chromosome transcriptional mapping revealed that there are more poly(A)⁺ RNA transcripts than poly(A)^- RNA transcripts in human cells.

Computational biology techniques allowed scientists to discover highly conserved sequence motifs in the promoter regions and ___________ of four mammalian species.

The NCBI website contains a vast store of biological information.If you have a sequence that you would like to compare to other known sequences,what is the name of the NCBI database that would allow such a search? ________.

High density whole chromosome transcriptional mapping studies have shown that the majority of sequences in cytoplasmic polyadenylated RNAs derive from non-____________ regions of ten human chromosomes.

Mass spectrometry is not a quantitative method,so it has been difficult to use it to analyze the expression levels of proteins.

Tissue-specific expression profiling can be done by examining the spectrum of mRNAs whose levels are decreased by an exogenous _________ and comparing that to the spectrum of expression of genes at the mRNA level in various tissues.

A _____________ is the sum total of all the RNA transcripts in an organism at one time.

ChIP-chip analysis can be used to identify DNA-binding sites for activators and other proteins.

SNPs or single nucleotide polymorphisms may help physicians design patient specific drug treatment programs.

Tag sequencing or ChIPSeq,in which the chromatin pieces precipitated by ChIP are repeatedly sequenced can be used to identify transcription-factor binding sites.

The level of transcription of a gene gives an exact idea of the real level of the expression of that gene.

Which of the following processes allows scientists to identify the 5'-ends of transcripts?

In one kind of mutation analysis called,__________ __________,mutants are created by replacing genes one at a time with antibiotic resistance genes flanked by oligomers that serve as a barcode to identify each mutant.

The sum total of all proteins produced by an organism is called its____________.

The best tool available for the separation of many proteins at once is called ________.

Which of the following processes allows scientists to determine which genes are expressed in a given tissue and the extent of that expression?

Which of the following is not a finding from analysis of the complete DNA sequence of human chromosome 22?

Which of the following processes allows scientists to 'knock-down' genes?

Yeast two-hybrid is a traditional technique used to probe protein-DNA interactions.

One place to find useful databases for bioinformatics is the _________ website.