Question 1

A ________________ map is used to indicate the positions on genes on a chromosome.

Accepted Answer

physical

Question 2

Which of the following is used to study the interaction of proteins with DNA?

Accepted Answer

A) S1 nuclease protection 
B) DNase-1 footprinting 
C) DNA fingerprinting 
D) southern analysis 
E) northern blotting 
A) S1 nuclease protection 
B) DNase-1 footprinting 
C) DNA fingerprinting 
D) southern analysis 
E) northern blotting B

Question 3

A DNA fragment was treated with EcoRI restriction enzyme.The restriction pattern is shown below.How many fragments should be visible on a Southern blot if radioactively labeled probe "1" was used?

Accepted Answer

A) 0 
B) 1 
C) 2 
D) 3 
E) 4   
A) 0 
B) 1 
C) 2 
D) 3 
E) 4   D

Question 4

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes.EcoRI cuts at position 100.HindIII cuts at positions 500 and 2000.Which of the following represents the fragment that would be observed if the restricted DNA were analzyed using agarose gel electrophoresis?

Accepted Answer

A) 400,100,1000,1500 
B) 400,500,1500 
C) 400,100,1600 
D) 500,100,500,900 
E) 500,1000,600 
A) 400,100,1000,1500 
B) 400,500,1500 
C) 400,100,1600 
D) 500,100,500,900 
E) 500,1000,600

Question 5

A set of six probes was used to compare a DNA sample taken from a crime scene to the DNA of an arrested suspect.Each probe can detect one in every one hundred persons.Results showed that all six probes detected identical patterns in both DNA specimens.What is the chance for a match using all six probes?

Accepted Answer

A) 1 in 106 
B) 1 in 1010 
C) 1 in 1011 
D) 1 in 1012 
E) 1 in 108 
A) 1 in 106 
B) 1 in 1010 
C) 1 in 1011 
D) 1 in 1012 
E) 1 in 108

Question 6

Which of the following is not used in a Sanger chain termination reaction?

Accepted Answer

A) ddATP 
B) ddUTP 
C) ddCTP 
D) ddGTP 
E) ddTTP 
A) ddATP 
B) ddUTP 
C) ddCTP 
D) ddGTP 
E) ddTTP

Question 7

____________ is a method used to detect DNA-protein interactions.

Accepted Answer

The answer of ____________ is a method used to detect...

Question 8

Which of the following is not true about run-on transcription assay?

Accepted Answer

A) Isolated nuclei are used in the assay. 
B) Cytoplasmic RNA is included in the assay. 
C) The reaction is done in the presence of labeled nucleotide. 
D) The assay can reveal the transcription rates of genes 
E) Genes being transcribed can be identified. 
A) Isolated nuclei are used in the assay. 
B) Cytoplasmic RNA is included in the assay. 
C) The reaction is done in the presence of labeled nucleotide. 
D) The assay can reveal the transcription rates of genes 
E) Genes being transcribed can be identified.

Question 9

During SDS PAGE,which of the following does not directly affect the migration of the polypeptides through the gel?

Accepted Answer

A) the size of the polypeptide 
B) the strength of the electric field 
C) the molecular mass of the protein 
D) the native charge on the polypeptides 
E) the SDS 
A) the size of the polypeptide 
B) the strength of the electric field 
C) the molecular mass of the protein 
D) the native charge on the polypeptides 
E) the SDS

Question 10

Site-directed mutagenesis is used for which of the following purposes?

Accepted Answer

A) to insert fragments of more than 100 pairs into a specific DNA site 
B) to induced site-specific recombination 
C) to make changes to specific bases in DNA 
D) to remove large segments of a DNA sequence 
E) Both to induced site-specific recombination and to make changes to specific bases in DNA are correct. 
A) to insert fragments of more than 100 pairs into a specific DNA site 
B) to induced site-specific recombination 
C) to make changes to specific bases in DNA 
D) to remove large segments of a DNA sequence 
E) Both to induced site-specific recombination and to make changes to specific bases in DNA are correct.

Question 11

Which of the following isotopes would be the most appropriate for the end-labeling of a DNA strand with a radioactive phosphate?

Accepted Answer

A) $\alpha$-32P 
B) 35S 
C) $\beta$ -32P 
D) $\gamma$ -32P 
E) 14C 
A) $\alpha$-32P 
B) 35S 
C) $\beta$ -32P 
D) $\gamma$ -32P 
E) 14C

Question 12

Which of the following can be used to establish relatedness between individuals?

Accepted Answer

A) DNA fingerprinting 
B) DNA typing 
C) RFLP analysis 
D) DNAse footprinting 
E) DNA fingerprinting,DNA typing,and RFLP analysis are correct. 
A) DNA fingerprinting 
B) DNA typing 
C) RFLP analysis 
D) DNAse footprinting 
E) DNA fingerprinting,DNA typing,and RFLP analysis are correct.

Question 13

Which of the following is a substrate for $\beta$-galactosidase?

Accepted Answer

A) lacZ 
B) chloramphenicol 
C) X-gal 
D) acetyl-CoA 
E) both lacZ and acetyl-CoA 
A) lacZ 
B) chloramphenicol 
C) X-gal 
D) acetyl-CoA 
E) both lacZ and acetyl-CoA

Question 14

__________ is a means of detecting radioactive compounds with photographic emulsion.

Accepted Answer

The answer of __________ is a means of detecting radioactive...

Question 15

A major function of reporter gene assays is to

Accepted Answer

A) measure mutation rates of a gene. 
B) measure transcriptional levels. 
C) measure replication. 
D) monitor luciferase activity. 
E) monitor CAT activity. 
A) measure mutation rates of a gene. 
B) measure transcriptional levels. 
C) measure replication. 
D) monitor luciferase activity. 
E) monitor CAT activity.

Question 16

A circular 3000 bp UC18 plasmid DNA sample was treated with BamHI restriction enzyme.The plasmid has restriction sites at position 100 and position 2000.Which of the following choices represents the size fragments that would be observed on an agarose gel?

Accepted Answer

A) 2900,100 
B) 1900,100,1000 
C) 1900,1100 
D) 2900,50,50 
E) 1900,50,50,1000 
A) 2900,100 
B) 1900,100,1000 
C) 1900,1100 
D) 2900,50,50 
E) 1900,50,50,1000

Question 17

It is suspected that a novel histone protein only interacts with the promoter region of $\beta$-globin genes.Which of the following techniques should be used to confirm this suspicion?

Accepted Answer

A) EMSA 
B) DNase footprinting 
C) filter binding 
D) gel mobility shift assay 
E) all of the choices are correct. 
A) EMSA 
B) DNase footprinting 
C) filter binding 
D) gel mobility shift assay 
E) all of the choices are correct.

Question 18

A new insulin gene was isolated and a study was planned to look at the function of the promoter region.Which of the following would be the best choice for this study?

Accepted Answer

A) southern analysis 
B) immunoblotting 
C) reporter gene assay 
D) PCR 
E) immunoprecipitation 
A) southern analysis 
B) immunoblotting 
C) reporter gene assay 
D) PCR 
E) immunoprecipitation

Question 19

In molecular biology all of the following methods can detect small traces of radioactivity except

Accepted Answer

A) liquid scintillation counting. 
B) autoradiography. 
C) x-ray film. 
D) phosphorimaging. 
E) spectrophotometry. 
A) liquid scintillation counting. 
B) autoradiography. 
C) x-ray film. 
D) phosphorimaging. 
E) spectrophotometry.

Question 20

The best method for separating large DNA fragments greater that 1Mb is by electrophoresis in 1% agarose gels.

Accepted Answer

A) True 
 B)False

Exam 5: Molecular Tools for Studying Genes and Gene Activity

A ________________ map is used to indicate the positions on genes on a chromosome.

Which of the following is used to study the interaction of proteins with DNA?

A DNA fragment was treated with EcoRI restriction enzyme.The restriction pattern is shown below.How many fragments should be visible on a Southern blot if radioactively labeled probe "1" was used?

A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes.EcoRI cuts at position 100.HindIII cuts at positions 500 and 2000.Which of the following represents the fragment that would be observed if the restricted DNA were analzyed using agarose gel electrophoresis?

Which of the following is not used in a Sanger chain termination reaction?

____________ is a method used to detect DNA-protein interactions.

Which of the following is not true about run-on transcription assay?

During SDS PAGE,which of the following does not directly affect the migration of the polypeptides through the gel?

Site-directed mutagenesis is used for which of the following purposes?

Which of the following isotopes would be the most appropriate for the end-labeling of a DNA strand with a radioactive phosphate?

Which of the following can be used to establish relatedness between individuals?

Which of the following is a substrate for β\betaβ -galactosidase?

__________ is a means of detecting radioactive compounds with photographic emulsion.

A major function of reporter gene assays is to

A circular 3000 bp UC18 plasmid DNA sample was treated with BamHI restriction enzyme.The plasmid has restriction sites at position 100 and position 2000.Which of the following choices represents the size fragments that would be observed on an agarose gel?

It is suspected that a novel histone protein only interacts with the promoter region of β\betaβ -globin genes.Which of the following techniques should be used to confirm this suspicion?

A new insulin gene was isolated and a study was planned to look at the function of the promoter region.Which of the following would be the best choice for this study?

In molecular biology all of the following methods can detect small traces of radioactivity except

The best method for separating large DNA fragments greater that 1Mb is by electrophoresis in 1% agarose gels.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

Which of the following is a substrate for $\beta$ -galactosidase?

It is suspected that a novel histone protein only interacts with the promoter region of $\beta$ -globin genes.Which of the following techniques should be used to confirm this suspicion?