Question 1

UV radiation can trigger the lytic cycle in $\lambda$ phage.

Accepted Answer

A) True 
 B)False  True

Question 2

O,P,S,and R genes are part of the lambda phage genome.A new clone of lambda was constructed that lacked the O and P region.Which of the following would be observed if this new clone were used to infect bacteria?

Accepted Answer

A) The clone would not enter the cell. 
B) The clone would be destroyed by the bacterial host. 
C) Replication of this would be impaired in the lytic phase. 
D) A rapid burst of replication would occur. 
E) The clone would not enter the cell and replication of this would be impaired in the lytic phase are correct. 
A) The clone would not enter the cell. 
B) The clone would be destroyed by the bacterial host. 
C) Replication of this would be impaired in the lytic phase. 
D) A rapid burst of replication would occur. 
E) The clone would not enter the cell and replication of this would be impaired in the lytic phase are correct. C

Question 3

Which of the following is true about the interaction with the $\lambda$ repressor and RNA polymerase at PRM?

Accepted Answer

A) Interaction between repressor and polymerase is essential for activation of transcription. 
B) Interaction involves region 4 of $\sigma$-factor. 
C) Interaction can be disrupted by the binding of CIII. 
D) Interaction between repressor and polymerase is essential for activation of transcription and interaction involves region 4 of $\sigma$-factor are correct. 
E) Interaction between repressor and polymerase is essential for activation of transcription and interaction can be disrupted by the binding of CIII are correct. 
A) Interaction between repressor and polymerase is essential for activation of transcription. 
B) Interaction involves region 4 of $\sigma$-factor. 
C) Interaction can be disrupted by the binding of CIII. 
D) Interaction between repressor and polymerase is essential for activation of transcription and interaction involves region 4 of $\sigma$-factor are correct. 
E) Interaction between repressor and polymerase is essential for activation of transcription and interaction can be disrupted by the binding of CIII are correct. D

Question 4

Which of the following is a possible mechanism for the action of NusA in termination?

Accepted Answer

A) It binds to the RNA polymerase causing it to stall. 
B) It stimulates termination at intrinsic terminator by facilitating hairpin loop formation. 
C) It binds to NusB to promote detachment of the RNA polymerase for the DNA template. 
D) It interacts with S10 to promote dissociation of the RNA polymerase. 
E) It binds to the RNA polymerase causing it to stall and it stimulates termination at intrinsic terminator by facilitating hairpin loop formation. 
A) It binds to the RNA polymerase causing it to stall. 
B) It stimulates termination at intrinsic terminator by facilitating hairpin loop formation. 
C) It binds to NusB to promote detachment of the RNA polymerase for the DNA template. 
D) It interacts with S10 to promote dissociation of the RNA polymerase. 
E) It binds to the RNA polymerase causing it to stall and it stimulates termination at intrinsic terminator by facilitating hairpin loop formation.

Question 5

Heat shock genes encode proteins that lead to the rapid death of cells when they are exposed to heat.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following is a target of the Cro protein?

Accepted Answer

A) OR2 
B) OR3 
C) OL3 
D) OR2 and OR3 
E) OR3 and OL3 
A) OR2 
B) OR3 
C) OL3 
D) OR2 and OR3 
E) OR3 and OL3

Question 7

An experiment is planned to look at the transcription start site for the spoIID gene during sporulation.Which of the following techniques would yield the most information in this experiment?

Accepted Answer

A) southern analysis 
B) northern anlaysis 
C) S1 mapping 
D) run-on assays 
E) DNAse footprinting 
A) southern analysis 
B) northern anlaysis 
C) S1 mapping 
D) run-on assays 
E) DNAse footprinting

Question 8

Hairpin formation is necessary for ____________________.

Accepted Answer

The answer of Hairpin formation is necessary for ____________________....

Question 9

Which of the following is an incorrect statement regarding antitermination?

Accepted Answer

A) N is involved in termination of the later genes during l phage infection. 
B) Antitermination in the l late region requires Q. 
C) Five proteins collaborate in antitermination in the l immediate early gene terminators. 
D) Both N is involved in termination of the later genes during l phage infection and Antitermination in the l late region requires Q are false. 
E) N is involved in termination of the later genes during l phage infection,Antitermination in the l late region requires Q,and Five proteins collaborate in antitermination in the l immediate early gene terminators are false. 
A) N is involved in termination of the later genes during l phage infection. 
B) Antitermination in the l late region requires Q. 
C) Five proteins collaborate in antitermination in the l immediate early gene terminators. 
D) Both N is involved in termination of the later genes during l phage infection and Antitermination in the l late region requires Q are false. 
E) N is involved in termination of the later genes during l phage infection,Antitermination in the l late region requires Q,and Five proteins collaborate in antitermination in the l immediate early gene terminators are false.

Question 10

When a $\lambda$ lysogen suffers DNA damage,it induces the ____________ response.

Accepted Answer

The answer of When a $\lambda$ lysogen suffers DNA damage,it...

Question 11

T7 phage encodes a new RNA polymerase for use in the later stages of its bacterial host.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following operators is a target of cI gene production?

Accepted Answer

A) OR1 
B) OR2 
C) OL1 
D) OL2 
E) all of the choices are correct. 
A) OR1 
B) OR2 
C) OL1 
D) OL2 
E) all of the choices are correct.

Question 13

PRE is the promoter used to establish _____________ in $\lambda$ page.

Accepted Answer

The answer of PRE is the promoter used to establish...

Question 14

Which of the following is involved in maintaining lysogeny in $\lambda$ phage?

Accepted Answer

A) PRE 
B) PRM 
C) PRL 
D) PRO 
E) all of the choices are correct. 
A) PRE 
B) PRM 
C) PRL 
D) PRO 
E) all of the choices are correct.

Question 15

Which of the following statements is true about the cro gene?

Accepted Answer

A) It must be stimulated during lysogeny. 
B) Its product promotes repressor activity. 
C) cro repression is important to lysogeny. 
D) It is adjacent to the cIII gene. 
E) It must be stimulated during lysogeny and its product promotes repressor activity are correct. 
A) It must be stimulated during lysogeny. 
B) Its product promotes repressor activity. 
C) cro repression is important to lysogeny. 
D) It is adjacent to the cIII gene. 
E) It must be stimulated during lysogeny and its product promotes repressor activity are correct.

Question 16

___________ ____________ bind to unfolded proteins and help them to fold properly.

Accepted Answer

Molecular

Question 17

When phage infects a bacterium,it usually subverts the host's transcription machinery to use as its own.

Accepted Answer

A) True 
 B)False

Question 18

Predict the outcome of the removal of the N gene from the DNA sequence of the $\lambda$ phage:

Accepted Answer

A) lysogenic cycle will be induced. 
B) entry into the bacterial host will be blocked. 
C) antitermination will be affected. 
D) transcription from the left promoter will be interrupted. 
E) antitermination will be affected and transcription from the left promoter will be interrupted are correct. 
A) lysogenic cycle will be induced. 
B) entry into the bacterial host will be blocked. 
C) antitermination will be affected. 
D) transcription from the left promoter will be interrupted. 
E) antitermination will be affected and transcription from the left promoter will be interrupted are correct.

Question 19

_________________ suppression occurs when a mutation in one gene suppresses the mutation in another gene.

Accepted Answer

The answer of _________________ suppression occurs when a mutation in...

Question 20

Which of the following proteins is not involved the lytic phase of $\lambda$ infection of bacteria?

Accepted Answer

A) CIII 
B) S10 
C) NusA 
D) NusG 
E) NusB 
A) CIII 
B) S10 
C) NusA 
D) NusG 
E) NusB

Exam 8: Major Shifts in Bacterial Transcription

UV radiation can trigger the lytic cycle in λ\lambdaλ phage.

O,P,S,and R genes are part of the lambda phage genome.A new clone of lambda was constructed that lacked the O and P region.Which of the following would be observed if this new clone were used to infect bacteria?

Which of the following is true about the interaction with the λ\lambdaλ repressor and RNA polymerase at PRM?

Which of the following is a possible mechanism for the action of NusA in termination?

Heat shock genes encode proteins that lead to the rapid death of cells when they are exposed to heat.

Which of the following is a target of the Cro protein?

An experiment is planned to look at the transcription start site for the spoIID gene during sporulation.Which of the following techniques would yield the most information in this experiment?

Hairpin formation is necessary for ____________________.

Which of the following is an incorrect statement regarding antitermination?

When a λ\lambdaλ lysogen suffers DNA damage,it induces the ____________ response.

T7 phage encodes a new RNA polymerase for use in the later stages of its bacterial host.

Which of the following operators is a target of cI gene production?

PRE is the promoter used to establish _____________ in λ\lambdaλ page.

Which of the following is involved in maintaining lysogeny in λ\lambdaλ phage?

Which of the following statements is true about the cro gene?

___________ ____________ bind to unfolded proteins and help them to fold properly.

When phage infects a bacterium,it usually subverts the host's transcription machinery to use as its own.

Predict the outcome of the removal of the N gene from the DNA sequence of the λ\lambdaλ phage:

_________________ suppression occurs when a mutation in one gene suppresses the mutation in another gene.

Which of the following proteins is not involved the lytic phase of λ\lambdaλ infection of bacteria?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

UV radiation can trigger the lytic cycle in $\lambda$ phage.

Which of the following is true about the interaction with the $\lambda$ repressor and RNA polymerase at P_RM?

When a $\lambda$ lysogen suffers DNA damage,it induces the ____________ response.

P_RE is the promoter used to establish _____________ in $\lambda$ page.

Which of the following is involved in maintaining lysogeny in $\lambda$ phage?

_ __ bind to unfolded proteins and help them to fold properly.

Predict the outcome of the removal of the N gene from the DNA sequence of the $\lambda$ phage:

Which of the following proteins is not involved the lytic phase of $\lambda$ infection of bacteria?