Exam 4: Molecular Cloning Methods

A fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends.

Which of the following enzymes cannot catalyze the formation of a phosphodiester bond?

A bacterial sample was contaminated with an unknown preparation of vector DNA.In order to identify the vector,the bacteria was streaked on a plate and incubated overnight.Examination of the plate revealed at least 120 clear plaques.Which of the following is a plausible conclusion?

How many enzymes should be used to cut the plasmid DNA and the insert DNA if there is a desire to ligate the insert within the vector in a specific orientation?

A fusion protein can easily be isolated by ____________________ chromatography.

In a replacement vector,a portion of the DNA is removed to accommodate the fragment to be inserted.

One approach to prevent religation of the ends of a restricted plasmid DNA is to

In the construction of an expression vector,which of the following would you include in order to stimulate a high level of RNA synthesis?

A group of identical cells is called a __________.

A recombinant plasmid was constructed with several restriction enzyme sites and an ampicillin resistance marker.This recombinant was used to transform a host bacteria that also has the ampicillin resistant gene.Which of the following statements is true regarding this experiment?

Polynucleotide probes can be used to screen a genomic library for specific genomic sequences.

The "Flavr Savr" tomato is generated by

HindIII is so named because it was isolated from Haemophilus influenzae bacterium.

The complementary sequence of one of the following is a palindrome.Which one?

Which of the following is true regarding alpha complementation?

Which of the following is ideal for screening a protein expression library?

Restriction enzymes were discovered by

A molecular biologist is able to select recombinant colonies from a bacterial culture plate based on the color of the colonies.Which of the following is a correct statement?

Bacteria use the restriction-modification system to synthesize new DNA molecules.

Select the component that is most critical to the function of a plasmid.