Question 1

Which of the following is not a characteristic of prokaryotic single stranded DNA binding proteins?

Accepted Answer

A) They are not essential for cell replication. 
B) They stimulate homologous DNA polymerase. 
C) They aid helicase action. 
D) They keep DNA strands from annealing. 
E) They bind more tightly to ssDNA than to dsDNA. 
A) They are not essential for cell replication. 
B) They stimulate homologous DNA polymerase. 
C) They aid helicase action. 
D) They keep DNA strands from annealing. 
E) They bind more tightly to ssDNA than to dsDNA. A

Question 2

The __________ strand in DNA replication is synthesized discontinuously.

Accepted Answer

lagging

Question 3

The DNA polymerase involved in error-free by-pass that inserts dAMPs across from pyrimidine dimers is

Accepted Answer

A) DNA Pol I. 
B) DNA Pol II. 
C) DNA Pol III. 
D) DNA Pol $ \eta $. 
E) DNA Pol  . 
A) DNA Pol I. 
B) DNA Pol II. 
C) DNA Pol III. 
D) DNA Pol $ \eta $. 
E) DNA Pol  . D

Question 4

In most organisms the direction of DNA replication is ______________.

Accepted Answer

The answer of In most organisms the direction of DNA...

Question 5

E.coli Base Excision repair starts with the enzyme ___________ clipping out the damaged base and leaving an apurinic or apyrimidinic site.

Accepted Answer

The answer of E.coli Base Excision repair starts with the...

Question 6

Chromatin remodeling is required for both homologous recombination and nonhomologous end-joining.

Accepted Answer

A) True 
 B)False

Question 7

In error-free bypass,DNA polymerase $ \eta $ inserts dAMPs across from pyrimidine dimers,thereby correcting all DNA damage caused by UV radiation.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following statements is not true concerning DNA replication in prokaryotes?

Accepted Answer

A) DNA replication is semiconservative. 
B) DNA replication is semidiscontinuous. 
C) DNA replication is RNA dependent. 
D) DNA replication requires only one enzyme. 
E) There is one origin of replication. 
A) DNA replication is semiconservative. 
B) DNA replication is semidiscontinuous. 
C) DNA replication is RNA dependent. 
D) DNA replication requires only one enzyme. 
E) There is one origin of replication.

Question 9

Enzymes that introduce transient single stranded or double stranded breaks in DNA are called______________.

Accepted Answer

The answer of Enzymes that introduce transient single stranded or...

Question 10

The _________ fragment of DNA Pol I is commonly used in vitro to perform DNA end-filling experiments.

Accepted Answer

The answer of The _________ fragment of DNA Pol I...

Question 11

DNA polymerase synthesizes DNA in the ___ to ____ direction.'

Accepted Answer

The answer of DNA polymerase synthesizes DNA in the ___...

Question 12

DNA photolyase in human cells is involved in breaking pyrimidine dimers formed by UV radiation.

Accepted Answer

A) True 
 B)False

Question 13

_____________ refers to the fact that once DNA polymerase holoenzyme starts replicating DNA it remains bound to the DNA template and catalyzes the addition of several nucleotides to the growing strand.

Accepted Answer

The answer of _____________ refers to the fact that once...

Question 14

Which of the following mammalian cell DNA polymerases is not correctly matched with its function?

Accepted Answer

A) DNA Pol$\alpha$-priming DNA synthesis 
B) DNA Pol $\beta$-DNA repair 
C) DNA Pol $\gamma$-elongation of both strands 
D) DNA Pol   $\delta$ -ligation of DNA strands 
E) DNA Pol $\varepsilon$-DNA repair 
A) DNA Pol$\alpha$-priming DNA synthesis 
B) DNA Pol $\beta$-DNA repair 
C) DNA Pol $\gamma$-elongation of both strands 
D) DNA Pol   $\delta$ -ligation of DNA strands 
E) DNA Pol $\varepsilon$-DNA repair

Question 15

The most common type of DNA damage repair is

Accepted Answer

A) excision repair. 
B) photoreactivation. 
C) suicide enzyme repair. 
D) DNA photolyase. 
E) proofreading repair. 
A) excision repair. 
B) photoreactivation. 
C) suicide enzyme repair. 
D) DNA photolyase. 
E) proofreading repair.

Question 16

In transcription,coupled nucleotide excision repair RNA polymerase plays a role in detecting and melting damaged DNA.

Accepted Answer

A) True 
 B)False

Question 17

In semiconservative replication of DNA the two parental strands stay together and the two new daughter strands are produced.

Accepted Answer

A) True 
 B)False

Question 18

E.coli dnaB temperature sensitive mutants cannot undergo DNA replication at 40oC due to the loss of helicase activity.

Accepted Answer

A) True 
 B)False

Question 19

Another term for the Nucleotide Excision Repair enzyme system is_____________.

Accepted Answer

The answer of Another term for the Nucleotide Excision Repair...

Question 20

The process by which electrophiles,such as ethylmethane sulfonate,add carbon containing groups to DNA is called____________.

Accepted Answer

The answer of The process by which electrophiles,such as ethylmethane...

Exam 20: Dna Replication,damage and Repair

Which of the following is not a characteristic of prokaryotic single stranded DNA binding proteins?

The __________ strand in DNA replication is synthesized discontinuously.

The DNA polymerase involved in error-free by-pass that inserts dAMPs across from pyrimidine dimers is

In most organisms the direction of DNA replication is ______________.

E.coli Base Excision repair starts with the enzyme ___________ clipping out the damaged base and leaving an apurinic or apyrimidinic site.

Chromatin remodeling is required for both homologous recombination and nonhomologous end-joining.

In error-free bypass,DNA polymerase η \eta η inserts dAMPs across from pyrimidine dimers,thereby correcting all DNA damage caused by UV radiation.

Which of the following statements is not true concerning DNA replication in prokaryotes?

Enzymes that introduce transient single stranded or double stranded breaks in DNA are called______________.

The _________ fragment of DNA Pol I is commonly used in vitro to perform DNA end-filling experiments.

DNA polymerase synthesizes DNA in the ___ to ____ direction.'

DNA photolyase in human cells is involved in breaking pyrimidine dimers formed by UV radiation.

_____________ refers to the fact that once DNA polymerase holoenzyme starts replicating DNA it remains bound to the DNA template and catalyzes the addition of several nucleotides to the growing strand.

Which of the following mammalian cell DNA polymerases is not correctly matched with its function?

The most common type of DNA damage repair is

In transcription,coupled nucleotide excision repair RNA polymerase plays a role in detecting and melting damaged DNA.

In semiconservative replication of DNA the two parental strands stay together and the two new daughter strands are produced.

E.coli dnaB temperature sensitive mutants cannot undergo DNA replication at 40oC due to the loss of helicase activity.

Another term for the Nucleotide Excision Repair enzyme system is_____________.

The process by which electrophiles,such as ethylmethane sulfonate,add carbon containing groups to DNA is called____________.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

In error-free bypass,DNA polymerase $\eta$ inserts dAMPs across from pyrimidine dimers,thereby correcting all DNA damage caused by UV radiation.

DNA polymerase synthesizes DNA in the _ to __ direction.'

E.coli dnaB temperature sensitive mutants cannot undergo DNA replication at 40^oC due to the loss of helicase activity.