Exam 6: The Mechanism of Transcription in Bacteria

Which of the following precursors would be most appropriate to use in an experiment of the first 10 nucleotides in the initiation phase of transcription?

______________ can be used to block initiation of RNA synthesis.

A closed promoter complex is one in which the holoenzyme is loosely bound and the DNA remains in double-stranded form.

The core RNA polymerase must bind tightly to the promoter in order to initiate transcription.

An experiment was designed to obtain nonspecific transcription from both strands of a DNA molecule.Which of the following strategies would be most effective in achieving this?

The alpha subunit recognizes the UP promoter element.

Which of the following is incorrect regarding rho-dependent terminators?

Which of the following are characteristic of intrinsic terminator elements?

Which of the following is not present in the core RNA polymerase?

The sigma subunit in RNA polymerase is necessary for the transcription of intermediate genes and RNA elongation.

As transcription proceeds,the strain introduced in DNA is relaxed by DNA topoisomerases.

Which of the following statement is false regarding the sigma-factor?

Which of the following does not have a DNA-binding domain?

The catalytic centers of some polymerase cores contain magnesium ions.

Place the following in the order that they occur during transcription initiation: 1 - formation of open complex; 2 - formation of closed complex; 3 promoter clearance; 4 - synthesis of about 10 nucleotides

The FRET technique allows the study of the movement of two molecules relative to each other.

Which of the following would most likely occur if an in vitro transcription assay is performed using a cell extract containing an alpha subunit without the C-terminal domain?

The DNA binding sites for RNA polymerases are called ______________.

Which of the following techniques would be most useful in testing the ability of the core RNA polymerase to bind to the promoter?

Which of the following techniques is suitable for the study of DNA:RNA hybrid formation during transcription?