Exam 7: Operons: Fine Control of Bacterial Transcription

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One hypothesis explaining the mechanism of repression of the lac operon states that the repressor binds to the operator and blocks access by the polymerase to the adjacent promoter.

Free

(True/False)

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(39)

Question 1

Correct Answer:

Verified

True

The ara operon is controlled by the _____________ protein.

Free

(Short Answer)

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Question 2

Correct Answer:

Verified

AraC

Which of the following is true about the action of CAP at the lac promoter?

Free

(Multiple Choice)

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(34)

Question 3

Correct Answer:

Verified

Which of the following is true about the regulation of the trp operon?

(Multiple Choice)

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Question 4

If an operon is under negative control it means that the operon is never operational.

(True/False)

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Question 5

Arabinose can derepress the ara operon by causing AraC to loosen its attachment to araO₂ and to bind to araI₂ instead.

(True/False)

4.9/5

(36)

Question 6

Which of the following is most likely to occur if the level of glucose is low in a bacterial cell?

(Multiple Choice)

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Question 7

Which of the following is absent in an operon?

(Multiple Choice)

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Question 8

Recent experimental evidence has shown that only one operator sequence is needed for maximal expression.

(True/False)

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Question 9

$\beta$ -galactosidase cleaves the disaccharide lactose into and __.

(Short Answer)

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Question 10

Identify the allosteric protein.

(Multiple Choice)

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Question 11

Which of the following statements is true about a lac operon with this genotype? I^-d O⁺ Z⁺ Y^- A^-

(Multiple Choice)

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Question 12

cAMP level is increased by the presence of high levels of glucose.

(True/False)

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Question 13

In the process of creating a trp operon mutant,the region for the riboswitch was accidentally deleted.Which of the following would be a likely observation in the transcription assays?

(Multiple Choice)

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(33)

Question 14

In the presence of glucose the lac operon is kept inactive by _ _.

(Short Answer)

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Question 15

__is the enzyme needed to bring lactose into the bacterial cell.

(Short Answer)

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Question 16

In an in vitro transcription assay to detect the level of transcription from a lac operon,one of the RNA precursors was tagged with a fluorescent label on the $\beta$ -phosphate.Which of the following is a likely outcome if transcription occurred?

(Multiple Choice)

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(35)

Question 17

Which of the following would be effective in blocking transcription from the lac operon in the presence of permease?

(Multiple Choice)

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Question 18

Which of the following bond creates the linkage between glucose and galactose?

(Multiple Choice)

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Question 19

Which of the following organisms would produce functional lac products in the presence of IPTG?

(Multiple Choice)

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Question 20

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Exam 7: Operons: Fine Control of Bacterial Transcription

One hypothesis explaining the mechanism of repression of the lac operon states that the repressor binds to the operator and blocks access by the polymerase to the adjacent promoter.

The ara operon is controlled by the _____________ protein.

Which of the following is true about the action of CAP at the lac promoter?

Which of the following is true about the regulation of the trp operon?

If an operon is under negative control it means that the operon is never operational.

Arabinose can derepress the ara operon by causing AraC to loosen its attachment to araO2 and to bind to araI2 instead.

Which of the following is most likely to occur if the level of glucose is low in a bacterial cell?

Which of the following is absent in an operon?

Recent experimental evidence has shown that only one operator sequence is needed for maximal expression.

β\betaβ -galactosidase cleaves the disaccharide lactose into __________ and ____________.

Identify the allosteric protein.

Which of the following statements is true about a lac operon with this genotype? I-d O+ Z+ Y- A-

cAMP level is increased by the presence of high levels of glucose.

In the process of creating a trp operon mutant,the region for the riboswitch was accidentally deleted.Which of the following would be a likely observation in the transcription assays?

In the presence of glucose the lac operon is kept inactive by _________ _________.

________ __________is the enzyme needed to bring lactose into the bacterial cell.

In an in vitro transcription assay to detect the level of transcription from a lac operon,one of the RNA precursors was tagged with a fluorescent label on the β\betaβ -phosphate.Which of the following is a likely outcome if transcription occurred?

Which of the following would be effective in blocking transcription from the lac operon in the presence of permease?

Which of the following bond creates the linkage between glucose and galactose?

Which of the following organisms would produce functional lac products in the presence of IPTG?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

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Arabinose can derepress the ara operon by causing AraC to loosen its attachment to araO₂ and to bind to araI₂ instead.

$\beta$ -galactosidase cleaves the disaccharide lactose into and __.

Which of the following statements is true about a lac operon with this genotype? I^-d O⁺ Z⁺ Y^- A^-

In the presence of glucose the lac operon is kept inactive by _ _.

__is the enzyme needed to bring lactose into the bacterial cell.

In an in vitro transcription assay to detect the level of transcription from a lac operon,one of the RNA precursors was tagged with a fluorescent label on the $\beta$ -phosphate.Which of the following is a likely outcome if transcription occurred?