Question 1

Picornavirus mRNAs are not capped,yet they can still out compete host mRNAs for binding to the ribosome by

Accepted Answer

A) inactivating host Cap binding protein,eIF4F. 
B) degrading host mRNA. 
C) having a stronger affinity for the ribosome. 
D) inactivation of host RNases. 
E) inactivation of host Cap binding protein,eIF2B. 
A) inactivating host Cap binding protein,eIF4F. 
B) degrading host mRNA. 
C) having a stronger affinity for the ribosome. 
D) inactivation of host RNases. 
E) inactivation of host Cap binding protein,eIF2B. A

Question 2

Match the following eukaryotic translation initiation factors with their correct function.
-This initiation factor binds to the 40S subunit and inhibits reassociation of the 40S and 60S subunits.C

Accepted Answer

A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02 
A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02 C

Question 3

Which of the following is not part of the 30S initiation complex?

Accepted Answer

A) IF1,IF2,and IF3 
B) 5S rRNA 
C) 16S rRNA 
D) amino-acyl tRNA 
E) mRNA 
A) IF1,IF2,and IF3 
B) 5S rRNA 
C) 16S rRNA 
D) amino-acyl tRNA 
E) mRNA B

Question 4

E.coli $\sigma$32 temperature sensitive expression is an example of transcriptional control.

Accepted Answer

A) True 
 B)False

Question 5

The let-7 miRNA blocks _________________ initiation in human cells.

Accepted Answer

The answer of The let-7 miRNA blocks _________________ initiation in...

Question 6

Match the following eukaryotic translation initiation factors with their correct function.
-This initiation factor binds to the 60S subunit and inhibits reassociation of the 40S and 60S subunits.E

Accepted Answer

A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02 
A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02

Question 7

Which of the following molecules does not interact with eIF4G?

Accepted Answer

A) Cap 
B) alternative protein(x) 
C) Pab1p 
D) eIF3 
E) eIF2 
A) Cap 
B) alternative protein(x) 
C) Pab1p 
D) eIF3 
E) eIF2

Question 8

There are _________ aminoacyl-tRNA synthetases,one for each amino acid.

Accepted Answer

The answer of There are _________ aminoacyl-tRNA synthetases,one for each...

Question 9

Most eukaryotic mRNAs have 5' caps that function in translation initiation instead of Shine -Dalgarno sequences found in prokaryotes.

Accepted Answer

A) True 
 B)False

Question 10

Match the following eukaryotic translation initiation factors with their correct function.
-This initiation factor aids in ribosome scanning to locate the initiation codon.B

Accepted Answer

A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02 
A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02

Question 11

Which of the following is the best definition of translation?

Accepted Answer

A) a process by which pre-mRNA is transformed into mature mRNA 
B) the process by which pre-mRNA is translated by ribozymes to produce proteins 
C) a process by which a gene is converted into mRNA 
D) the process by which ribosomes read the genetic message to produce a protein 
E) a process by which a gene is converted into a protein 
A) a process by which pre-mRNA is transformed into mature mRNA 
B) the process by which pre-mRNA is translated by ribozymes to produce proteins 
C) a process by which a gene is converted into mRNA 
D) the process by which ribosomes read the genetic message to produce a protein 
E) a process by which a gene is converted into a protein

Question 12

A(n)_______________ is defined as the area between an initiation codon and a downstream termination codon located in the same reading frame.

Accepted Answer

The answer of A(n)_______________ is defined as the area between...

Question 13

The _______ subunit of the E.coli ribosome binds the mRNA and tRNA while the _________ subunit has the peptidyl transferase activity.

Accepted Answer

The answer of The _______ subunit of the E.coli ribosome...

Question 14

In prokaryotes the first amino acid incorporated into a polypeptide is a __________.

Accepted Answer

N-formyl-m

Question 15

Similar to prokaryotes,eukaryotic translation incorporates an N-formyl-methionine as the first amino acid.

Accepted Answer

A) True 
 B)False

Question 16

Match the following eukaryotic translation initiation factors with their correct function.
-This initiation factor is a Cap binding protein.D

Accepted Answer

A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02 
A) eIF2 
B) eIF1 
C) eIF3 
D) eIF4F 
E) eIF6
Bloom's Level: 1.Remember
Section: 17.02

Question 17

eIF4A is a member of the DEAD protein family that has RNA helicase activity.

Accepted Answer

A) True 
 B)False

Question 18

Translation initiation in eukaryotes involves scanning of the mRNA to locate a favorable AUG.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following eukaryotic transcription factors is homologous to the prokaryotic transcription factor IF2?

Accepted Answer

A) eIF1A 
B) eIF2B 
C) eIF3 
D) eIF4G 
E) eIF5B 
A) eIF1A 
B) eIF2B 
C) eIF3 
D) eIF4G 
E) eIF5B

Question 20

eIF1 and eIF1A appear to encourage formation of a stable 48S complex by dissociating improper 40S and mRNA complexes.

Accepted Answer

A) True 
 B)False

Exam 17: The Mechanism of Translation I: Initiation

Picornavirus mRNAs are not capped,yet they can still out compete host mRNAs for binding to the ribosome by

Match the following eukaryotic translation initiation factors with their correct function. -This initiation factor binds to the 40S subunit and inhibits reassociation of the 40S and 60S subunits.C

Which of the following is not part of the 30S initiation complex?

E.coli σ\sigmaσ 32 temperature sensitive expression is an example of transcriptional control.

The let-7 miRNA blocks _________________ initiation in human cells.

Match the following eukaryotic translation initiation factors with their correct function. -This initiation factor binds to the 60S subunit and inhibits reassociation of the 40S and 60S subunits.E

Which of the following molecules does not interact with eIF4G?

There are _________ aminoacyl-tRNA synthetases,one for each amino acid.

Most eukaryotic mRNAs have 5' caps that function in translation initiation instead of Shine -Dalgarno sequences found in prokaryotes.

Match the following eukaryotic translation initiation factors with their correct function. -This initiation factor aids in ribosome scanning to locate the initiation codon.B

Which of the following is the best definition of translation?

A(n)_______________ is defined as the area between an initiation codon and a downstream termination codon located in the same reading frame.

The _______ subunit of the E.coli ribosome binds the mRNA and tRNA while the _________ subunit has the peptidyl transferase activity.

In prokaryotes the first amino acid incorporated into a polypeptide is a __________.

Similar to prokaryotes,eukaryotic translation incorporates an N-formyl-methionine as the first amino acid.

Match the following eukaryotic translation initiation factors with their correct function. -This initiation factor is a Cap binding protein.D

eIF4A is a member of the DEAD protein family that has RNA helicase activity.

Translation initiation in eukaryotes involves scanning of the mRNA to locate a favorable AUG.

Which of the following eukaryotic transcription factors is homologous to the prokaryotic transcription factor IF2?

eIF1 and eIF1A appear to encourage formation of a stable 48S complex by dissociating improper 40S and mRNA complexes.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

E.coli $\sigma$ ³² temperature sensitive expression is an example of transcriptional control.

The _ subunit of the E.coli ribosome binds the mRNA and tRNA while the ___ subunit has the peptidyl transferase activity.