Question 1

Which of the following is NOT a function of the mRNA cap?

Accepted Answer

A) protects the mRNA from degradation 
B) enhances translatability of the mRNA 
C) enhances transport of the mRNA to the cytoplasm 
D) enhances splicing of the mRNA 
E) helps regulate expression of the mRNA 
A) protects the mRNA from degradation 
B) enhances translatability of the mRNA 
C) enhances transport of the mRNA to the cytoplasm 
D) enhances splicing of the mRNA 
E) helps regulate expression of the mRNA E

Question 2

The capping substance for eukaryotic mRNAs is ______________.

Accepted Answer

7-methylguanosine

Question 3

The cap plays a role in the removal of the ___________ intron while poly(A)tail plays a role in the removal of the ____________ intron.

Accepted Answer

first,last

Question 4

Once a mRNA loses its poly(A)tail,it is imported back into the nucleus.

Accepted Answer

A) True 
 B)False

Question 5

Experiments using yeast as a model have shown that cleavage of the poly(A)tail is necessary for transcription termination.

Accepted Answer

A) True 
 B)False

Question 6

The addition of poly(A)to mRNA seems to be important for its translatability.

Accepted Answer

A) True 
 B)False

Question 7

Which of the following regions on calf-thymus poly(A)polymerase has been shown experimentally not to be necessary for its activity in vitro?

Accepted Answer

A) RNA binding domain 
B) polymerase module 
C) nuclear localization signals 
D) serine/threonine rich regions 
E) All domains are necessary. 
A) RNA binding domain 
B) polymerase module 
C) nuclear localization signals 
D) serine/threonine rich regions 
E) All domains are necessary.

Question 8

Which of the following is not required for mammalian cell polyadenylation of pre-mRNA?

Accepted Answer

A) PAP 
B) CPSF 
C) PABII 
D) RNA polymerase I 
E) RNA polymerase II 
A) PAP 
B) CPSF 
C) PABII 
D) RNA polymerase I 
E) RNA polymerase II

Question 9

Pre-mRNA must be cleaved before it is polyadenylated.

Accepted Answer

A) True 
 B)False

Question 10

At least 5 different cDNAs have been isolated for the enzyme poly(A)polymerase.These cDNAs are a result of alternative ___________ and ______________ of the poly(A)polymerase gene.

Accepted Answer

splicing,p

Question 11

Most genes do not encode for a poly(A)tail.

Accepted Answer

A) True 
 B)False

Question 12

When does capping of the pre-mRNA occur?

Accepted Answer

A) It is the first nucleotide added by RNA polymerase. 
B) It occurs before the mRNA reaches a chain length of 30 nt. 
C) It occurs after the poly(
A) tail has been added. 
D) It occurs after splicing has occurred. 
E) It occurs after export of the mRNA into the cytoplasm. 
A) It is the first nucleotide added by RNA polymerase. 
B) It occurs before the mRNA reaches a chain length of 30 nt. 
C) It occurs after the poly(
A) tail has been added. 
D) It occurs after splicing has occurred. 
E) It occurs after export of the mRNA into the cytoplasm.

Question 13

___________ RNA is the only type of RNA to get polyadenylated.

Accepted Answer

The answer of ___________ RNA is the only type of...

Question 14

Poly(A)polymerase can be found in the _____________ of a cell.

Accepted Answer

A) nucleus 
B) cytoplasm 
C) lysosomes 
D) nucleus and cytoplasm 
E) nucleus and lysosomes 
A) nucleus 
B) cytoplasm 
C) lysosomes 
D) nucleus and cytoplasm 
E) nucleus and lysosomes

Question 15

The poly(A)tail of eukaryotic mRNA is approximately ___________ nucleotides in length.

Accepted Answer

A) 5 
B) 25 
C) 100 
D) 250 
E) 1000 
A) 5 
B) 25 
C) 100 
D) 250 
E) 1000

Question 16

The function of a nucleotide phosphohydrolase is to ______________ phosphates from its substrate.

Accepted Answer

The answer of The function of a nucleotide phosphohydrolase is...

Question 17

RNA polymerase II transcription of a gene usually stops at the polyadenylation site.

Accepted Answer

A) True 
 B)False

Question 18

The 5' cap of mRNA helps protect the mature mRNA from degradation by ________.

Accepted Answer

The answer of The 5' cap of mRNA helps protect...

Question 19

Pre-mRNA processing begins during transcription.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following is the mRNA polyadenylation signal motif found in mammalian cells?

Accepted Answer

A) AAGAAA 
B) AAUAAA 
C) AAUGGG 
D) UUAUUU 
E) GGAUUU 
A) AAGAAA 
B) AAUAAA 
C) AAUGGG 
D) UUAUUU 
E) GGAUUU

Exam 15: Rna Processing II: Capping and Polyadenylation

Which of the following is NOT a function of the mRNA cap?

The capping substance for eukaryotic mRNAs is ______________.

The cap plays a role in the removal of the ___________ intron while poly(A)tail plays a role in the removal of the ____________ intron.

Once a mRNA loses its poly(A)tail,it is imported back into the nucleus.

Experiments using yeast as a model have shown that cleavage of the poly(A)tail is necessary for transcription termination.

The addition of poly(A)to mRNA seems to be important for its translatability.

Which of the following regions on calf-thymus poly(A)polymerase has been shown experimentally not to be necessary for its activity in vitro?

Which of the following is not required for mammalian cell polyadenylation of pre-mRNA?

Pre-mRNA must be cleaved before it is polyadenylated.

At least 5 different cDNAs have been isolated for the enzyme poly(A)polymerase.These cDNAs are a result of alternative ___________ and ______________ of the poly(A)polymerase gene.

Most genes do not encode for a poly(A)tail.

When does capping of the pre-mRNA occur?

___________ RNA is the only type of RNA to get polyadenylated.

Poly(A)polymerase can be found in the _____________ of a cell.

The poly(A)tail of eukaryotic mRNA is approximately ___________ nucleotides in length.

The function of a nucleotide phosphohydrolase is to ______________ phosphates from its substrate.

RNA polymerase II transcription of a gene usually stops at the polyadenylation site.

The 5' cap of mRNA helps protect the mature mRNA from degradation by ________.

Pre-mRNA processing begins during transcription.

Which of the following is the mRNA polyadenylation signal motif found in mammalian cells?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

The cap plays a role in the removal of the _ intron while poly(A)tail plays a role in the removal of the __ intron.

At least 5 different cDNAs have been isolated for the enzyme poly(A)polymerase.These cDNAs are a result of alternative _ and ____ of the poly(A)polymerase gene.