Question 1

Which technique is most likely to make use of reverse transcriptase?

Accepted Answer

A)  Homologous recombination 
B)  cDNA microarray 
C)  RNAi 
D)  Mutagenesis 
E)  Cell transplantation 
A)  Homologous recombination 
B)  cDNA microarray 
C)  RNAi 
D)  Mutagenesis 
E)  Cell transplantation B

Question 2

In the construction of an expression vector,a signal sequence

Accepted Answer

A)  allows for the expression of a gene in a particular tissue only. 
B)  allows the expression of a gene to be turned on or off. 
C)  allows the gene product to be directed to an appropriate location. 
D)  makes a gene responsive to hormonal stimulation. 
E)  allows for the expression of a gene in all types of tissues. 
A)  allows for the expression of a gene in a particular tissue only. 
B)  allows the expression of a gene to be turned on or off. 
C)  allows the gene product to be directed to an appropriate location. 
D)  makes a gene responsive to hormonal stimulation. 
E)  allows for the expression of a gene in all types of tissues. C

Question 3

An example of bioremediation is

Accepted Answer

A)  pharming. 
B)  genetically engineering a plant to express the Bt toxin. 
C)  selecting for genes that improve growth. 
D)  using microbes to treat wastewater. 
E)  implanting a surrogate ewe with an egg carrying a transgene. 
A)  pharming. 
B)  genetically engineering a plant to express the Bt toxin. 
C)  selecting for genes that improve growth. 
D)  using microbes to treat wastewater. 
E)  implanting a surrogate ewe with an egg carrying a transgene. D

Question 4

A biologist would most likely use the technique of electroporation to

Accepted Answer

A)  separate proteins based on size and charge. 
B)  knock out a gene. 
C)  create antisense DNA. 
D)  measure expression levels of a gene. 
E)  insert DNA into host cells. 
A)  separate proteins based on size and charge. 
B)  knock out a gene. 
C)  create antisense DNA. 
D)  measure expression levels of a gene. 
E)  insert DNA into host cells.

Question 5

Use the following to answer questions :
Suppose one restriction enzyme (enzyme A)makes two cuts in a linear DNA fragment and another restriction enzyme (enzyme B)makes one cut in that same DNA.The cuts are not in the same place,and none of the cut fragments are exactly the same size.The restriction digest is set up on an electrophoresis gel.
-How many bands should the gel pick up if both enzymes A and B are used in the restriction digest?

Accepted Answer

A)  Two 
B)  Three 
C)  Four 
D)  Five 
E)  Six 
A)  Two 
B)  Three 
C)  Four 
D)  Five 
E)  Six

Question 6

A promoter would most likely be used for

Accepted Answer

A)  constructing a gene library. 
B)  constructing an expression vector. 
C)  performing a restriction digestion. 
D)  attempting to "silence" a gene. 
E)  constructing a recombinant plasmid. 
A)  constructing a gene library. 
B)  constructing an expression vector. 
C)  performing a restriction digestion. 
D)  attempting to "silence" a gene. 
E)  constructing a recombinant plasmid.

Question 7

Which is the best example of an inducible promoter?

Accepted Answer

A)  A promoter that responds only in liver tissue 
B)  A promoter that responds only when juvenile hormone is present 
C)  A promoter that responds all the time,regardless of conditions 
D)  A promoter that possesses signal sequences 
E)  A promoter that produces erythropoietin 
A)  A promoter that responds only in liver tissue 
B)  A promoter that responds only when juvenile hormone is present 
C)  A promoter that responds all the time,regardless of conditions 
D)  A promoter that possesses signal sequences 
E)  A promoter that produces erythropoietin

Question 8

To replicate within the cells of a host,recombinant DNA must either _______ into the host's genome or contain a(n)_______.Otherwise,the recombinant DNA would not be replicated,since _______ requires specific sequences to bind to DNA.

Accepted Answer

A)  integrate;origin of replication;DNA polymerase 
B)  integrate;vector;DNA polymerase 
C)  recombine;origin of replication;DNA ligase 
D)  recombine;stop transcription signal;DNA polymerase 
E)  integrate;stop transcription signal;DNA ligase 
A)  integrate;origin of replication;DNA polymerase 
B)  integrate;vector;DNA polymerase 
C)  recombine;origin of replication;DNA ligase 
D)  recombine;stop transcription signal;DNA polymerase 
E)  integrate;stop transcription signal;DNA ligase

Question 9

There are some limitations to the use of bacteria as hosts in studies of eukaryotic gene expression because

Accepted Answer

A)  bacteria are haploid. 
B)  transcription works differently in bacteria and in eukaryotes. 
C)  bacteria have a small genome size. 
D)  eukaryotes contain plasmids. 
E)  bacteria lack genetic markers. 
A)  bacteria are haploid. 
B)  transcription works differently in bacteria and in eukaryotes. 
C)  bacteria have a small genome size. 
D)  eukaryotes contain plasmids. 
E)  bacteria lack genetic markers.

Question 10

Suppose a DNA fragment from a human gene has been cut such that it has sticky ends.A DNA fragment from bacteria with sticky ends complementary to those of the human DNA is added to the mix.What would prevent the complementary ends of the human DNA from rejoining,and thus prevent the formation of a recombinant DNA molecule?

Accepted Answer

A)  DNA ligase 
B)  Restriction endonuclease EcoRI 
C)  Reverse transcriptase 
D)  A cloning vector 
E)  Nothing 
A)  DNA ligase 
B)  Restriction endonuclease EcoRI 
C)  Reverse transcriptase 
D)  A cloning vector 
E)  Nothing

Question 11

DNA,because it has a _______ charge,moves to the _______ end of the field in gel electrophoresis;_______ DNA molecules migrate the most quickly.

Accepted Answer

A)  positive;positive;smaller 
B)  positive;positive;larger 
C)  positive;negative;smaller 
D)  negative;positive;larger 
E)  negative;positive;smaller 
A)  positive;positive;smaller 
B)  positive;positive;larger 
C)  positive;negative;smaller 
D)  negative;positive;larger 
E)  negative;positive;smaller

Question 12

Which statement about genomic and cDNA libraries is true?

Accepted Answer

A)  A genomic library constructed from human brain tissue will likely be the same as a genomic library constructed from human pancreatic tissue. 
B)  A cDNA library constructed from human brain tissue will likely be the same as a cDNA library constructed from human pancreatic tissue. 
C)  A cDNA library would be very useful in examining noncoding DNA,whereas a genomic library would not be very useful. 
D)  Both cDNA and genomic libraries would be very useful in examining noncoding DNA. 
E)  A genomic library would be very useful in examining coding DNA,whereas a cDNA library would not be very useful. 
A)  A genomic library constructed from human brain tissue will likely be the same as a genomic library constructed from human pancreatic tissue. 
B)  A cDNA library constructed from human brain tissue will likely be the same as a cDNA library constructed from human pancreatic tissue. 
C)  A cDNA library would be very useful in examining noncoding DNA,whereas a genomic library would not be very useful. 
D)  Both cDNA and genomic libraries would be very useful in examining noncoding DNA. 
E)  A genomic library would be very useful in examining coding DNA,whereas a cDNA library would not be very useful.

Question 13

Two plasmids are used in a recombinant DNA procedure.Plasmid 67A carries a gene for resistance to the antibiotic ciprofloxacin,and plasmid 65B carries a gene for resistance to the antibiotic tetracycline.The plasmids are cut with HindIII restriction endonuclease,and then mixed with DNA ligase.In this procedure,what is the role of tetracycline?

Accepted Answer

A)  It enhances transformation. 
B)  It is a selectable marker. 
C)  It is a vector. 
D)  It ligates the DNAs. 
E)  It provides color to the bacterial colonies. 
A)  It enhances transformation. 
B)  It is a selectable marker. 
C)  It is a vector. 
D)  It ligates the DNAs. 
E)  It provides color to the bacterial colonies.

Question 14

The process of inserting recombinant DNA is considered transfection when

Accepted Answer

A)  the host cells are mouse tissue culture cells. 
B)  the host cells are E.coli cells. 
C)  the host cells are yeast cells. 
D)  the vector lacks a replicon. 
E)  a selectable marker is used. 
A)  the host cells are mouse tissue culture cells. 
B)  the host cells are E.coli cells. 
C)  the host cells are yeast cells. 
D)  the vector lacks a replicon. 
E)  a selectable marker is used.

Question 15

Which property would distinguish an expression vector from other vectors?

Accepted Answer

A)  Inclusion of reverse transcriptase 
B)  A recognition sequence for a restriction enzyme 
C)  The ability to replicate independently within the host 
D)  A reporter gene 
E)  Transcription binding sites 
A)  Inclusion of reverse transcriptase 
B)  A recognition sequence for a restriction enzyme 
C)  The ability to replicate independently within the host 
D)  A reporter gene 
E)  Transcription binding sites

Question 16

Use the following to answer questions :
A plasmid has genes that confer resistance to both ampicillin and tetracycline.Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.
-A cell transformed with the resulting recombinant plasmid should be _______ to ampicillin and _______ to tetracycline.

Accepted Answer

A)  resistant;resistant 
B)  resistant;sensitive 
C)  resistant;complementary 
D)  sensitive;resistant 
E)  sensitive;complementary 
A)  resistant;resistant 
B)  resistant;sensitive 
C)  resistant;complementary 
D)  sensitive;resistant 
E)  sensitive;complementary

Question 17

A strain of corn is genetically engineered to tolerate the low pH of fields that are regularly exposed to severe acid rain.This is an example of

Accepted Answer

A)  the production of a transgenic crop that is adapted to its environment. 
B)  tailoring the environment to the needs of crop plants. 
C)  pharming. 
D)  the principle of genetic determinism. 
E)  the use of homologous recombination in biotechnology. 
A)  the production of a transgenic crop that is adapted to its environment. 
B)  tailoring the environment to the needs of crop plants. 
C)  pharming. 
D)  the principle of genetic determinism. 
E)  the use of homologous recombination in biotechnology.

Question 18

Restriction enzymes recognize and cut at DNA sequences that are _______ (i.e. ,that read the same way in both directions).

Accepted Answer

The answer of Restriction enzymes recognize and cut at DNA...

Question 19

Which outcome could not be accomplished through pharming?

Accepted Answer

A)  Mass production of a hormone that is a polypeptide 
B)  Mass production of a molecule that is a carbohydrate 
C)  Mass production of insulin 
D)  Mass production of a neurotransmitter that is a protein 
E)  Mass production of a prokaryotic protein 
A)  Mass production of a hormone that is a polypeptide 
B)  Mass production of a molecule that is a carbohydrate 
C)  Mass production of insulin 
D)  Mass production of a neurotransmitter that is a protein 
E)  Mass production of a prokaryotic protein

Question 20

Which plasmid would allow a researcher to visually select a bacterial colony that has a recombinant plasmid using white light? [Note: The plasmid also has selectable reporter gene for ampicillin resistance (ampr)outside of the cloning site.]

Accepted Answer

A)  A plasmid with the gene for X-gal in the cloning site 
B)  A plasmid with the gene for green fluorescent protein (GFP)outside of the cloning site 
C)  A plasmid with the LacZ reporter gene outside of the cloning site 
D)  A plasmid with the LacZ reporter gene in the cloning site 
E)  A plasmid with the green fluorescent protein (GFP)within the cloning site 
A)  A plasmid with the gene for X-gal in the cloning site 
B)  A plasmid with the gene for green fluorescent protein (GFP)outside of the cloning site 
C)  A plasmid with the LacZ reporter gene outside of the cloning site 
D)  A plasmid with the LacZ reporter gene in the cloning site 
E)  A plasmid with the green fluorescent protein (GFP)within the cloning site

Exam 13: Biotechnology

Which technique is most likely to make use of reverse transcriptase?

In the construction of an expression vector,a signal sequence

An example of bioremediation is

A biologist would most likely use the technique of electroporation to

A promoter would most likely be used for

Which is the best example of an inducible promoter?

To replicate within the cells of a host,recombinant DNA must either _______ into the host's genome or contain a(n)_______.Otherwise,the recombinant DNA would not be replicated,since _______ requires specific sequences to bind to DNA.

There are some limitations to the use of bacteria as hosts in studies of eukaryotic gene expression because

DNA,because it has a _______ charge,moves to the _______ end of the field in gel electrophoresis;_______ DNA molecules migrate the most quickly.

Which statement about genomic and cDNA libraries is true?

The process of inserting recombinant DNA is considered transfection when

Which property would distinguish an expression vector from other vectors?

A strain of corn is genetically engineered to tolerate the low pH of fields that are regularly exposed to severe acid rain.This is an example of

Restriction enzymes recognize and cut at DNA sequences that are _______ (i.e. ,that read the same way in both directions).

Which outcome could not be accomplished through pharming?

Which plasmid would allow a researcher to visually select a bacterial colony that has a recombinant plasmid using white light? [Note: The plasmid also has selectable reporter gene for ampicillin resistance (ampr)outside of the cloning site.]

Introduction

The Chemistry and Energy of Life

Nucleic Acids, Proteins, and Enzymes

Cells: the Working Units of Life

Cell Membranes and Signaling

Pathways That Harvest and Store Chemical Energy

The Cell Cycle and Cell Division

Inheritance, Genes, and Chromosomes

DNA and Its Role in Heredity

From Dna to Protein: Gene Expression

Regulation of Gene Expression

Genomes

Genes, Development, and Evolution

Processes of Evolution

Reconstructing and Using Phylogenies

Speciation

The History of Life on Earth

Bacteria, Archaea, and Viruses

The Origin and Diversification of Eukaryotes

The Evolution of Plants

The Evolution and Diversity of Fungi

Animal Origins and Diversity

The Plant Body

Plant Nutrition and Transport

Plant Growth and Development

Reproduction of Flowering Plants

Plants in the Environment

Fundamentals of Animal Function

Nutrition and Digestion

Breathing

Circulation

Muscle and Movement

Neurons, Sense Organs, and Nervous Systems

Nervous and Endocrine Control

Water and Salts: Excretion

Animal Reproduction

Animal Development

Immunology: Animal Defense Systems

Animal Behavior

The Distribution of Earths Ecological Systems

Populations

Ecological and Evolutionary Consequences of Interactions Within and Among Species

Ecological Communities

The Global Ecosystem

Filters

To replicate within the cells of a host,recombinant DNA must either ___ into the host's genome or contain a(n)_.Otherwise,the recombinant DNA would not be replicated,since _____ requires specific sequences to bind to DNA.

DNA,because it has a ___ charge,moves to the _ end of the field in gel electrophoresis;_____ DNA molecules migrate the most quickly.

Which plasmid would allow a researcher to visually select a bacterial colony that has a recombinant plasmid using white light? [Note: The plasmid also has selectable reporter gene for ampicillin resistance (amp^r)outside of the cloning site.]