Exam 14: DNA Structure, Replication, and Organization

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DNA ligase closes nicks between ____ by forming ____ bonds. ​

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In the Hershey and Chase experiment, 32P was used to label ____ and 35S was used to label ____.

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Identification
For the following questions, match the enzyme involved in DNA replication with its function.
Premises:
Main enzyme that extends RNA primer by adding DNA nucleotides to it
Responses:
DNA polymerase I
topoisomerase
single-stranded binding proteins
Correct Answer:
Verified
Premises:
Responses:
Main enzyme that extends RNA primer by adding DNA nucleotides to it
DNA polymerase I
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The genome of an organism contains 14% guanine; therefore, it's genome also contains ____% thymine and ____% cytosine. ​

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Labeling
Examine the following figure. Match each letter of the figure with the appropriate DNA replication protein or enzyme. Labeling  Examine the following figure. Match each letter of the figure with the appropriate DNA replication protein or enzyme.       Figure 14.2

Figure 14.2
Premises:
single-stranded binding protein
Responses:
A
C
D
Correct Answer:
Verified
Premises:
Responses:
single-stranded binding protein
A
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Some research has indicated that increased exercise is linked to increased telomere length in renewable tissues, like blood. Assuming that telomerase levels are not increased by exercise, what might explain the increase in telomere length?

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The two strands of a DNA double helix are said to be antiparallel. This means that ____. ​

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Cancer cells ____. ​

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Describe what happens to the nucleosome during DNA replication.

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Labeling
Examine the following figure. Match each letter of the figure with the appropriate DNA replication protein or enzyme. Labeling  Examine the following figure. Match each letter of the figure with the appropriate DNA replication protein or enzyme.       Figure 14.2

Figure 14.2
Premises:
primase
Responses:
C
D
B
Correct Answer:
Verified
Premises:
Responses:
primase
C
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Helicase ____. ​

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Avery, MacLeod, and McCarty showed that DNA was the transforming principle by demonstrating that mice survived when injected with a mixture of dead virulent bacteria and live nonvirulent bacteria that were treated with a DNA degrading enzyme. If RNA was the genetic material, what would have been the effect on the mice? Why?

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Proofreading by ____ corrects errors that occur during DNA replication. ​

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Identification
For the following questions, match the enzyme involved in DNA replication with its function.
Premises:
Synthesizes short RNA segment to initiate new DNA strand
Responses:
sliding clamp
DNA ligase
helicase
Correct Answer:
Verified
Premises:
Responses:
Synthesizes short RNA segment to initiate new DNA strand
sliding clamp
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The transforming principle described by Griffith in his work with Streptococcus pneumoniae is ____. ​

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Which statement correctly describes DNA base pairing?

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What are two main reasons why DNA mutations are so rare?

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Reiji Okazaki discovered what are now called "Okazaki fragments" produced during DNA replication. These fragments are ____. ​

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Individuals with xeroderma pigmentosum inherit a faulty DNA repair mechanism. As a consequence, ____.

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Why is DNA ligase most active on the lagging strand during DNA replication? ​

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