Exam 18: Dna Technologies: Making and Using Genetically Altered Organisms, and Other Applications

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The technique by which DNA, RNA, or protein molecules are separated in a gel that is subjected to an electric field is called ____. ​

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E

Match each of the following terms with its correct definition.
Premises:
biotechnology
Responses:
Any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose.
An international agreement on the safe use and handling of GMOs.
DNA from two different sources that have been joined together into a single molecule.
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Verified
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Responses:
biotechnology
Any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose.
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Restriction fragment length polymorphisms (RFLPs)are used to ____. ​

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B

Different methodologies can be used to obtain the same information about a DNA sequence. ​

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Match each of the following terms with its correct definition.
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short tandem repeat
Responses:
This term is used to describe organisms that have been modified to contain genetic information from an external source.
Using STR loci, this technique revealed that the source of Dolly's genomic DNA was the ewe who supplied the mammary cell
Bacterial enzymes that recognize and cut specific DNA sequences.
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short tandem repeat
This term is used to describe organisms that have been modified to contain genetic information from an external source.
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    Figure 18.1 Answer the question using the accompanying figure. What does letter E represent? ​   Figure 18.1 Answer the question using the accompanying figure. What does letter E represent? ​

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Reverse transcriptase creates a single-stranded DNA molecule that is complementary to an mRNA template. ​

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A researcher is interested in studying gene X, but not gene Y, in Drosophila . What does she need to amplify only gene X from the Drosophila genome? ​

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How do scientists determine the annealing temperature used in PCR reactions? ​

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Match each of the following terms with its correct definition.
Premises:
Cartagena Protocol on Biosafety
Responses:
An international agreement on the safe use and handling of GMOs.
Short 2-6 bp sequences repeated in series.
DNA from two different sources that have been joined together into a single molecule.
Correct Answer:
Verified
Premises:
Responses:
Cartagena Protocol on Biosafety
An international agreement on the safe use and handling of GMOs.
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Metabolic fingerprinting could be used ____. ​

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Germline and somatic gene therapy are used to treat human genetic diseases. ​

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The key elements of a PCR reaction are ____. ​

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DNA fingerprinting is a method used to ____. ​

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Transgenic organisms contain extra copies of their own genes.

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In agarose gel electrophoresis, DNA molecules move towards the ____ pole because they ____. ​

(Multiple Choice)
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Match each of the following terms with its correct definition.
Premises:
DNA fingerprinting
Responses:
Short 2-6 bp sequences repeated in series.
Using STR loci, this technique revealed that the source of Dolly's genomic DNA was the ewe who supplied the mammary cell
DNA technology used to alter genes in a cell or organism.
Correct Answer:
Verified
Premises:
Responses:
DNA fingerprinting
Short 2-6 bp sequences repeated in series.
(Matching)
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Match each of the following terms with its correct definition.
Premises:
restriction endonucleases
Responses:
DNA technology used to alter genes in a cell or organism.
This technique is used to separate DNA molecules based on their relative sizes.
DNA from two different sources that have been joined together into a single molecule.
Correct Answer:
Verified
Premises:
Responses:
restriction endonucleases
DNA technology used to alter genes in a cell or organism.
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    Figure 18.1 Answer the question using the accompanying figure. Of the fragments labeled A-D, which is closest to the positive electrode? ​   Figure 18.1 Answer the question using the accompanying figure. Of the fragments labeled A-D, which is closest to the positive electrode? ​

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The phases of each PCR cycle listed in order are ____. ​

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