Question 1

Crops that are resistant to the herbicide Roundup ____. ​

Accepted Answer

A) express the enzyme degraded by Roundup at high levels so that not all of the enzyme is inhibited 
B) have an extra coating outside their cell wall that inhibits Roundup from entering the cells 
C) contain a bacterial version of the chloroplast enzyme inhibited by Roundup 
D) produce a compound that degrades Roundup 
E) do not have the membrane channels through which Roundup enters the plant 
A) express the enzyme degraded by Roundup at high levels so that not all of the enzyme is inhibited 
B) have an extra coating outside their cell wall that inhibits Roundup from entering the cells 
C) contain a bacterial version of the chloroplast enzyme inhibited by Roundup 
D) produce a compound that degrades Roundup 
E) do not have the membrane channels through which Roundup enters the plant

Question 2

The DNA sequence recognized by restriction endonucleases is generally ____ base pairs long. ​

Accepted Answer

A) 1-2 
B) 4-8 
C) 10-15 
D) 24-32 
E) 36-47 
A) 1-2 
B) 4-8 
C) 10-15 
D) 24-32 
E) 36-47

Question 3

Define the purpose of inserting a gene into a plasmid cloning vector.

Accepted Answer

After inserting a gene into a cloning ve

Question 4

The characterization of whole genomes, including their structures (sequences), functions, and evolution is called ____. ​

Accepted Answer

A) genetic engineering 
B) genomics 
C) bioinformatics 
D) biotechnology 
A) genetic engineering 
B) genomics 
C) bioinformatics 
D) biotechnology

Question 5

Gene therapy is used to treat many diseases.

Accepted Answer

A) True 
 B)False

Question 6

How is DNA introduced into plants using the Ti plasmid? ​

Accepted Answer

A) The gene of interest remains in the Ti plasmid after the plasmid has transformed the plant, and the gene is expressed in the plant. 
B) The Ti plasmid inserts the gene of interest directly into the plant's nuclear DNA. 
C) The entire Ti plasmid including the gene of interest is inserted into the plant's nuclear DNA. 
D) The Ti plasmid inserts the foreign DNA into the plant's chloroplast DNA. 
E) The Ti plasmid inserts the foreign DNA into the plant's mitochondrial DNA. 
A) The gene of interest remains in the Ti plasmid after the plasmid has transformed the plant, and the gene is expressed in the plant. 
B) The Ti plasmid inserts the gene of interest directly into the plant's nuclear DNA. 
C) The entire Ti plasmid including the gene of interest is inserted into the plant's nuclear DNA. 
D) The Ti plasmid inserts the foreign DNA into the plant's chloroplast DNA. 
E) The Ti plasmid inserts the foreign DNA into the plant's mitochondrial DNA.

Question 7

Mass quantities of a target DNA sequence can be obtained by ____. ​

Accepted Answer

A) restriction endonuclease digestion only 
B) gene cloning only 
C) PCR only 
D) restriction endonuclease digestion and PCR 
E) gene cloning and PCR 
A) restriction endonuclease digestion only 
B) gene cloning only 
C) PCR only 
D) restriction endonuclease digestion and PCR 
E) gene cloning and PCR

Question 8

The Ti plasmid is used to ____. ​

Accepted Answer

A) create clones of mammals 
B) treat human diseases by somatic gene therapy 
C) insert transgenes into bacteria 
D) insert transgenes into plants 
E) insert transgenes into animals 
A) create clones of mammals 
B) treat human diseases by somatic gene therapy 
C) insert transgenes into bacteria 
D) insert transgenes into plants 
E) insert transgenes into animals

Question 9

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. Blue colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ+ gene is ____. ​

Accepted Answer

A) recombinant; deleted from the plasmid 
B) nonrecombinant; intact 
C) recombinant; intact 
D) nonrecombinant; disrupted by the inserted DNA fragment 
E) recombinant; disrupted by the inserted DNA fragment 
A) recombinant; deleted from the plasmid 
B) nonrecombinant; intact 
C) recombinant; intact 
D) nonrecombinant; disrupted by the inserted DNA fragment 
E) recombinant; disrupted by the inserted DNA fragment

Question 10

What distinguishes expression vectors from other cloning vectors? ​

Accepted Answer

A) Expression vectors do not have an origin of replication. 
B) Only expression vectors have origins of replication. 
C) Expression vectors contain regulatory sequences to allow transcription and translation of an inserted gene. 
D) Inserted genes cannot be transcribed from an expression vector. 
E) Expression vectors do not contain antibiotic resistance genes. 
A) Expression vectors do not have an origin of replication. 
B) Only expression vectors have origins of replication. 
C) Expression vectors contain regulatory sequences to allow transcription and translation of an inserted gene. 
D) Inserted genes cannot be transcribed from an expression vector. 
E) Expression vectors do not contain antibiotic resistance genes.

Question 11

The CRISPR-Cas system is exploited experimentally for ____. ​

Accepted Answer

A) programmable RNA-guided genome editing 
B) programmable DNA-guided genome editing 
C) cutting DNA at specific sites so it can be inserted into a cloning vector 
D) increasing transcription of a gene of interest 
E) enhancing DNA polymerase activity in PCR reactions 
A) programmable RNA-guided genome editing 
B) programmable DNA-guided genome editing 
C) cutting DNA at specific sites so it can be inserted into a cloning vector 
D) increasing transcription of a gene of interest 
E) enhancing DNA polymerase activity in PCR reactions

Question 12

Gel electrophoresis can only be used to analyze DNA samples. ​

Accepted Answer

A) True 
 B)False

Question 13

I need to determine if the gene for a particular protein is the same length in frogs, humans, and trees. Which method(s)will be most helpful to me? ​

Accepted Answer

A) PCR and agarose gel electrophoresis 
B) DNA cloning and PCR 
C) DNA cloning only 
D) PCR only 
E) agarose gel electrophoresis only 
A) PCR and agarose gel electrophoresis 
B) DNA cloning and PCR 
C) DNA cloning only 
D) PCR only 
E) agarose gel electrophoresis only

Question 14

When cloning DNA into bacteria, the ____. ​

Accepted Answer

A) DNA sequence is inserted into a plasmid 
B) DNA sequence is inserted into the bacterial chromosome 
C) linear DNA sequence is circularized, thus generating a plasmid 
D) new DNA is directly introduced into the bacterial cell 
E) approach depends on the species of host bacteria used in the experiment 
A) DNA sequence is inserted into a plasmid 
B) DNA sequence is inserted into the bacterial chromosome 
C) linear DNA sequence is circularized, thus generating a plasmid 
D) new DNA is directly introduced into the bacterial cell 
E) approach depends on the species of host bacteria used in the experiment

Question 15

Discuss the reasons why cloning transgenic animals may be preferable over traditional breeding. What are some of the problems with animal cloning?

Accepted Answer

Making transgenic mammals is expensive a

Question 16

Bioinformatics combines the fields of ____ to extract information from biological data. ​

Accepted Answer

A) computer science and social science 
B) mathematics and social science 
C) mathematics and computer science 
D) biology and history 
E) biology and physics 
A) computer science and social science 
B) mathematics and social science 
C) mathematics and computer science 
D) biology and history 
E) biology and physics

Question 17

Match between columns
                        Premises: transgenic

Accepted Answer

The Answer of Bacterial enzymes that recognize and cut specific is...

Question 18

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. White colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ+ gene is ____. ​

Accepted Answer

A) recombinant; deleted from the plasmid 
B) nonrecombinant; intact 
C) recombinant; intact 
D) nonrecombinant; disrupted by the inserted DNA fragment 
E) recombinant; disrupted by the inserted DNA fragment 
A) recombinant; deleted from the plasmid 
B) nonrecombinant; intact 
C) recombinant; intact 
D) nonrecombinant; disrupted by the inserted DNA fragment 
E) recombinant; disrupted by the inserted DNA fragment

Question 19

Which feature of a plasmid cloning vector is necessary for the plasmid to be replicated inside a host cell? ​

Accepted Answer

A) lacZ+ gene 
B) ampicillin resistance gene 
C) origin of replication 
D) ribosomal binding site 
E) restriction site 
A) lacZ+ gene 
B) ampicillin resistance gene 
C) origin of replication 
D) ribosomal binding site 
E) restriction site

Question 20

In which situation would gene cloning occur? ​

Accepted Answer

A) a DNA fragment is inserted directly into a bacterium and is replicated 
B) a DNA fragment is inserted into a plasmid, which is then introduced into a bacterium where the gene is expressed but the plasmid does not replicate 
C) a DNA fragment is inserted into a plasmid, which is then introduced to a bacterium where the plasmid replicates 
D) a DNA fragment is inserted directly into a bacterium and is digested by nucleases 
E) a protein of interest is taken up by a bacterium, which incorporates the protein into its cell membrane 
A) a DNA fragment is inserted directly into a bacterium and is replicated 
B) a DNA fragment is inserted into a plasmid, which is then introduced into a bacterium where the gene is expressed but the plasmid does not replicate 
C) a DNA fragment is inserted into a plasmid, which is then introduced to a bacterium where the plasmid replicates 
D) a DNA fragment is inserted directly into a bacterium and is digested by nucleases 
E) a protein of interest is taken up by a bacterium, which incorporates the protein into its cell membrane

Exam 18: Dna Technologies: Making and Using Genetically Altered Organisms, and Other Applications

Crops that are resistant to the herbicide Roundup ____. ​

The DNA sequence recognized by restriction endonucleases is generally ____ base pairs long. ​

Define the purpose of inserting a gene into a plasmid cloning vector.

The characterization of whole genomes, including their structures (sequences), functions, and evolution is called ____. ​

Gene therapy is used to treat many diseases.

How is DNA introduced into plants using the Ti plasmid? ​

Mass quantities of a target DNA sequence can be obtained by ____. ​

The Ti plasmid is used to ____. ​

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. Blue colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ+ gene is ____. ​

What distinguishes expression vectors from other cloning vectors? ​

The CRISPR-Cas system is exploited experimentally for ____. ​

Gel electrophoresis can only be used to analyze DNA samples. ​

I need to determine if the gene for a particular protein is the same length in frogs, humans, and trees. Which method(s)will be most helpful to me? ​

When cloning DNA into bacteria, the ____. ​

Discuss the reasons why cloning transgenic animals may be preferable over traditional breeding. What are some of the problems with animal cloning?

Bioinformatics combines the fields of ____ to extract information from biological data. ​

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. White colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ+ gene is ____. ​

Which feature of a plasmid cloning vector is necessary for the plasmid to be replicated inside a host cell? ​

In which situation would gene cloning occur? ​

Introduction to Biological Concepts and Research

Life, Chemistry, and Water

Biological Molecules: the Carbon Compounds of Life

Cells

Membranes and Transport

Energy, Enzymes, and Biological Reactions

Cellular Respiration: Harvesting Chemical Energy

Photosynthesis

Cell Communication

Cell Division and Mitosis

Meiosis: the Cellular Basis of Sexual Reproduction

Mendel, Genes, and Inheritance

Genes, Chromosomes, and Human Genetics

DNA Structure, Replication, and Organization

From DNA to Protein

Regulation of Gene Expression

Bacterial and Viral Genetics

Genomes and Proteomes

The Development of Evolutionary Thought

Microevolution: Genetic Changes Within Populations

Speciation

Paleobiology and Macroevolution

Systematic Biology: Phylogeny and Classification

The Origin of Life

Prokaryotes and Viruses

Protists

Seedless Plants

Seed Plants

Fungi

Animal Phylogeny, Acoelomates, and Protostomes

Deuterostomes: Vertebrates and Their Closest Relatives

The Plant Body

Transport in Plants

Plant Nutrition

Reproduction and Development in Flowering Plants

Plant Signals and Responses to the Environment

Introduction to Animal Organization and Physiology

Information Flow and the Neuron

Nervous Systems

Sensory Systems

The Endocrine System

Muscles, Bones, and Body Movements

The Circulatory System

Defenses Against Disease

Gas Exchange: the Respiratory System

Animal Nutrition

Regulating the Internal Environment

Animal Reproduction

Animal Development

Ecology and the Biosphere

Population Ecology

Population Interactions and Community Ecology

Ecosystems

Biodiversity and Conservation Biology

Animal Behavior

Filters

Crops that are resistant to the herbicide Roundup ____.

The DNA sequence recognized by restriction endonucleases is generally ____ base pairs long.

The characterization of whole genomes, including their structures (sequences), functions, and evolution is called ____.

How is DNA introduced into plants using the Ti plasmid?

Mass quantities of a target DNA sequence can be obtained by ____.

The Ti plasmid is used to ____.

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. Blue colonies on the plate contain bacteria with a plasmid, meaning the lacZ+ gene is .

What distinguishes expression vectors from other cloning vectors?

The CRISPR-Cas system is exploited experimentally for ____.

Gel electrophoresis can only be used to analyze DNA samples.

I need to determine if the gene for a particular protein is the same length in frogs, humans, and trees. Which method(s)will be most helpful to me?

When cloning DNA into bacteria, the ____.

Bioinformatics combines the fields of ____ to extract information from biological data.

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. White colonies on the plate contain bacteria with a plasmid, meaning the lacZ+ gene is .

Which feature of a plasmid cloning vector is necessary for the plasmid to be replicated inside a host cell?

In which situation would gene cloning occur?